Powder microscopic, physicochemical and chromatographic approach for the quality control of anti-hypertensive drug Rattha Piththathirku Kudinir Chooranam

The present work aims to study powder microscopy, physicochemical and high-performance thin-layer chromatography photo documentation and fingerprint profiles of a Siddha drug, Rattha Piththathirku Kudinir Chooranam (RPK). The raw drugs were collected, authenticated and the RPK was prepared. Then the drug was investigated for powder microscopic characters, physicochemical parameters, Thin Layer Chromatographic photo documentation (TLC), High-Performance Thin-Layer Chromatographic (HPTLC) fingerprint profiles of successive n-hexane, successive chloroform, successive ethanol and hydro alcohol (1:1) extracts. The successive and hydro alcohol extracts of the drug displayed distinct TLC spots and HPTLC peaks which are distinct to this drug.

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Quality control in a centralized reconstitution unit of cytotoxic agents: Determination of etoposide using high-performance thin-layer chromatography

Journal of Oncology Pharmacy Practice ◽

10.1177/107815529600200105 ◽

1996 ◽

Vol 2 (1) ◽

pp. 35-41

Author(s):

Cécile M. Beaupin ◽

Roland Darmanaden

Keyword(s):

Quality Control ◽

Thin Layer ◽

Thin Layer Chromatography ◽

High Performance ◽

Cytotoxic Agents ◽

Layer Chromatography

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Comparison of Antioxidant Activity and Main Active Compounds Among Different Parts of Alpinia officinarum Hance Using High-Performance Thin Layer Chromatography-Bioautography

Journal of AOAC International ◽

10.5740/jaoacint.18-0307 ◽

2019 ◽

Vol 102 (3) ◽

pp. 726-733 ◽

Cited By ~ 2

Author(s):

Wan-Xin Zhang ◽

In-Cheng Chao ◽

De-jun Hu ◽

Farid Shakerian ◽

Liya Ge ◽

...

Keyword(s):

Antioxidant Activity ◽

Quality Control ◽

Thin Layer ◽

Thin Layer Chromatography ◽

High Performance ◽

Aerial Parts ◽

Alpinia Officinarum ◽

Layer Chromatography ◽

Different Parts ◽

Tof Ms

Abstract Background: Alpinia officinarum Hance (ginger family) is an important Chinese medicine, especially in Southern China. Objective: A simple and effective high-performance thin-layer chromatography coupled with 2, 2-diphenyl-1-picrylhydrazyl bioautography (HPTLC-DPPH) and electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS) method was developed for the bioactivity-based quality control of A. officinarum. Methods: The HPTLC-DPPH and ESI-Q-TOF-MS/MS were applied for the analysis of different parts of A. officinarum after using methanol extraction for 23 batches of taproot, four batches of aerial, and three batches of fibril parts. Results: The systematic evaluation showed that similar components in taproot and aerial parts make the major antioxidant activity. However, based on our evaluation, the antioxidant ability of the aerial parts is lower than the taproot parts. There is also a significant difference (P < 0.05) between taproot and fibril parts of the root. The chemical structures of compounds with the antioxidant capacity were tentatively identified as 5R-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone (band 1), kaempferide (band 2), and galangin (band 3) based on ESI-Q-TOF-MS/MS analytical results and further confirmed by standards. Conclusions: This identification indicated that two flavonoid compounds and one diarylheptanoid compound possessed high potentials to be used as the antioxidant biomarkers for the quality control of A. officinarum. Highlights: The comparison of different parts could be considered as guidelines for the usage of A. officinarum.

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