scholarly journals A Rapid Protocol for Isolating Genomic DNA from Tropical Grass Species Suitable for RAPD, ISSR and STS

CYTOLOGIA ◽  
2007 ◽  
Vol 72 (4) ◽  
pp. 427-434 ◽  
Author(s):  
Amaresh Chandra ◽  
Raghvendra Saxena
1981 ◽  
Vol 97 (1) ◽  
pp. 69-74 ◽  
Author(s):  
R. A. McKenzie ◽  
B. J. Blaney ◽  
R. J. W. Gartner

SUMMARYNegative calcium and phosphorus balances were produced in two experiments on six horses fed diets containing 2·6 or 4·3% total oxalate. The main calcium and phosphorus loss was in faeces. The urinary loss of calcium was reduced and that of phosphorus increased. A positive balance of magnesium was maintained. No clinically significant changes in blood concentrations of calcium, phosphorus, magnesium or alkaline phosphatase were produced. The bulk of ingested oxalate was unaccounted for in faeces and urine. It is suggested that the intestinal bacterial flora utilized the oxalate. The results supported the hypothesis that dietary oxalate is the cause of equine nutritional secondary hyperparathyroidism which arises when horses graze several tropical grass species.


Author(s):  
G. O. S. Ojo ◽  
H. Madu ◽  
A. I. Okwori

Seven improved tropical grass species, namely, Andropogon gayanus Kunth., Brachiaria ruziziensis Germain & Evrard, Cenchrus ciliaris L., Chloris gayana Kunth, Panicum maximum var. coloratum C.T, Paspalum orbiculare G. Forstand Sorghum almum Parodi were evaluated for yield and yield components at the Teaching and Research Farm of the Federal University of Agriculture, Makurdi, Nigeria in 2015 and 2016. The experiment was laid out in a randomized complete block design with three replications in each of the years. Results showed that the evaluated grass species could be cut at an early age when the leaf: stem ratio is high enough for the ruminants to derive maximum nutritional benefit and need not be delayed till 16 weeks after planting. The highest correlation between plant height and grain yield was observed at 4 weeks (r = 0.69) and 8 weeks (r = 0.70) after planting, implying that the taller the plant species at this stage of growth, the higher the probability for high grain yield. Identification and selection of tropical grass species for improvement in grain production should, therefore, be carried out between 4 and 8 weeks of growth. All the seven tropical forage grass species performed favourably well in terms of growth and yield, with the positive response to increasing rainfall and further studies on multi-location evaluation within the Southern Guinea Savanna agro-ecologicalal zone of Nigeria is recommended.


2012 ◽  
Vol 28 (3) ◽  
pp. 409-418 ◽  
Author(s):  
J. WANYAMA ◽  
K. HERREMANS ◽  
W. MAETENS ◽  
M. ISABIRYE ◽  
F. KAHIMBA ◽  
...  

2018 ◽  
Vol 40 (1) ◽  
pp. 1 ◽  
Author(s):  
C. J. L. du Toit ◽  
W. A. van Niekerk ◽  
H. H. Meissner ◽  
L. J. Erasmus ◽  
L. Morey

The development of greenhouse gas mitigation strategies has become an important issue globally. Enteric methane (CH4) emissions from livestock do not only contribute substantially to the environmental footprint of livestock production but it also represents a loss of energy that could be channelled towards animal growth and production. In this study 14 sub-tropical grass species typical of transitional rangeland regions of South Africa were characterised in terms of ecological status, chemical composition, in vitro total gas and CH4 production. The aim of the study was 2-fold: to identify grass species that could be selected for low enteric CH4 production; evaluate the influence of rangeland ecological status on the methanogenic potential of a rangeland. Grass samples were collected by hand, air-dried, milled and analysed for nutrient composition, in vitro organic matter digestibility (IVOMD) and in vitro gas and CH4 production. Cenchrus ciliaris and Urelytrum agropyriodes produced the highest 48-h in vitro CH4 of 17.49 and 14.05 mL/g DM digested respectively. The lowest 48-h in vitro CH4 was produced by Andropogan gayanus and Bothriochloa bladhii with 5.98 and 6.08 mL/g DM digested respectively. The evaluated grass species were overall of poor quality with low CP concentrations ranging from 2.4% for Trachypogon spicatus to 6.7% for Digitaria eriantha and IVOMD ranging from 22.5% for Andropogon gayanus to 42.2% for Urelytrum agropyriodes. Decreaser grass species presented with higher in vitro CH4 production compared with Increaser I and Increaser II grass species in the present study. The results of the study emphasise the importance of including the nutritional potential of grass species for improved livestock production when evaluating grass species for possible greenhouse gas mitigation strategies.


Author(s):  
Jentsje van der Meer ◽  
Gosse Jan Steendam ◽  
Cesare A. Mosca ◽  
Luca Bolatti Guzzo ◽  
Koichi Takata ◽  
...  

A dike or levee will protect a polder to build in a tropical country against coastal flooding. To ensure that the performance of the dike is in accordance with the safety standard, wave overtopping tests with a wave overtopping simulator have been performed on a mock-up dike. These wave overtopping tests will guide the selection of the grass species and topsoil for the grass cover of the landward side of the dike. The paper describes the design of a new dedicated wave overtopping simulator, the construction of the mock-up dike, the results of the eight tests on the mock-up dike and the critical velocities (strength indicator of the grass cover) established with the cumulative overload method.Recorded Presentation from the vICCE (YouTube Link): https://youtu.be/MCuKojNyNyQ


1972 ◽  
Vol 23 (1) ◽  
pp. 57-63 ◽  
Author(s):  
N.W.H. Cheetham ◽  
R.J. McIlroy ◽  
D.J. Winzor
Keyword(s):  

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