scholarly journals A novel murine model of reversible bile duct obstruction demonstrates rapid improvement of cholestatic liver injury

2020 ◽  
Vol 8 (10) ◽  
Author(s):  
Sarah A. Taylor ◽  
Xin‐Yi Yeap ◽  
Jiao‐Jing Wang ◽  
Kyle D. Gromer ◽  
Alyssa Kriegermeier ◽  
...  
Keyword(s):  
Bile Duct ◽  
Liver Injury ◽  
Murine Model ◽  
Bile Duct Obstruction ◽  
Rapid Improvement ◽  
Duct Obstruction ◽  
Cholestatic Liver Injury

Nitric Oxide-mediated Liver Injury in the Presence of Experimental Bile Duct Obstruction

2003 ◽  
Vol 27 (3) ◽  
pp. 253-255 ◽  
Author(s):  
Atilla Engin ◽  
B. Şükrü Bozkurt ◽  
Nilgün Altan ◽  
Leyla Memiş ◽  
Neslihan Bukan
Keyword(s):  
Nitric Oxide ◽  
Bile Duct ◽  
Liver Injury ◽  
Bile Duct Obstruction ◽  
Duct Obstruction

scholarly journals Effect of Rotavirus Strain on the Murine Model of Biliary Atresia

2006 ◽  
Vol 81 (4) ◽  
pp. 1671-1679 ◽  
Author(s):  
Steven R. Allen ◽  
Mubeen Jafri ◽  
Bryan Donnelly ◽  
Monica McNeal ◽  
David Witte ◽  
...  
Keyword(s):  
Bile Duct ◽  
Epithelial Cell ◽  
Biliary Atresia ◽  
Murine Model ◽  
Clinical Signs ◽  
Bile Duct Obstruction ◽  
Biliary Epithelial Cell ◽  
Newborn Mice ◽  
Live Virus ◽  
Duct Obstruction

ABSTRACT Biliary atresia is a devastating disorder of the newborn in which afflicted infants develop inflammation and fibrosis of the extrahepatic biliary tract, resulting in cirrhosis and end-stage liver disease. Infection with a virus is thought to be a contributing factor in the etiology of biliary atresia. In the murine model of biliary atresia, perinatal exposure to rhesus rotavirus (RRV) results in biliary epithelial cell infection causing bile duct obstruction. The purpose of this study was to determine if tropism for the biliary epithelial cell was unique to RRV. Newborn mice underwent intraperitoneal injection with five strains of rotavirus: RRV (simian), SA11-FM (simian/bovine), SA11-SM (simian), EDIM (murine), and Wa (human). RRV and SA11-FM caused clinical manifestations of bile duct obstruction and high mortality. SA11-SM caused clinical signs of hepatobiliary injury but the mortality was markedly reduced. EDIM and Wa caused no sign of hepatobiliary disease. The systemic and temporal distribution of viral protein and live virus varied according to the injected strain. Immunohistochemistry revealed that RRV and SA11-FM targeted the biliary epithelial cells. In contrast, SA11-SM was found in the liver but in not in the biliary epithelium. These results indicate that strain-specific characteristics dictate tropism for cells of hepatobiliary origin which in turn impact the ability to induce the murine model of biliary atresia.

Exercise in rats with liver injury induced by carbon tetrachloride or bile duct obstruction. Blood cholic acid and liver histology

1982 ◽  
Vol 181 (2) ◽  
pp. 135-145 ◽  
Author(s):  
V. Simko ◽  
R. E. Kelley ◽  
H. P. Dincsoy ◽  
Z. Gimmon ◽  
K. Willmann
Keyword(s):  
Carbon Tetrachloride ◽  
Bile Duct ◽  
Liver Injury ◽  
Cholic Acid ◽  
Liver Histology ◽  
Bile Duct Obstruction ◽  
Duct Obstruction

Bid-mediated apoptosis does not contribute to cholestatic liver injury following bile duct ligation

2009 ◽  
Vol 47 (01) ◽  
Author(s):  
P Nalapareddy ◽  
S Schüngel ◽  
MP Manns ◽  
H Jaeschke ◽  
A Vogel
Keyword(s):  
Bile Duct ◽  
Liver Injury ◽  
Bile Duct Ligation ◽  
Duct Ligation ◽  
Cholestatic Liver Injury

Fibrolamellar carcinoma as a cause of bile duct obstruction

Pathology ◽  
1988 ◽  
Vol 20 (4) ◽  
pp. 326-331 ◽  
Author(s):  
Robert P. Eckstein ◽  
Christopher P. Bambach ◽  
Daniel Stiel ◽  
James Roche ◽  
Benjamin N. Goodman
Keyword(s):  
Bile Duct ◽  
Bile Duct Obstruction ◽  
Duct Obstruction ◽  
Fibrolamellar Carcinoma

scholarly journals Feasibility and Safety of Endoluminal Radiofrequency Ablation as a Rescue Treatment for Bilateral Metal Stent Obstruction Due to Tumor Ingrowth in the Hilum: A Pilot Study

2021 ◽  
Vol 10 (5) ◽  
pp. 952
Author(s):  
Hoonsub So ◽  
Chi Hyuk Oh ◽  
Tae Jun Song ◽  
Hyun Woo Lee ◽  
Jun Seong Hwang ◽  
...  
Keyword(s):  
Radiofrequency Ablation ◽  
Bile Duct ◽  
Adverse Events ◽  
Bile Duct Obstruction ◽  
Metal Stents ◽  
Metal Stent ◽  
Duct Obstruction ◽  
Tumor Ingrowth ◽  
Malignant Bile Duct Obstruction ◽  
Hilar Bile Duct

Background. Radiofrequency ablation (RFA) is a palliative method known for its application in the endoscopic treatment of malignant bile duct obstruction. It may be a useful rescue method for metal stent malfunction caused by tumor ingrowth. This study aimed to examine the feasibility and safety of endoluminal RFA for occluded bilateral hilar metal stents due to tumor ingrowth in patients with malignant hilar bile duct obstruction. Methods: From March 2016 to June 2018, 11 patients with unresectable malignant hilar bile duct stricture with occluded bilateral hilar metal stents due to tumor ingrowth were enrolled. Endoluminal RFA was performed through a novel temperature-controlled catheter at a setting of 7 W power for 120 s with a target temperature of 80 °C via endoscopic retrograde cholangiopancreatography (ERCP). The patients’ demographics, clinical outcomes, and adverse events were investigated. Results: The median age was 64 (interquartile range, 54–72) years. All RFA procedures were successful. Clinical success was achieved in eight patients (72.7%). During the follow-up, eight patients (72.7%) showed stent dysfunction, and the median patency after RFA was 50 days (95% confidence interval (CI): 34–not available (NA)). All stent dysfunctions were successfully managed with ERCP. Ten patients died, and the median overall survival was 289 days (95% CI, 107–NA) from RFA to death. There was one case of mild abdominal pain after the procedure without serious adverse events. Conclusions: As a rescue therapy for occluded bilateral hilar metal stents due to tumor ingrowth, endoluminal RFA seemed to be safe and useful in selected patients.

scholarly journals Keratin 7 expression in hepatic cholestatic diseases

2021 ◽  
Author(s):  
S. Sakellariou ◽  
C. Michaelides ◽  
T. Voulgaris ◽  
J. Vlachogiannakos ◽  
E. Manesis ◽  
...  
Keyword(s):  
Bile Duct ◽  
Inverse Correlation ◽  
Bile Duct Obstruction ◽  
Hepatocyte Proliferation ◽  
Primary Biliary Cholangitis ◽  
Specific Marker ◽  
Ductular Reaction ◽  
Duct Obstruction ◽  
Cholestatic Diseases ◽  
Keratin 7

AbstractWe evaluated keratin 7 (K7) hepatocellular expression in 92 patients with common types of acute and chronic cholestatic diseases caused by bile duct obstruction/destruction or parenchymal lesions [acute hepatitis (n=20), mixed/pure cholestasis (n=16), primary biliary cholangitis-PBC (n=35), primary sclerosing cholangitis-PSC (n=10), vanishing bile duct syndrome (n=3), complete large bile duct obstruction due to space-occupying lesions (n=8)]. K7 immunohistochemical hepatocellular expression and ductular reaction (DR) were semi-quantitatively assessed. Results were correlated with liver enzyme serum levels, cholestasis type, histological features, hepatocellular Ki67 labelling index (LI) and HepPar1 expression. Hepatocellular K7 expression was detected in 87% (81/92) cases and in all cholestatic disease types with lowest incidence in pure/mixed cholestasis and highest in incomplete bile duct obstruction (iBDO), reaching 100% in PSC. K7-positive hepatocytes had low Ki67 LI (0-5%) retaining HepPar1 expression, irrespective of disease type. PSC cases had high K7 hepatocellular expression even with intact bile ducts, a feature that may aid differential diagnosis of cholestatic syndromes. K7 hepatocellular expression significantly correlated with cholestasis type, bile duct loss and fibrosis stage. It was higher in milder acute cholestatic hepatitis showing inverse correlation with hepatocyte proliferation and serum transaminase levels. In iBDO, younger age independently correlated with high K7 expression, while serum GGT levels showed a nearly significant correlation. Correlation with DR findings implied that K7-positive hepatocytes may result through metaplasia. In conclusion, K7 hepatocellular expression is a sensitive though non-specific marker of cholestasis. It may represent a cytoprotective reaction of resting hepatocytes in cholestasis of longer duration especially in younger patients.

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