Open circular holes with a certain size in beam web at 1/6 span is a new method to improve the seismic behavior of steel moment frames. Based on the results of elasticity theory, analytical model of frame with opening web is proposed, and analysis and verification with nonlinear finite element method (FEM) are conducted. Results show that, beam with opening web at 1/6 span can significantly reduce the axial stress of beam flange near the beam-column connections. It is helpful to achieve the design criterion of strong connection and weak member, and also to simplify the connection construction. If the diameter of web opening is appropriate, beam plastic hinges can move inward to beam section at opening web, and has little effect on bearing capacity, stiffness, energy dissipation capacity and ductility of frames. The method of web openings and the size range are presented at the end of this paper.