scholarly journals Interaction of Midazolam with Glassy Carbon Supported Lipid Membrane in the Presence and Absence of Marker Ions

2021 ◽  
Vol 33 (5) ◽  
pp. 994-1000
Author(s):  
M. Padmapriya ◽  
K. Parvathi ◽  
S. Rameshkumar

A biomimetic membrane was formed on the surface of electrochemically activated glassy carbon electrode in NaCl bath solutions. The variation of electrochemical properties of solid supported bilayer lipid membrane (s-BLM) with NaCl concentration in the bath solutions in the presence and absence of ferri/ferrocyanide marker ions was discussed using electrochemical impedance spectroscopy. The extent of pore formation on the s-BLM surface was discussed using bode phase diagram. The electrochemical impedance studies show that the partition of midazolam into the s-BLM strongly depends on Cl– ion concentration in the bath solutions. The variation of membrane capacitance with drug dose shows the ionized form of midazolam interaction with the surface of s-BLM while the neutral and ion pair forms get partitioned into the membrane. In the presence of marker ions, the membrane resistance increases with decrease in NaCl concentration in the bath solution. The cyclic voltammetric responses of marker ions for bare and drug doped s-BLMs in NaCl bath solutions were recorded and variation of redox peak currents with drug dose was discussed.

2019 ◽  
Vol 31 (5) ◽  
pp. 1169-1175
Author(s):  
S. Sivakumar ◽  
S. Rameshkumar ◽  
S. Sathishkumar ◽  
A. Sankar

Artificial planar bilayer phospholipid membrane was formed in KCl bath solutions and its electrical properties have been analyzed using electrochemical impedance spectroscopy. The stability of planar bilayer lipid membrane depends on concentration of KCl in the bath. Changes in the electrical properties of planar bilayer lipid membrane with the addition of lorazepam were also studied using electrochemical impedance spectroscopy. Lorazepam gets partitioned into bilayer lipid membrane and exhibited a fluidization effect. The ionic conductance across the bilayer lipid membrane increased with the addition of lorazepam to the bath. An impedimetric sensor was developed for quantification of lorazepam in solution.


2014 ◽  
Vol 16 (20) ◽  
pp. 9546-9555 ◽  
Author(s):  
Jacek Kozuch ◽  
Conrad Weichbrodt ◽  
Diego Millo ◽  
Karin Giller ◽  
Stefan Becker ◽  
...  

SEIRA spectroscopy provides insight into the potential-induced structural changes of the anion channel hVDAC1 embedded in the tethered bilayer lipid membrane.


2020 ◽  
Vol 59 (SI) ◽  
pp. SIIK02
Author(s):  
Yasutaka Tomioka ◽  
Shogo Takashima ◽  
Masataka Moriya ◽  
Hiroshi Shimada ◽  
Fumihiko Hirose ◽  
...  

Membranes ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 11
Author(s):  
Damian Dziubak ◽  
Kamil Strzelak ◽  
Slawomir Sek

Supported lipid membranes are widely used platforms which serve as simplified models of cell membranes. Among numerous methods used for preparation of planar lipid films, self-assembly of bicelles appears to be promising strategy. Therefore, in this paper we have examined the mechanism of formation and the electrochemical properties of lipid films deposited onto thioglucose-modified gold electrodes from bicellar mixtures. It was found that adsorption of the bicelles occurs by replacement of interfacial water and it leads to formation of a double bilayer structure on the electrode surface. The resulting lipid assembly contains numerous defects and pinholes which affect the permeability of the membrane for ions and water. Significant improvement in morphology and electrochemical characteristics is achieved upon freeze–thaw treatment of the deposited membrane. The lipid assembly is rearranged to single bilayer configuration with locally occurring patches of the second bilayer, and the number of pinholes is substantially decreased. Electrochemical characterization of the lipid membrane after freeze–thaw treatment demonstrated that its permeability for ions and water is significantly reduced, which was manifested by the relatively high value of the membrane resistance.


Micromachines ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 53
Author(s):  
Nobuo Misawa ◽  
Satoshi Fujii ◽  
Koki Kamiya ◽  
Toshihisa Osaki ◽  
Shoji Takeuchi

This paper describes a method for a bilayer lipid membrane (BLM) formation using a perforated sheet along with an open chamber. Microscopic observation of the formed membrane showed a typical droplet interface bilayer. We proved that the formed membrane was a BLM based on electrical measurements of the membrane protein α-hemolysin, which produces nanopores in BLMs. Unlike the conventional approach for BLM formation based on the droplet contact method, this method provides aqueous surfaces with no organic solvent coating layer. Hence, this method is suitable for producing BLMs that facilitate the direct addition of chemicals into the aqueous phase.


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