scholarly journals Experimental infection model with the bivalvulid Enteromyxum leei (Myxidiidae) in the sharpsnout seabream, Diplodus puntazzo (Sparidae), and evaluation of the antiparasitic efficacy of a functional diet

2020 ◽  
Vol 67 ◽  
Author(s):  
Morgane A. Henry ◽  
Fotini Kokou ◽  
Oswaldo Palenzuela ◽  
George Pyrenis ◽  
George Rigos
Keyword(s):  
Experimental Infection ◽  
Infection Model ◽  
Diplodus Puntazzo ◽  
Sharpsnout Seabream ◽  
Experimental Infection Model ◽  
Enteromyxum Leei

A pasture-based experimental infection model for footrot in sheep

2020 ◽  
pp. 106305
Author(s):  
Andrew S. McPherson ◽  
Richard J. Whittington ◽  
Ruth M. Kennan ◽  
Julian I. Rood ◽  
Om P. Dhungyel
Keyword(s):  
Experimental Infection ◽  
Infection Model ◽  
Experimental Infection Model

scholarly journals Whip-LAMP: a novel LAMP assay for the detection of Trichuris muris-derived DNA in stool and urine samples in a murine experimental infection model

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Pedro Fernández-Soto ◽  
Carlos Fernández-Medina ◽  
Susana Cruz-Fernández ◽  
Beatriz Crego-Vicente ◽  
Begoña Febrer-Sendra ◽  
...  
Keyword(s):  
Molecular Diagnosis ◽  
Experimental Infection ◽  
Lamp Assay ◽  
Diagnostic Methods ◽  
Urine Samples ◽  
Infection Model ◽  
Trichuris Muris ◽  
Stool Samples ◽  
First Time ◽  
Experimental Infection Model

Abstract Background Trichuris trichiura (human whipworm) infects an estimated 477 million individuals worldwide. In addition to T. trichiura, other Trichuris species can cause an uncommon zoonosis and a number of human cases have been reported. The diagnosis of trichuriasis has relied traditionally on microscopy. Recently, there is an effort to use molecular diagnostic methods, mainly qPCR. LAMP technology could be an alternative for qPCR especially in low-income endemic areas. Trichuris muris, the causative agent of trichuriasis in mice, is of great importance as a model for human trichuriasis. Here, we evaluate the diagnostic utility of a new LAMP assay in an active experimental mouse trichuriasis in parallel with parasitological method by using stool and, for the first time, urine samples. Methods Stool and urine samples were collected from mice infected with eggs of T. muris. The dynamics of infection was determined by counting the number of eggs per gram of faeces. A LAMP based on the 18S rRNA gene from T. muris was designed. Sensitivity and specificity of LAMP was tested and compared with PCR. Stool and urine samples were analysed by both LAMP and PCR techniques. Results Trichuris muris eggs were detected for the first time in faeces 35 days post-infection. LAMP resulted specific and no cross-reactions were found when using 18 DNA samples from different parasites. The detection limit of the LAMP assay was 2 pg of T. muris DNA. When testing stool samples by LAMP we obtained positive results on day 35 p.i. and urine samples showed amplification results on day 20 p.i., i.e. 15 days before the onset of T. muris eggs in faeces. Conclusions To the best of our knowledge, we report, for the first time, a novel LAMP assay (Whip-LAMP) for sensitive detection of T. muris DNA in both stool and urine samples in a well-established mice experimental infection model. Considering the advantages of urine in molecular diagnosis in comparison to stool samples, should make us consider the possibility of starting the use urine specimens in molecular diagnosis and for field-based studies of human trichuriasis where possible. Further studies with clinical samples are still needed.

scholarly journals Experimental infection model for Sin Nombre hantavirus in the deer mouse (Peromyscus maniculatus)

2000 ◽  
Vol 97 (19) ◽  
pp. 10578-10583 ◽  
Author(s):  
J. Botten ◽  
K. Mirowsky ◽  
D. Kusewitt ◽  
M. Bharadwaj ◽  
J. Yee ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Peromyscus Maniculatus ◽  
Deer Mouse ◽  
Infection Model ◽  
Experimental Infection Model

scholarly journals Establishment of a novel tick-Babesia experimental infection model

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Hiroki Maeda ◽  
Takeshi Hatta ◽  
M Abdul Alim ◽  
Daigo Tsubokawa ◽  
Fusako Mikami ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Infection Model ◽  
Experimental Infection Model

scholarly journals Cynomolgus Monkeys (Macaca fascicularis) as an Experimental Infection Model for Human Group A Rotavirus

Viruses ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 355 ◽  
Author(s):  
Gentil Bentes ◽  
Juliana Guimarães ◽  
Eduardo Volotão ◽  
Alexandre Fialho ◽  
Cleber Hooper ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Macaca Fascicularis ◽  
Infection Model ◽  
Human Group ◽  
Cynomolgus Monkeys ◽  
Group A Rotavirus ◽  
Group A ◽  
Experimental Infection Model

Establishment and comparison of Actinobacillus pleuropneumoniae experimental infection model in mice and piglets

2019 ◽  
Vol 128 ◽  
pp. 381-389 ◽  
Author(s):  
Chun-tong Bao ◽  
Jia-meng Xiao ◽  
Bai-jun Liu ◽  
Jian-fang Liu ◽  
Ri-ning Zhu ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Actinobacillus Pleuropneumoniae ◽  
Infection Model ◽  
Experimental Infection Model

Efficacy of commercial and field-strain Mycobacterium paratuberculosis vaccinations with recombinant IL-12 in a bovine experimental infection model

Vaccine ◽  
2003 ◽  
Vol 21 (23) ◽  
pp. 3101-3109 ◽  
Author(s):  
Jude E. Uzonna ◽  
Paula Chilton ◽  
Robert H. Whitlock ◽  
Perry L. Habecker ◽  
Phillip Scott ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Field Strain ◽  
Infection Model ◽  
Mycobacterium Paratuberculosis ◽  
Experimental Infection Model

Magnetic resonance imaging in the evaluation of inflammatory lesions in muscular and soft tissues: an experimental infection model induced by Candida albicans

1999 ◽  
Vol 17 (9) ◽  
pp. 1327-1334 ◽  
Author(s):  
J Ruiz–Cabello ◽  
B Carrero–González ◽  
P Avilés ◽  
C Santisteban ◽  
R.J Méndez ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Candida Albicans ◽  
Magnetic Resonance ◽  
Experimental Infection ◽  
Soft Tissues ◽  
Infection Model ◽  
Resonance Imaging ◽  
Inflammatory Lesions ◽  
Experimental Infection Model

scholarly journals An experimental infection model to induce digital dermatitis infection in cattle

2012 ◽  
Vol 95 (4) ◽  
pp. 1821-1830 ◽  
Author(s):  
A. Gomez ◽  
N.B. Cook ◽  
N.D. Bernardoni ◽  
J. Rieman ◽  
A.F. Dusick ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Infection Model ◽  
Digital Dermatitis ◽  
Experimental Infection Model
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