scholarly journals Evidence for abnormal linkage between urine oxalate and citrate excretion in human kidney stone formers

2021 ◽  
Vol 9 (13) ◽  
Author(s):  
Megan L. Prochaska ◽  
Orson W. Moe ◽  
John R. Asplin ◽  
Fredric L. Coe ◽  
Elaine M. Worcester
Keyword(s):  
Kidney Stone ◽  
Human Kidney ◽  
Stone Formers ◽  
Urine Oxalate

2087 CRYSTAL KINETICS AND PROCESSING IN HUMAN KIDNEY STONE FORMATION: COMPARISON OF CLINICAL AND PATHOLOGICAL FINDINGS IN STONE FORMERS AND NON-STONE FORMERS

2012 ◽  
Vol 187 (4S) ◽  
Author(s):  
Atsushi Okada ◽  
Takahiro Yasui ◽  
Kazumi Taguchi ◽  
Kazuhiro Niimi ◽  
Yasuhiro Fujii ◽  
...  
Keyword(s):  
Kidney Stone ◽  
Stone Formation ◽  
Human Kidney ◽  
Pathological Findings ◽  
Stone Formers ◽  
Kidney Stone Formation

scholarly journals Excretion of urine extracellular vesicles bearing markers of activated immune cells and calcium/phosphorus physiology differ between calcium kidney stone formers and non-stone formers

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Jiqing Zhang ◽  
Sanjay Kumar ◽  
Muthuvel Jayachandran ◽  
Loren P. Herrera Hernandez ◽  
Stanley Wang ◽  
...  
Keyword(s):  
Urinary Excretion ◽  
Calcium Oxalate ◽  
Extracellular Vesicles ◽  
Immune Cells ◽  
Kidney Stone ◽  
Fibroblast Growth Factor 23 ◽  
Pathogenic Mechanisms ◽  
Phosphorus Excretion ◽  
Stone Formers ◽  
Calcium Phosphorus

Abstract Backgrounds: Previous studies have demonstrated that excretion of urinary extracellular vesicles (EVs) from different nephron segments differs between kidney stone formers and non-stone formers (NSFs), and could reflect pathogenic mechanisms of urinary stone disease. In this study we quantified selected populations of specific urinary EVs carrying protein markers of immune cells and calcium/phosphorus physiology in calcium oxalate stone formers (CSFs) compared to non-stone formers (NSFs). Methods Biobanked urine samples from CSFs (n = 24) undergoing stone removal surgery and age- and sex- matched NSFs (n = 21) were studied. Urinary EVs carrying proteins related to renal calcium/phosphorus physiology (phosphorus transporters (PiT1 and PiT2), Klotho, and fibroblast growth factor 23 (FGF23); markers associated with EV generation (anoctamin-4 (ANO4) and Huntington interacting protein 1 (HIP1)), and markers shed from activated immune cells were quantified by standardized and published method of digital flow cytometry. Results Urine excretion of calcium, oxalate, phosphorus, and calcium oxalate supersaturation (SS) were significantly higher in CSFs compared to NSFs (P < 0.05). Urinary excretion of EVs with markers of total leukocytes (CD45), neutrophils (CD15), macrophages (CD68), Klotho, FGF23, PiT1, PiT2, and ANO4 were each markedly lower in CSFs than NSFs (P < 0.05) whereas excretion of those with markers of monocytes (CD14), T-Lymphocytes (CD3), B-Lymphocytes (CD19), plasma cells (CD138 plus CD319 positive) were not different between the groups. Urinary excretion of EVs expressing PiT1 and PiT2 negatively (P < 0.05) correlated with urinary phosphorus excretion, whereas excretion of EVs expressing FGF23 negatively (P < 0.05) correlated with both urinary calcium and phosphorus excretion. Urinary EVs with markers of HIP1 and ANO4 correlated negatively (P < 0.05) with clinical stone events and basement membrane calcifications on papillary tip biopsies. Conclusions Urinary excretion of EVs derived from specific types of activated immune cells and EVs with proteins related to calcium/phosphorus regulation differed between CSFs and NSFs. Further validation of these and other populations of urinary EVs in larger cohort could identify biomarkers that elucidate novel pathogenic mechanisms of calcium stone formation in specific subsets of patients.

scholarly journals LOCALIZATION OF UROMUCOID IN HUMAN KIDNEY AND IN SECTIONS OF HUMAN KIDNEY STONE WITH THE FLUORESCENT ANTIBODY TECHNIQUE

1965 ◽  
Vol 13 (3) ◽  
pp. 155-160 ◽  
Author(s):  
H. J. KEUTEL
Keyword(s):  
Kidney Stones ◽  
Kidney Stone ◽  
Fluorescent Antibody ◽  
Human Kidney ◽  
Fluorescent Antibody Technique ◽  
Renal Tubules ◽  
Antibody Technique ◽  
The Matrix ◽  
Normal Human ◽  
The Common

Fluorescent labeled antibodies were used for the demonstration of uromucoid. This urine specific mucoprotein is demonstrably present only in the epithelial cells of the proximal segments of the normal human renal tubules and in the matrix of human kidney stones of all the common crystalline compositions.

scholarly journals Stone Composition Among First-Time Symptomatic Kidney Stone Formers in the Community

2015 ◽  
Vol 90 (10) ◽  
pp. 1356-1365 ◽  
Author(s):  
Prince Singh ◽  
Felicity T. Enders ◽  
Lisa E. Vaughan ◽  
Eric J. Bergstralh ◽  
John J. Knoedler ◽  
...  
Keyword(s):  
Kidney Stone ◽  
Stone Composition ◽  
Stone Formers ◽  
First Time

scholarly journals MP82-07 PRIMARY HYPERPARATHYROIDISM: CHALLENGES IN SCREENING AND DIAGNOSIS IN KIDNEY STONE FORMERS

2016 ◽  
Vol 195 (4S) ◽  
Author(s):  
Leslee Matheny ◽  
Tracy Marien ◽  
Mustafa Kadihasanoglu ◽  
Nicole L. Miller
Keyword(s):  
Primary Hyperparathyroidism ◽  
Kidney Stone ◽  
Stone Formers ◽  
Screening And Diagnosis

Risk of metabolic syndrome in kidney stone formers: A comparative cohort study

2021 ◽  
Vol 79 ◽  
pp. S341-S342
Author(s):  
R. Geraghty ◽  
E. Brain ◽  
P. Cook ◽  
P. Roderick ◽  
B.K. Somani
Keyword(s):  
Metabolic Syndrome ◽  
Cohort Study ◽  
Kidney Stone ◽  
Stone Formers ◽  
Comparative Cohort Study

Experimental Evaluation of Human Kidney Stone Spectra for Intraoperative Stone-Tissue-Instrument Analysis Using Autofluorescence

2019 ◽  
Vol 201 (1) ◽  
pp. 182-188 ◽  
Author(s):  
Jan Schütz ◽  
Arkadiusz Miernik ◽  
Albrecht Brandenburg ◽  
Daniel Schlager
Keyword(s):  
Experimental Evaluation ◽  
Kidney Stone ◽  
Human Kidney ◽  
Instrument Analysis

Gene expression of prothrombin in the human kidney and its potential relevance to kidney stone disease

1998 ◽  
Vol 81 (5) ◽  
pp. 666-672 ◽  
Author(s):  
Stapleton ◽  
Timme ◽  
Ryall
Keyword(s):  
Gene Expression ◽  
Kidney Stone ◽  
Human Kidney ◽  
Stone Disease ◽  
Kidney Stone Disease
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