scholarly journals Detection of Stimulated Erythropoiesis by the RNA-Based 5'-Aminolevulinate Synthase 2 Biomarker in Dried Blood Spot Samples

2019 ◽  
Vol 65 (12) ◽  
pp. 1563-1571 ◽  
Author(s):  
Olivier Salamin ◽  
Emeric Gottardo ◽  
Céline Schobinger ◽  
Gemma Reverter-Branchat ◽  
Jordi Segura ◽  
...  
Keyword(s):  
Recombinant Human Erythropoietin ◽  
Hematological Parameters ◽  
Venous Blood ◽  
Dried Blood Spots ◽  
Storage Conditions ◽  
Blood Collection ◽  
Aminolevulinate Synthase ◽  
Human Erythropoietin ◽  
Biological Passport ◽  
New Biomarkers

Abstract BACKGROUND Despite implementation of the Athlete Biological Passport 10 years ago, blood doping remains difficult to detect. Thus, there is a need for new biomarkers to increase the sensitivity of the adaptive model. Transcriptomic biomarkers originating from immature reticulocytes may be reliable indicators of blood manipulations. Furthermore, the use of dried blood spots (DBSs) for antidoping purposes constitutes a complementary approach to venous blood collection. Here, we developed a method of quantifying the RNA-based 5′-aminolevulinate synthase 2 (ALAS2) biomarker in DBS. MATERIALS The technical, interindividual, and intraindividual variabilities of the method, and the effects of storage conditions on the production levels of ALAS2 RNA were assessed. The method was used to monitor erythropoiesis stimulated endogenously (blood withdrawal) or exogenously (injection of recombinant human erythropoietin). RESULTS When measured over a 7-week period, the intra- and interindividual variabilities of ALAS2 expression in DBS were 12.5%–42.4% and 49%, respectively. Following withdrawal of 1 unit of blood, the ALAS2 RNA in DBS increased significantly for up to 15 days. Variations in the expression level of this biomarker in DBS samples were more marked than those of the conventional hematological parameters, reticulocyte percentage and immature reticulocyte fraction. After exogenous stimulation of erythropoiesis via recombinant human erythropoietin injection, ALAS2 expression in DBS increased by a mean 8-fold. CONCLUSIONS Monitoring of transcriptomic biomarkers in DBS could complement the measurement of hematological parameters in the Athlete Biological Passport and aid the detection of blood manipulations.

Effects of altitude and recombinant human erythropoietin on iron metabolism: a randomized controlled trial

2021 ◽  
Author(s):  
Andreas Breenfeldt Andersen ◽  
Thomas Christian Bonne ◽  
Jacob Bejder ◽  
Grace Jung ◽  
Tomas Ganz ◽  
...  
Keyword(s):  
Sea Level ◽  
Clinical Relevance ◽  
Recombinant Human Erythropoietin ◽  
Controlled Trial ◽  
Venous Blood ◽  
Early Assessment ◽  
Stress Erythropoiesis ◽  
Human Erythropoietin ◽  
The Impact

Current markers of iron deficiency (ID) such as ferritin and hemoglobin have shortcomings, and hepcidin and erythroferrone (ERFE) could be of clinical relevance in relation to early assessment of ID. Here, we evaluate whether exposure to altitude-induced hypoxia (2,320 m) alone, or in combination with recombinant human erythropoietin (rHuEPO) treatment, affects hepcidin and ERFE levels before alterations in routine ID biomarkers and stress erythropoiesis manifest. Two interventions were completed, each comprising a four-week baseline, a four-week intervention at either sea level or altitude, and a four-week follow-up. Participants (n=39) were randomly assigned to 20 IU·kg bw-1 rHuEPO or placebo injections every second day for three weeks during the two intervention periods. Venous blood was collected weekly. Altitude increased ERFE (P≤0.001) with no changes in hepcidin or routine iron biomarkers, making ERFE of clinical relevance as an early marker of moderate hypoxia. rHuEPO treatment at sea level induced a similar pattern of changes in ERFE (P<0.05) and hepcidin levels (P<0.05), demonstrating the impact of accelerated erythropoiesis and not of other hypoxia-induced mechanisms. Compared to altitude alone, concurrent rHuEPO treatment and altitude exposure induced additive changes in hepcidin (P<0.05) and ERFE (P≤0.001) parallel with increases in hematocrit (P<0.001), demonstrating a relevant range of both hepcidin and ERFE. A poor but significant correlation between hepcidin and ERFE was found (R2=0.13, P<0.001). The findings demonstrate that hepcidin and ERFE are more rapid biomarkers of changes in iron demands than routine iron markers. Finally, ERFE and hepcidin may be sensitive markers in an anti-doping context.

Effect of intermittent hypoxia on hematological parameters after recombinant human erythropoietin administration

2009 ◽  
Vol 107 (4) ◽  
pp. 429-436 ◽  
Author(s):  
F. Sanchis-Gomar ◽  
V. E. Martinez-Bello ◽  
E. Domenech ◽  
A. L. Nascimento ◽  
F. V. Pallardo ◽  
...  
Keyword(s):  
Recombinant Human Erythropoietin ◽  
Intermittent Hypoxia ◽  
Hematological Parameters ◽  
Human Erythropoietin

scholarly journals Self-sampling of capillary blood for serological testing of SARS-CoV-2 by COVID-19 IgG ELISA

2020 ◽  
Author(s):  
Lottie Brown ◽  
Rachel Louise Byrne ◽  
Alice Fraser ◽  
Sophie I Owen ◽  
Ana I Cubas Atienzar ◽  
...  
Keyword(s):  
Large Scale ◽  
Venous Blood ◽  
Dried Blood Spots ◽  
Capillary Blood ◽  
Blood Collection ◽  
Serological Testing ◽  
Perfect Agreement ◽  
Blood Samples ◽  
Large Scale Testing ◽  
Feasible Alternative

Serological testing is emerging as a powerful tool to progress our understanding of COVID-19 exposure, transmission and immune response. Large-scale testing is limited by the need for in-person blood collection by staff trained in venepuncture. Capillary blood self-sampling and postage to laboratories for analysis could provide a reliable alternative. Two-hundred and nine matched venous and capillary blood samples were obtained from thirty nine participants and analysed using a COVID-19 IgG ELISA to detect antibodies against SARS-CoV-2. Thirty seven out of thirty eight participants were able to self-collect an adequate sample of capillary blood (≥50 μl). Using plasma from venous blood collected in lithium heparin as the reference standard, matched capillary blood samples, collected in lithium heparin-treated tubes and on filter paper as dried blood spots, achieved a Cohen′s kappa coefficient of >0.88 (near-perfect agreement). Storage of capillary blood at room temperature for up to 7 days post sampling did not affect concordance. Our results indicate that capillary blood self-sampling is a reliable and feasible alternative to venepuncture for serological assessment in COVID-19.

Plasma Carnitine Profile during Chronic Renal Anemia Treatment with Recombinant Human Erythropoietin

2003 ◽  
Vol 26 (1) ◽  
pp. 33-38 ◽  
Author(s):  
A. Dębska-Ślizień ◽  
A. Owczarzak ◽  
D. Kunicka ◽  
W. Łysiak-Szydłowska ◽  
B. Rutkowski
Keyword(s):  
Recombinant Human Erythropoietin ◽  
Chronic Renal Disease ◽  
Iron Status ◽  
Hematological Parameters ◽  
Hemodialysis Patients ◽  
Maintenance Hemodialysis ◽  
Plasma Carnitine ◽  
Human Erythropoietin ◽  
Carnitine Metabolism ◽  
Hb Concentration

Recombinant human erythropoietin (epoetin) is widely used for correction of anaemia in patients with chronic renal disease and its efficacy has been confirmed in numerous studies. Disturbances in carnitine metabolism may also contribute to the development of renal anaemia. Although increases in erythrocyte count (RBC) and changes in RBC metabolism during L-carnitine administration have been observed, supplementation with L-carnitine in anaemic hemodialysis patients is not routine. The aim of our study was to determine the influence of epoetin on hematological parameters and plasma carnitine profile in anaemic hemodialysis patients. 36 hemodialysis patients (22 men, 14 female, aged from 17 to 64 years, mean 43) and 30 healthy volunteers (12 men, 18 female, aged from 25 to 65 years, mean 40) were studied. Epoetin (Eprex, Janssen-Cilag) was administered subcutaneously for twelve months with the starting dose 2000 IU three times per week (range from 75 to 133, mean 102±21 IU/kg/week). The target hemoglobin (Hb) range at the time of the study was between 10–11 g/dL. Laboratory markers of hematological response, carnitine and iron status, were measured before epoetin administration and then controlled every three months. During epoetin treatment a significant increase in Hb concentration was observed (100% of patients responded to epoetin). In the third and six month of epoetin treatment, along with a significant increase in mean reticulocyte count and the highest increment of RBC count and Hb levels, probably due to increased erythropoiesis, a significant, transient decrease of mean total and free plasma carnitine levels was observed. This may suggest the utilisation of carnitine by a new RBC population. It also indicates that there is a need for L-carnitine in carnitine deficient maintenance hemodialysis patients particularily during erythropoiesis induced by epoetin treatment.

scholarly journals Self-sampling of capillary blood for SARS-CoV-2 serology

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lottie Brown ◽  
Rachel L. Byrne ◽  
Alice Fraser ◽  
Sophie I. Owen ◽  
Ana I. Cubas-Atienzar ◽  
...  
Keyword(s):  
Large Scale ◽  
Venous Blood ◽  
Dried Blood Spots ◽  
Capillary Blood ◽  
Blood Collection ◽  
Perfect Agreement ◽  
Blood Samples ◽  
Large Scale Testing ◽  
Feasible Alternative ◽  
Lateral Flow Tests

AbstractSerological testing is emerging as a powerful tool to progress our understanding of COVID-19 exposure, transmission and immune response. Large-scale testing is limited by the need for in-person blood collection by staff trained in venepuncture, and the limited sensitivity of lateral flow tests. Capillary blood self-sampling and postage to laboratories for analysis could provide a reliable alternative. Two-hundred and nine matched venous and capillary blood samples were obtained from thirty nine participants and analysed using a COVID-19 IgG ELISA to detect antibodies against SARS-CoV-2. Thirty eight out of thirty nine participants were able to self-collect an adequate sample of capillary blood (≥ 50 µl). Using plasma from venous blood collected in lithium heparin as the reference standard, matched capillary blood samples, collected in lithium heparin-treated tubes and on filter paper as dried blood spots, achieved a Cohen’s kappa coefficient of > 0.88 (near-perfect agreement, 95% CI 0.738–1.000). Storage of capillary blood at room temperature for up to 7 days post sampling did not affect concordance. Our results indicate that capillary blood self-sampling is a reliable and feasible alternative to venepuncture for serological assessment in COVID-19.

scholarly journals Diagnostic Accuracy of Dried Blood Spots Collected on HemaSpot HF Devices Compared to Venous Blood Specimens To Estimate Measles and Rubella Seroprevalence

mSphere ◽  
2021 ◽  
Author(s):  
Christine Prosperi ◽  
Ojas Kaduskar ◽  
Vaishali Bhatt ◽  
Alvira Z. Hasan ◽  
Jeromie W. Vivian Thangaraj ◽  
...  
Keyword(s):  
Venous Blood ◽  
High Sensitivity ◽  
Dried Blood Spots ◽  
Blood Collection ◽  
Serum Samples ◽  
Community Based ◽  
Acceptable Alternative ◽  
Specific Immunoglobulin ◽  
Blood Specimens ◽  
Blood Spot

Dried blood spot (DBS) collection provides an easy, practical, and acceptable alternative to venous blood collection, especially for community-based studies, provided that results from DBS are accurate. We demonstrated high sensitivity and specificity for measles- and rubella-specific immunoglobulin G (IgG) with DBS collected via HemaSpot HF devices compared to serum samples.

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