Genetically Engineered Intracellular Single-Chain Antibodies in Gene Therapy

2002 ◽  
Vol 22 (2) ◽  
pp. 191-212 ◽  
Author(s):  
Guadalupe Bilbao ◽  
Juan Luis Contreras ◽  
David T Curiel
Keyword(s):  
Gene Therapy ◽  
Genetically Engineered ◽  
Single Chain ◽  
Single Chain Antibodies

scholarly journals Gene Therapy for Infectious Diseases

1998 ◽  
Vol 11 (1) ◽  
pp. 42-56 ◽  
Author(s):  
Bruce A. Bunnell ◽  
Richard A. Morgan
Keyword(s):  
Gene Therapy ◽  
Infectious Diseases ◽  
Infectious Agent ◽  
Catalytic Rna ◽  
Single Chain ◽  
Gene Therapies ◽  
Single Chain Antibodies ◽  
Wide Range ◽  
Management Approaches ◽  
I Gene

SUMMARY Gene therapy is being investigated as an alternative treatment for a wide range of infectious diseases that are not amenable to standard clinical management. Approaches to gene therapy for infectious diseases can be divided into three broad categories: (i) gene therapies based on nucleic acid moieties, including antisense DNA or RNA, RNA decoys, and catalytic RNA moieties (ribozymes); (ii) protein approaches such as transdominant negative proteins and single-chain antibodies; and (iii) immunotherapeutic approaches involving genetic vaccines or pathogen-specific lymphocytes. It is further possible that combinations of the aforementioned approaches will be used simultaneously to inhibit multiple stages of the life cycle of the infectious agent.

scholarly journals AAV2/8 Anti-angiogenic Gene Therapy Using Single-Chain Antibodies Inhibits Murine Choroidal Neovascularization

2019 ◽  
Vol 13 ◽  
pp. 86-98 ◽  
Author(s):  
Chris P. Hughes ◽  
Neil M.J. O’Flynn ◽  
Maureen Gatherer ◽  
Michelle E. McClements ◽  
Jennifer A. Scott ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Choroidal Neovascularization ◽  
Single Chain ◽  
Single Chain Antibodies ◽  
Angiogenic Gene

Intracellular Single-Chain Antibodies for Gene Therapy

2003 ◽  
pp. 121-149
Author(s):  
Guadalupe Bilbao ◽  
Jesus Gomez-Navarro ◽  
Keizo Kazano ◽  
Juan Luis Contreras ◽  
David T. Curiel
Keyword(s):  
Gene Therapy ◽  
Single Chain ◽  
Single Chain Antibodies

scholarly journals Identification of single chain antibodies to breast cancer stem cells using phage display

2009 ◽  
pp. NA-NA ◽  
Author(s):  
Deniz Gur ◽  
Suling Liu ◽  
Anurag Shukla ◽  
Stephanie C Pero ◽  
Max S Wicha ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Phage Display ◽  
Breast Cancer Stem Cells ◽  
Single Chain ◽  
Single Chain Antibodies

scholarly journals Efficient and Selective Gene Transfer into Primary Human Brain Tumors by Using Single-Chain Antibody-Targeted Adenoviral Vectors with Native Tropism Abolished

2002 ◽  
Vol 76 (6) ◽  
pp. 2753-2762 ◽  
Author(s):  
Victor W. van Beusechem ◽  
Jacques Grill ◽  
D. C. Jeroen Mastenbroek ◽  
Thomas J. Wickham ◽  
Peter W. Roelvink ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Gene Transfer ◽  
Brain Tumors ◽  
Human Brain ◽  
Cancer Gene Therapy ◽  
Adenoviral Vectors ◽  
Receptor Expression ◽  
Normal Brain ◽  
Cancer Gene ◽  
Single Chain

ABSTRACT The application of adenoviral vectors in cancer gene therapy is hampered by low receptor expression on tumor cells and high receptor expression on normal epithelial cells. Targeting adenoviral vectors toward tumor cells may improve cancer gene therapy procedures by providing augmented tumor transduction and decreased toxicity to normal tissues. Targeting requires both the complete abolition of native tropism and the addition of a new specific binding ligand onto the viral capsid. Here we accomplished this by using doubly ablated adenoviral vectors, lacking coxsackievirus-adenovirus receptor and αv integrin binding capacities, together with bispecific single-chain antibodies targeted toward human epidermal growth factor receptor (EGFR) or the epithelial cell adhesion molecule. These vectors efficiently and selectively targeted both alternative receptors on the surface of human cancer cells. Targeted doubly ablated adenoviral vectors were also very efficient and specific with primary human tumor specimens. With primary glioma cell cultures, EGFR targeting augmented the median gene transfer efficiency of doubly ablated adenoviral vectors 123-fold. Moreover, EGFR-targeted doubly ablated vectors were selective for human brain tumors versus the surrounding normal brain tissue. They transduced organotypic glioma and meningioma spheroids with efficiencies similar to those of native adenoviral vectors, while exhibiting greater-than-10-fold-reduced background levels on normal brain explants from the same patients. As a result, EGFR-targeted doubly ablated adenoviral vectors had a 5- to 38-fold-improved tumor-to-normal brain targeting index compared to native vectors. Hence, single-chain targeted doubly ablated adenoviral vectors are promising tools for cancer gene therapy. They should provide an improved therapeutic index with efficient tumor transduction and effective protection of normal tissue.

Optimization of the expression of single-chain antibodies using different Escherichia coli systems

2007 ◽  
Vol 131 (2) ◽  
pp. S251-S252
Author(s):  
Symon Riedstra ◽  
Gonçalo Leite ◽  
Carolina Ferreira ◽  
Francisco Brás Gomes ◽  
Paulo M.P. Costa ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Single Chain ◽  
Single Chain Antibodies
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