scholarly journals Identification and Quantification of Germacrene D from the Extracts of Zanthoxylum ovalifolium Leaves by AgNO3-TLC Densitometric Method

2019 ◽  
Vol 31 (8) ◽  
pp. 1649-1652
Author(s):  
B.S. Theerthavathy ◽  
Shaukath Ara Khanum ◽  
S. Ravi Kiran
Keyword(s):  
Linear Regression ◽  
Thin Layer ◽  
Linear Relationship ◽  
Correlation Coefficient ◽  
Mobile Phase ◽  
High Performance ◽  
Chromatographic Method ◽  
Analysis Data ◽  
Germacrene D ◽  
Simultaneous Identification

Germacrene D an important sesquiterpenoid has been extracted from leaves of Zanthoxylum ovalifoilum and high performance thin-layer chromatographic method has been developed for its simultaneous identification and determination. The compound was extracted by cold extraction and separated and identified on a AgNO3 impregnated silica gel 60 F254 HPTLC plates with dichloromethane:acetone (9.8:0.2 v/v) as mobile phase. The quantification of geremcare D was carried out by densitometry scanning performed at λ = 254 nm by reflectance scanning. This facilitated a well-resolved band for the compound with a Rf value of 0.46 ± 0.01. Linear regression concentration analysis data for mean calibration plots showed good linear relationship in the range of 1000-10000 ng/mL, with a correlation coefficient, slope and intercept of 0.9985 ± 0.0004, 21.62 ± 0.435 and 3308.87 ± 10.24, respectively. The minimum amount of germacrene D that could be authentically detected (LOD) and quantified (LOQ) were determined and found to be 212.07 and 521.23 ng/spot, respectively. The method was validated by considering the parameters linearity, precision, accuracy, specificity and robustness wherein, it is evident from the results that the proposed method was simple, safe and reliable.

scholarly journals Validated HPTLC Method for Quantification of Luteolin and Apigenin inPremna mucronataRoxb., Verbenaceae

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Nayan G. Patel ◽  
Kalpana G. Patel ◽  
Kirti V. Patel ◽  
Tejal R. Gandhi
Keyword(s):  
Thin Layer ◽  
Mobile Phase ◽  
High Performance ◽  
Chromatographic Method ◽  
Methanolic Extract ◽  
Limit Of Detection ◽  
Quantitative Estimation ◽  
Reflection Mode ◽  
Limit Of Quantitation ◽  
Hptlc Method

A simple, rapid, and precise high-performance thin-layer chromatographic method was developed for quantitative estimation of luteolin and apigenin inPremna mucronataRoxb., family Verbenaceae. Separation was performed on silica gel 60 F254HPTLC plates using toluene : ethyl acetate : formic acid (6 : 4 : 0.3) as mobile phase for elution of markers from extract. The determination was carried out in fluorescence mode using densitometric absorbance-reflection mode at 366 nm for both luteolin and apigenin. The methanolic extract ofPremna mucronatawas found to contain 10.2 mg/g % luteolin and 0.165 mg/g % of apigenin. The method was validated in terms of linearity, LOD and LOQ, accuracy, precision, and specificity. The calibration curve was found to be linear between 200 and 1000 ng/band for luteolin and 50 and 250 ng/band for apigenin. For luteolin and apigenin, the limit of detection was found to be 42.6 ng/band and 7.97 ng/band while the limit of quantitation was found to be 129.08 ng/band and 24.155 ng/band, respectively. This developed validated method is capable of quantifying and resolving luteolin and apigenin and can be applicable for routine analysis of extract and plant as a whole.

scholarly journals IDENTIFICATION, QUANTIFICATION AND VALIDATION OF STIGMASTEROL FROM ALPINIA CALCARATA USING HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY METHOD

2017 ◽  
Vol 9 (9) ◽  
pp. 126
Author(s):  
Pratheema Philomindoss
Keyword(s):  
Linear Regression ◽  
Thin Layer ◽  
Linear Relationship ◽  
High Performance ◽  
Retention Factor ◽  
Chromatography Method ◽  
Ich Guidelines ◽  
Layer Chromatography ◽  
Hptlc Method

Objective: The present study is designed to develop a new simple, precise, rapid and selective high‐performance thin‐layer chromatographic (HPTLC) method for the determination of stigmasterol in methanolic rhizomes extract of Alpinia calcarata.Methods: As per International Conference on Harmonization (ICH) guidelines we have applied different concentrations of stigmasterol as standard on HPTLC plates for the quantification of stigmasterol from the Alpinia calcarata rhizomes. The concentration of standard stigmasterol is 1 mg/ml.Results: The retention factor of stigmasterol was 0.58. Linearity was obtained in the range of 50 ng‐250 ng for stigmasterol. The developed and validated HPTLC method was employed for stigmasterol in methanolic rhizomes extract of Alpinia calcarata for standardization of the content of the marker. The linear regression data for the calibration plots showed a good linear relationship with r=0.99977 for stigmasterol, respectively Satisfactory recoveries of 99.77 % were obtained for stigmasterol.Conclusion: The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of stigmasterol in methanolic rhizomes extract of Alpinia calcarata

scholarly journals Simultaneous analysis of eprosartan and hydrochlorothiazide in tablet formulation by High- Performance thin layer chromatography with ultraviolet absorption densitometry

2021 ◽  
Vol 8 (3) ◽  
pp. 123-128
Author(s):  
Vrushali Tambe ◽  
Vijaya Vichare ◽  
Anupa Wagh ◽  
Surekha Wavhal
Keyword(s):  
Thin Layer ◽  
Mobile Phase ◽  
High Performance ◽  
Chromatographic Method ◽  
Glacial Acetic Acid ◽  
Tablet Formulation ◽  
Simultaneous Analysis ◽  
Phase Detection ◽  
Retention Factors ◽  
Layer Chromatography

A new, simple, accurate, and precise high-performance thin-layer chromatographic method has been established for simultaneous analysis of Eprosartan and Hydrochlorothiazide from a tablet formulation. Standard and sample solutions of Eprosartan and Hydrochlorothiazide were applied to precoated 250 μm layer of silica gel G 60 F and the plates were developed with Chloroform: Acetonitrile: Glacial Acetic Acid (7:3:1,v/v/v) as mobile phase. Detection and evaluation of densitograms was performed densitometrically at 254 nm. The linear range was 200-700 ng/band with the retention factors of Eprosartan and Hydrochlorothiazide were 0.26± 0.02 and 0.44±0.02, respectively. The method was validated and successfully used for analysis of the drugs in tablets.

scholarly journals Assay of Clopidogrel by Using HPLTC Method

2018 ◽  
pp. 557-561
Author(s):  
D. S. Ghotekar ◽  
Vishal N. Kushare
Keyword(s):  
Regression Analysis ◽  
Linear Regression ◽  
Thin Layer ◽  
Stationary Phase ◽  
High Performance ◽  
Linear Regression Analysis ◽  
Analysis Data ◽  
Solvent System ◽  
Assay Method ◽  
Layer Chromatography

The present paper describes stability indicating high-performance thin-layer chromatography (HPTLC) assay method for clopidogrel in bulk drugs. The method employed TLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. The solvent system consisted of toluene: methanol: triethylamine (6.5: 4.0: 0.1 v/v/v). The system was found to give compact spot for clopidogrel (<em>R</em><sub>f</sub>value of 0.40 ± 0.010). Densitometric analysis of Clopidogrel was carried out in the absorbance mode at 254 nm. The linear regression analysis data for the calibration plots showed good linear relationship with <em>r</em>2 = 0.888 with respect to peak area in the concentration range 30 - 120 ng/spot.

scholarly journals HPTLC METHOD FOR THE DETERMINATION OF LUPEOL FROM ANDROGRAPHIS ECHIOIDES LEAVES

2018 ◽  
Vol 10 (5) ◽  
pp. 102
Author(s):  
S. Gurupriya ◽  
L. Cathrine ◽  
P. Pratheema
Keyword(s):  
Linear Regression ◽  
Thin Layer ◽  
Linear Relationship ◽  
High Performance ◽  
Retention Factor ◽  
International Conference ◽  
Good Linear ◽  
Ich Guidelines ◽  
Hptlc Method

Objective: A new simple, precise, rapid and selective high‐performance thin‐layer chromatographic (HPTLC) method has been developed for the determination of lupeol in methanolic leaves extract of Andrographis echioides.Methods: As per International Conference on Harmonization (ICH) guidelines we have applied different concentrations of lupeol as standard on HPTLC plates for the quantification of lupeol from the Andrographis echioides leaves. The concentration of standard lupeol was 1 mg/ml.Results: The retention factor of lupeol was found to be 0.55. Linearity was obtained in the range of 5000 ng‐10000 ng for lupeol. The developed and validated HPTLC method was employed for lupeol in methanolic leaves extract of Andrographis echioides for standardization of the content of the marker. The linear regression data for the calibration plots showed a good linear relationship with r=0.99917 for lupeol. Satisfactory recoveries of 99.80 % were obtained for lupeol.Conclusion: The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of lupeol in methanolic leaves extract of Andrographis echioides.

Validated high-performance thin-layer chromatographic method for the determination of dibenzyl cyclooctadiene lignans fromSchisandra grandiflora

2014 ◽  
Vol 27 (6) ◽  
pp. 460-465 ◽  
Author(s):  
Katragunta Kumar ◽  
Bokka Ramesh ◽  
Vadaparthi Rao ◽  
Borra Poornima ◽  
Vanka Sarma ◽  
...  
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Chromatographic Method

scholarly journals Stability indicating thin-layer chromatographic method for estimation of antidiabetic drug Remogliflozin etabonate

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Dimal A. Shah ◽  
Ishita I. Gondalia ◽  
Vandana B. Patel ◽  
Ashok Mahajan ◽  
Usmangani Chhalotiya ◽  
...  
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Chromatographic Method ◽  
Tablet Formulation ◽  
Base Hydrolysis ◽  
Forced Degradation ◽  
Stability Indicating ◽  
Forced Degradation Studies ◽  
Degradation Studies ◽  
Remogliflozin Etabonate

Abstract Background A sensitive, precise, and stability-indicating high-performance thin-layer chromatographic (HPTLC) method has been developed for the analysis of Remogliflozin etabonate in tablet formulation. HPTLC plates precoated with silica gel 60 F254 were used as the stationary phase; methanol: ethyl acetate: toluene: NH3 (2:4:4:0.1, v/v/v) was used as mobile phase, and densitometry was used for the quantitative estimation of the drug. The proposed method was validated with respect to linearity, accuracy, precision, and robustness and applied for the estimation of drug in tablet dosage form. Results The Rf value of Remogliflozin etabonate was observed to be 0.61. The densitometric estimation was performed in reflectance mode at 229 nm. The method was found to be linear in the range of 500–8000 ng/band for Remogliflozin etabonate. The possible degradation pathway was estimated by performing forced degradation studies. The degradant peaks were well resolved from the drug peak with acceptable resolution in their Rf value. Conclusion An accurate and precise high-performance thin-layer chromatographic method has been developed for the quantification of Remogliflozin etabonate in tablets. Forced degradation studies were performed, and drug was found to be highly susceptible to acid, base hydrolysis, and oxidative stress degradation and gets converted into active drug Remogliflozin. Both Remogliflozin etabonate and Remogliflozin bands were well resolved. The method was applied for the analysis of drug in tablet formulation, and it can be used for routine quality control analysis, as well as for the analysis of stability samples.

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