Differential Influence of Imidazolium Ionic Liquid on Cellulase Kinetics in Saccharification of Cellulose and Lignocellulosic Biomass Substrate

2021 ◽  
Author(s):  
Prapakorn Tantayotai ◽  
Marttin Paulraj Gundupalli ◽  
Elizabeth Jayex Panakkal ◽  
Malinee Sriariyanun ◽  
Kittipong Rattanaporn ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Rice Straw ◽  
Process Optimization ◽  
Critical Concentration ◽  
Enzymatic Saccharification ◽  
Imidazolium Ionic Liquid ◽  
One Pot ◽  
Cellulase Kinetics ◽  
Positive Effect ◽  
Insight Into

The effect of [Emim][OAc] on Celluclast 1.5 L, Accellerase 1500, and IL-tolerant (MSL2) cellulase during the saccharification of carboxymethylcellulose (CMC), Avicel (AV), rice straw (RS) was studied in one pot process (pretreatment and saccharification). The inhibition caused by [Emim][OAc] (0.5–2.0 M) with substrate loading (20–50 mg/mL) were also evaluated. In most cases, the inhibition mode of saccharification for CMC and AV was identified to be uncompetitive inhibition when the concentration of [Emim][OAc] was higher than 0.5 M. Under the influence of 0.5 M of [Emim][OAc], the Critical Concentration of Substrate (CCS) values of the Celluclast 1.5 L and Accellerase 1500 on CMC hydrolysis were determined to be at 26.59 and 33.65 mg/mL, respectively. Also, increasing in [Emim][OAc] concentration could increase in CCS values, suggesting the positive effect of [Emim][OAc] on the improvement of enzymatic saccharification. This study provides insight into the process optimization for integration of [Emim][OAc] in one pot process of biorefinery.

scholarly journals One-Pot Ionic Liquid-Mediated Bioprocess for Pretreatment and Enzymatic Hydrolysis of Rice Straw with Ionic Liquid-Tolerance Bacterial Cellulase

2022 ◽  
Vol 9 (1) ◽  
pp. 17
Author(s):  
Malinee Sriariyanun ◽  
Nichaphat Kitiborwornkul ◽  
Prapakorn Tantayotai ◽  
Kittipong Rattanaporn ◽  
Pau-Loke Show
Keyword(s):  
Ionic Liquid ◽  
Saline Soil ◽  
Enzymatic Saccharification ◽  
Value Added ◽  
Specific Enzyme ◽  
One Pot ◽  
The One ◽  
Hydrolysis Of ◽  
Bacterial Cellulase ◽  
Value Added Products

Ionic liquid (IL) pretreatment of lignocellulose is an efficient method for the enhancement of enzymatic saccharification. However, the remaining residues of ILs deactivate cellulase, therefore making intensive biomass washing after pretreatment necessary. This study aimed to develop the one-pot process combining IL pretreatment and enzymatic saccharification by using low-toxic choline acetate ([Ch][OAc]) and IL-tolerant bacterial cellulases. Crude cellulases produced from saline soil inhabited Bacillus sp. CBD2 and Brevibacillus sp. CBD3 were tested under the influence of 0.5–2.0 M [Ch][OAc], which showed that their activities retained at more than 95%. However, [Ch][OAc] had toxicity to CBD2 and CBD3 cultures, in which only 32.85% and 12.88% were alive at 0.5 M [Ch][OAc]. Based on the specific enzyme activities, the sugar amounts produced from one-pot processes using 1 mg of CBD2 and CBD3 were higher than that of Celluclast 1.5 L by 2.0 and 4.5 times, respectively, suggesting their potential for further application in the biorefining process of value-added products.

scholarly journals Enzymatic Saccharification of Rice Straw under Influence of Recycled Ionic Liquid Pretreatments

2016 ◽  
Vol 100 ◽  
pp. 160-165 ◽  
Author(s):  
Kraipat Cheenkachorn ◽  
Tom Douzou ◽  
Supacharee Roddecha ◽  
Prapakorn Tantayotai ◽  
Malinee Sriariyanun
Keyword(s):  
Ionic Liquid ◽  
Rice Straw ◽  
Enzymatic Saccharification

Chlorination of Aromatics with Trichloroisocyanuric Acid (TCICA) in Brønsted-Acidic Imidazolium Ionic Liquid [BMIM(SO3H)][OTf]: an Economical, Green Protocol for the Synthesis of Chloroarenes

2007 ◽  
Vol 60 (12) ◽  
pp. 923 ◽  
Author(s):  
Abigail Hubbard ◽  
Takao Okazaki ◽  
Kenneth K. Laali
Keyword(s):  
Density Functional Theory ◽  
Ionic Liquid ◽  
Density Functional ◽  
Survey Study ◽  
Imidazolium Ionic Liquid ◽  
Functional Theory ◽  
Green Protocol ◽  
Mild Conditions ◽  
Trichloroisocyanuric Acid ◽  
Insight Into

A survey study on electrophilic chlorination of aromatics with trichloroisocyanuric acid (TCICA) in Brønsted-acidic imidazolium ionic liquid [BMIM(SO3H)][OTf] is reported. The reactions are performed under very mild conditions (at ~50°C) and give good to excellent yields, depending on the substrates. Chemoselectivity for mono- v. dichlorination can be tuned by changing the arene-to-TCICA ratio and the reaction time. The survey study and competitive experiments suggest that triprotonated/protosolvated TCICA is a selective/moderately reactive transfer-chlorination electrophile. Density functional theory was used as guide to obtain further insight into the nature of the chlorination electrophile and the transfer-chlorination step.

Insight into the reversible behavior of Lewis-Brønsted basic poly(ionic liquid)s in one-pot two-step chemical fixation of CO2 to linear carbonates

2021 ◽  
Author(s):  
Yuting He ◽  
Huimin Lu ◽  
Xue Li ◽  
Jun Wu ◽  
Tiancheng Pu ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Green Chemistry ◽  
Chemical Fixation ◽  
One Pot ◽  
Basic Catalysts ◽  
Poly Ionic Liquid ◽  
Insight Into

The multi-step reaction of CO2 over basic catalysts significantly improves the diversity of products, vividly embodying the ability of green chemistry to turn waste into treasure. However, under a Lewis...

NMR and DFT Insight into the Synergistic Role of Bovine Serum Albumin-Ionic Liquid for Multicomponent Cascade Aldol/Knoevenagel-thia-Michael/Michael Reactions in One Pot

ChemCatChem ◽  
2016 ◽  
Vol 8 (19) ◽  
pp. 3050-3056 ◽  
Author(s):  
Yogesh Thopate ◽  
Richa Singh ◽  
Arun K. Sinha ◽  
Vikash Kumar ◽  
Mohammad Imran Siddiqi
Keyword(s):  
Ionic Liquid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
One Pot ◽  
Michael Reactions ◽  
Insight Into

Easily measurable pH as an indicator of the effectiveness of the aqueous cholinium ionic liquid-based pretreatment of lignocellulose

RSC Advances ◽  
2014 ◽  
Vol 4 (98) ◽  
pp. 55635-55639 ◽  
Author(s):  
Yan-Xia An ◽  
Ning Li ◽  
Min-Hua Zong ◽  
Wen-Yong Lou
Keyword(s):  
Ionic Liquid ◽  
Rice Straw ◽  
Strong Correlation ◽  
Enzymatic Saccharification

Thirty-nine aqueous cholinium IL solutions were used to pretreat rice straw for improving the enzymatic saccharification. A strong correlation between the pH of aqueous IL solutions and their pretreatment effectiveness was observed.

One-pot production of lactic acid from rice straw pretreated with ionic liquid

2021 ◽  
Vol 323 ◽  
pp. 124563
Author(s):  
Neerja Yadav ◽  
Lata Nain ◽  
Sunil K. Khare
Keyword(s):  
Ionic Liquid ◽  
Lactic Acid ◽  
Rice Straw ◽  
One Pot

Unexpected self-assembly, photoluminescence behavior, and film-forming properties of polysiloxane-based imidazolium ionic liquids prepared by one-pot thiol–ene reaction

2017 ◽  
Vol 41 (23) ◽  
pp. 14545-14550 ◽  
Author(s):  
Yujing Zuo ◽  
Zhiming Gou ◽  
Zhaoyue Li ◽  
Jinwan Qi ◽  
Shengyu Feng
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Self Assembly ◽  
High Yield ◽  
Imidazolium Ionic Liquid ◽  
One Pot ◽  
Ene Reaction ◽  
Imidazolium Ionic Liquids ◽  
Film Forming ◽  
Photoluminescence Behavior

The synthesis of a series of polysiloxane-based ionic liquids (PNLs) is reported via straightforward thiol–ene reaction with high yield using three kinds of imidazolium ionic liquid monomers.

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