scholarly journals Failure to Detect Brown Adipose Tissue Uncoupling Protein mRNA in Benign Symmetric Lipomatosis (Madelung's Disease).

1994 ◽  
Vol 41 (3) ◽  
pp. 315-318 ◽  
Author(s):  
TSUTOMU KAZUMI ◽  
DANIEL RICQUIER ◽  
TETSUO MAEDA ◽  
TADAYUKI MASUDA ◽  
TOSHIKI HOZUMI ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Uncoupling Protein ◽  
Benign Symmetric Lipomatosis ◽  
Madelung's Disease ◽  
Madelung’S Disease ◽  
Brown Adipose ◽  
Uncoupling Protein Mrna

Selective loss of uncoupling protein mRNA in brown adipose tissue on deacclimation of cold-acclimated mice

1987 ◽  
Vol 65 (11) ◽  
pp. 955-959 ◽  
Author(s):  
Hasmukh V. Patel ◽  
Karl B. Freeman ◽  
Michel Desautels
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Cytochrome C Oxidase ◽  
Time Course ◽  
Uncoupling Protein ◽  
Relative Proportion ◽  
Cdna Probe ◽  
Mitochondrial Content ◽  
Brown Adipose ◽  
Uncoupling Protein Mrna

The time course of changes in the level of uncoupling protein mRNA when cold-acclimated mice were returned to a thermoneutral environment (33 °C) was examined using a cDNA probe. Upon deacclimation, there was a marked loss of uncoupling protein mRNA within 24 h, which precedes the loss of uncoupling protein from mitochondria. This loss of uncoupling protein mRNA was selective, since there was no change in the relative proportion of cytochrome c oxidase subunit IV mRNA or poly(A)+ RNA in total RNA. The results suggest that the decrease in the mitochondrial content of uncoupling protein during deacclimation is likely the result of turnover of existing protein, with very little replacement due to a lower level of its mRNA.

Effects of exposure temperature on brown adipose tissue uncoupling protein mRNA levels

1989 ◽  
Vol 67 (2-3) ◽  
pp. 147-151 ◽  
Author(s):  
Karl B. Freeman ◽  
Michael Heffernan ◽  
Zenobia Dhalla ◽  
Hasmukh V. Patel
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Uncoupling Protein ◽  
Effect Of Temperature ◽  
Initial Time ◽  
Maximal Response ◽  
Mrna Levels ◽  
Exposure Temperature ◽  
Brown Adipose ◽  
Uncoupling Protein Mrna

The effect of temperature on the amount of uncoupling protein mRNA in rat brown adipose tissue was examined after 1 and 14 days of exposure to cold. The relative amounts after 1 day, compared with rats kept at a thermoneutral temperature of 28 °C, were 3.2 at 19 °C, 3.3 at 11 °C, and 2.1 at 3 °C. This suggests that in warm-acclimated rats, a maximal response to a cold stimulus in brown adipose tissue is reached by 19 °C. In contrast to these results, the relative amounts of uncoupling protein mRNA after 14 days of cold exposure, compared with rats left at 28 °C, were 1.2 at 19 °C, 1.9 at 11 °C, and 2.1 at 3 °C. Since it is known that the amount of uncoupling protein in cold-acclimated rats increases continuously with decrease in temperature, the amount of protein reflects the mRNA levels during later times but not the initial time of exposure to cold.Key words: brown adipose tissue, uncoupling protein mRNA.

Loss of brown adipose tissue uncoupling protein mRNA on deacclimation of cold-exposed rats

1987 ◽  
Vol 142 (3) ◽  
pp. 696-701 ◽  
Author(s):  
Susanna Reichling ◽  
Robert G. Ridley ◽  
Hasmukh V. Patel ◽  
Calvin B. Harley ◽  
Karl B. Freeman
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Uncoupling Protein ◽  
Brown Adipose ◽  
Uncoupling Protein Mrna

Immunological detection of cDNA clones encoding the uncoupling protein of brown adipose tissue: Evidence for an antigenic determinant within the C-terminal eleven amino acids

1986 ◽  
Vol 6 (1) ◽  
pp. 87-94 ◽  
Author(s):  
Robert G. Ridley ◽  
Hasmukh V. Patel ◽  
Craig L. J. Parfett ◽  
Kimberly A. Olynyk ◽  
Susanna Reichling ◽  
...  
Keyword(s):  
Amino Acids ◽  
Adipose Tissue ◽  
Amino Acid ◽  
Brown Adipose Tissue ◽  
Antigenic Determinant ◽  
Uncoupling Protein ◽  
Cdna Clones ◽  
Amino Acid Homology ◽  
Brown Adipose ◽  
Uncoupling Protein Mrna

Poly(A)+ RNA was isolated from brown adipose tissue of cold acclimated rats and a fraction enriched for uncoupling protein mRNA was used to generate a cDNA library in pBR 322, Immunological screening of 1,500 colonies with an affinity-purified antiserum against the uncoupling protein yielded five positive clones, pUCPratl–5. Clone pUCPrat2 encoded the C-terminal 54 amino acids of rat uncoupling protein and exhibited 90% amino acid homology with the hamster protein. Clones pUCPrat3–5 encoded only the C-terminal 11 amino acids suggesting that an antigenic determinant lies within this sequence.

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