scholarly journals Molecular biology as a diagnostic tool for detection of Leptospira spp. in cows of a border region – case report

2021 ◽  
Vol 37 ◽  
pp. e37067
Author(s):  
Giovani Batista Pastre ◽  
Isabela Carvalho Santos ◽  
Lidiane Nunes Barbosa ◽  
Edinalva Almeida Mota ◽  
Roberta Torres Chideroli ◽  
...  

The reproductive efficiency of livestock is the basis for the success of livestock, dairy or beef, and having high reproductive performance depends on several factors within the production system and the presence of infectious diseases of the reproductive sphere in the herd is one of the factors that can compromise that efficiency. The aim of this study was to use molecular biology as a diagnostic tool for the detection of Leptospira spp. DNA in cows with reproductive disorders on a rural property in the municipality of Boca do Acre, Amazonas, Brazil. Vaginal mucus was collected from nine Nelore breeding cows with a history of abortion and birth of weak calves submitted to DNA extraction and nested-PCR technique for 16S gene amplification at the bacterial genus level. Of the nine samples analyzed, five (55.55%) amplified a product of 331bp. The municipality of Boca do Acre is bordered by Peru and Bolivia, and knowledge of the prevalence of the disease, serovars, and circulating Leptospira species is essential for the adoption of measures related to animal husbandry, as well as health education for ranchers and their workers to avoid a possible occupational infection since this disease is considered an important zoonosis. New molecular studies using primers that allow the identification of the Leptospira species and mainly pathogenic species should be conducted in this region in order to elucidate the possible species of this etiological agent and the possible reservoirs of the disease to begin the understanding of the epidemiology of this disease in cattle in this region of border.

Science ◽  
1970 ◽  
Vol 170 (3955) ◽  
pp. 349-351 ◽  
Author(s):  
J. T. Edsall
Keyword(s):  

2013 ◽  
Vol 88 (1) ◽  
pp. 128-129 ◽  
Author(s):  
Paulo Ricardo Criado ◽  
Lívia Delgado ◽  
Gustavo Alonso Pereira

Dermoscopy has being used over the past twenty years as a noninvasive aid in the diagnosis of innumerable skin conditions, including infectious diseases and infestations (Entodermoscopy).Tinea nigra is a superficial phaeohyfomycosis that affects mainly the glabrous skin of palms and soles. We describe a 14 year-old girl with a three-month history of an enlarging brown patch of her hand diagnosed as Tinea Nigra following clinical and dermoscopy examination.These images emphasize the importance of dermoscopy as a diagnostic tool in the daily routine of dermatologists.


2021 ◽  
Author(s):  
◽  
Janine Ruth Cook

<p>Within the New Zealand poultry industry press between 1900 and 1960, scientific approaches were promoted and ‘sentimentality’ discouraged, yet comparative and anthropomorphic description suggesting similarities between chickens and humans persisted. Feathered Friends and Human Animals explores this phenomenon within poultry journals, newspapers, advice books and official publications. Four key themes of comparison are identified: ideas about the chicken mind, the chicken-as-worker, poultry ‘eugenics’, and health and hygiene.  It is argued that humanitarian, theological, and philosophical ideas, the ‘natural’ empathetic and humoured identification that arises through everyday contact with animals within relatively small systems, and the rationalisation of industry, were all significant factors contributing to sustained comparison. However, the public articulation of fundamental biological ideas – encapsulated in the modern, overarching concept of ‘general biology’ – validated and integrated these discourses.  General biology influenced new trends in education and in the popular and public articulation of research into the life sciences of this period. It encouraged the integration of sympathetic naturalist persepectives, including evolutionary based ideas about ‘natural laws’, with emerging new science that continued to establish many fundamental biological principles through extrapolation from experimental animals to human animals. This study demonstrates that poultry experts’ attended to this same blend of older naturalist science and new scientific knowledge.  Historians’ focus on emerging specialist science in the early twentieth century has tended to obfuscate the realities of science education within the applied sciences and amongst lay audiences, and the continued interest in fundamental aspects of biology within professional science. The findings of this study reveal that farming ideas did not develop within a bubble, determined only by animal husbandry traditions and industry-specific applied research. They also suggest that practitioners’ conceptions of biology within applied fields of this era were not as distinct as has been supposed.  As a ‘bottom-up’ cultural history of science, this study illustrates the articulation of general biology within an agricultural context. This is the key contribution offered to local and international historiography. However, other elements of the study expand existing scholarship. In exploring ideas about race and eugenics, it offers a broader framework for social historians, who, while cognisant of the eugenic mind-set of this period, have granted little attention to general biology as a professional trend. It offers insight into the agendas and tensions within school nature study and elementary science. It is also the first comprehensive history of the New Zealand poultry industry. Poultry-keeping engaged up to around 60 percent of the nation’s households in this period, including thousands of farmers who kept sideline flocks, but as a predominantly domestic (as opposed to export) industry it has been overlooked by social and agricultural historians.  The field of human animal studies, which has tended to gloss over both this era of transition prior to modern agribusiness and scientific discourses, is also advanced by this study, and this is the first New Zealand agricultural history to engage with this field and examine animal husbandry ideologically. It reveals how fundamental science knowledge, entwined with moral perspectives, continued to shape ideas about animals’ needs and behaviour well beyond the Victorian period. Assumptions of similarity however, were not always beneficial for the animal, and human-bird comparison was used to both justify and deny kind treatment.</p>


2021 ◽  
Vol 901 (1) ◽  
pp. 012040
Author(s):  
A V Sidorenko ◽  
G F Yartsev ◽  
R K Baikasenov ◽  
T P Aysuvakova ◽  
B B Karta-bayeva ◽  
...  

Abstract Agriculture continues to move forward confidently. Before our eyes, the agrarian industry is reviving, becoming stronger, taking a strong position at the forefront of the Russian economy. The most important achievement of 2017 was a record grain harvest of 130 million tons. This result was achieved not only by increasing the cultivated areas, which increased by 620 thousand hectares in 2017, but also thanks to the record yield, which amounted to 28 kg / ha, which is 80% higher than in 2000. The maximum harvest in the history of new and Soviet Russia, which will ensure our country a strong leadership in the world wheat market. Russian agriculture remains a driver of the country’s economic development. For the second year in a row, we get a record harvest of not only grain, but also sugar beets and sunflowers, the production of soybeans, rapeseed and greenhouse vegetables is growing. In the new season, grain exports will reach 45 million tons, including more than 35 million tons of wheat. Russia is expanding the geography of its presence, having mastered new directions for the supply of agricultural products. At the end of 10 months of 2017, grain exports increased by 22% compared to the same period in 2016 and amounted to 32 million tons. During the same time, the export of Russian wheat exceeded 24 million tons, which is 23% more than a year earlier. … In Russia, over 17 years, the volume of grain production increased 2 times, sugar beet and sunflower - 2-3 times, soybeans and rapeseed - 10, greenhouse vegetables - 1.8, poultry meat - 6, pork - 2. Fish - 2 times. Greenhouse vegetable growing and horticulture are developing intensively. Progress is being made in the development of animal husbandry and aquaculture. The dairy industry is undergoing a significant transformation, and production volumes on farms are growing. These are new growth points for the agro-industrial complex. Thanks to the achievements of domestic scientists, grain production of agricultural crops can be increased due to many factors. One of the factors is foliar dressing with liquid micro- and macro-fertilizers. Therefore, we studied various combinations of liquid micro-, macrofertilizers, as well as the bio-logical product Albit, in order to identify the best option and recommend it for produc-tion.


2021 ◽  
Vol 8 (12) ◽  
pp. 304
Author(s):  
Ivana Piredda ◽  
Loris Bertoldi ◽  
Giuseppe Benvenuto ◽  
Bruna Palmas ◽  
Aureliana Pedditzi ◽  
...  

Aim of this study was to evaluate, the presence and diversity of Leptospira spp. in blood and urine samples collected from 175 owned-dogs from Sardinia, Italy. After determination of leptospiral infection by microscopic agglutination test (MAT), urine from MAT-positive dogs were examined by real-time polymerase chain reaction (lipL32 rt-PCR) and then isolated by culture. In order to characterize obtained serovars, positive cultures were then subjected to 16S rRNA and secY sequencing, phylogenetic analysis and Multilocus Sequence Typing (MLST). Results showed that seven dogs (4%; 95% CI: 0–55) had Leptospira DNAs in their urine and five strains were isolated from urine cultures. The three different sequence types (ST17, ST198 and ST24) belonging to Leptospira interrogans genomospecies identified by MLST analyses in this study, confirmed that the leptospiral infection was widespread in Sardinian dogs. We also reported the first characterization of a new Leptospira spp. isolated from urine of one dog living in the study area. Whole genome sequencing and phylogenetic analysis, confirmed that this genospecies was closely related to Leptospira hovindhougenii, an intermediate Leptospira spp. with unknown pathogenicity previously isolated from a rat in Denmark. Further studies are required to clarify whether healthy dogs that shed leptospires in their urine could represent a zoonotic risk for humans in this region.


2012 ◽  
Vol 53 (3) ◽  
pp. 301-323 ◽  
Author(s):  
WALTER E. A. VAN BEEK

ABSTRACTA rare document, the diary of a slave raider, offers a unique view into the sociopolitical situation at the turn of the nineteenth century in the colonial backwater of North Cameroon. The Fulbe chief in question, Hamman Yaji, not only kept a diary, but was by far the most notorious slave raider of the Mandara Mountains. This article supplements the data from his diary with oral histories and archival sources to follow the dynamics of the intense slave raiding he engaged in. This frenzy of slaving occurred in a ‘colonial interstice’ characterized by competition between three colonial powers – the British, the Germans and the French, resilient governing structures in a region poorly controlled by colonial powers, and the unclear boundaries of the Mandara Mountains. The dynamics of military technology and the economics of this ‘uncommon market’ in slaves form additional factors in this episode in the history of slavery in Africa. These factors account for the general situation of insecurity due to slave raiding in the area, to which Hamman Yaji was an exceptionally atrocious contributor. In the end a religious movement, Mahdism, stimulated the consolidation of colonial power, ending Yaji's regime, which in all its brutality provides surprising insight in the early colonial situation in this border region between Nigeria and Cameroon.


2019 ◽  
pp. 1-44
Author(s):  
Angma Dey Jhala

During the eighteenth century, the travelogue flourished as a genre and was used to describe peoples both familiar and unfamiliar to the western observer. Chapter 1 examines one such account, the 1798 travelogue of the Scottish doctor Francis Buchanan in the CHT. In his tour diary, he deployed the language of natural history to describe not only the region’s unusual soil quality, topography, and local jhum or swidden agriculture, but also the religious, cultural, and linguistic practices of the various hill tribes he encountered. In the process, he exposed the tumultuous history of this border region, which found itself at the crossroads of imperial ambition by both the East India Company and the kingdom of Burma. He is also an intriguing example of an Enlightenment era man of science and reason in the Chittagong Hill Tracts.


2021 ◽  
pp. 1-20
Author(s):  
Faisal H. Husain

This chapter introduces the themes and arguments of the book. In particular, it explains the benefits of adopting a holistic approach to the history of the river basin that acknowledges its cultural, physical, and biological unity. Treating the Tigris and Euphrates as a continuous whole brings to light the magnitude and significance of river flow that fostered contacts between upstream and downstream regions. Beyond facilitating communication, the twin rivers formed the backbone of the early modern Ottoman economy in the region by supporting complementary subsistence strategies, such as irrigation agriculture, animal husbandry, and wetland exploitation. In addition to the themes and arguments, this chapter offers a brief introduction to the history of the Ottoman Empire and the ecology of the Tigris-Euphrates basin.


Author(s):  
Richard A. Schatz ◽  
Phillip P. Toskes

Case History—A 78 yr old woman presenting with a five-year history of diarrhoea. Updates Pathogenesis—discussion of the possible role of proton pump inhibitors. Diagnosis—comparison of lactulose hydrogen, glucose hydrogen, and 14C D-xylose breath tests; preliminary studies of circulating CdtB antibodies as a diagnostic tool....


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