Memories of My Trap Line

2020 ◽  
pp. 49-55
Keyword(s):  
Trap Line

Fluorescent protein-based imaging and tissue-specific RNA-seq analysis of Arabidopsis hydathodes

2020 ◽  
Author(s):  
Hiroki Yagi ◽  
Atsushi J Nagano ◽  
Jaewook Kim ◽  
Kentaro Tamura ◽  
Nobuyoshi Mochizuki ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Small Cells ◽  
Rna Seq ◽  
Water Release ◽  
Tissue Specific ◽  
Enhancer Trap Line ◽  
Physiological Analysis ◽  
Green Fluorescent ◽  
Trap Line ◽  
Lower Expression

Abstract Hydathodes are typically found at leaf teeth in vascular plants and are involved in water release to the outside. Although morphological and physiological analysis of hydathodes has been performed in various plants, little is known about the genes involved in hydathode function. In this study, we performed fluorescent protein-based imaging and tissue-specific RNA-seq analysis in Arabidopsis hydathodes. We used the enhancer trap line E325, which has been reported to express green fluorescent protein (GFP) at its hydathodes. We found that E325-GFP was expressed in small cells found inside the hydathodes (named E cells) that were distributed between the water pores and xylem ends. No fluorescence of the phloem markers pSUC2:GFP and pSEOR1:SEOR1-YFP was observed in the hydathodes. These observations indicate that Arabidopsis hydathodes are composed of three major components: water pores, xylem ends, and E cells. In addition, we performed transcriptome analysis of the hydathode using the E325-GFP line. Microsamples were collected from GFP-positive or -negative regions of E325 leaf margins with a needle-based device (~130 µm in diameter). RNA-seq was performed with each single microsample using a high-throughput library preparation method called Lasy-Seq. We identified 72 differentially expressed genes. Among them, 68 genes showed significantly higher and four genes showed significantly lower expression in the hydathode. Our results provide new insights into the molecular basis for hydathode physiology and development.

Characterization of a T-DNA promoter trap line of Arabidopsis thaliana uncovers a cryptic bi-directional promoter

Gene ◽  
2013 ◽  
Vol 524 (1) ◽  
pp. 22-27 ◽  
Author(s):  
Pritu Pratibha ◽  
Sunil Kumar Singh ◽  
Isha Sharma ◽  
Ravi Kumar ◽  
Ramamurthy Srinivasan ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Promoter Trap ◽  
Trap Line

scholarly journals Zebrafish enhancer trap line showing maternal and neural expression of kctd15a

2012 ◽  
Vol 54 (2) ◽  
pp. 241-252 ◽  
Author(s):  
Naouel Gharbi ◽  
Xiao-Feng Zhao ◽  
Staale Ellingsen ◽  
Anders Fjose
Keyword(s):  
Enhancer Trap ◽  
Enhancer Trap Line ◽  
Trap Line

THE BIOLOGY AND PHEROMONE-BASED MONITORING OF THE DRIEDFRUIT MOTH, VITULA EDMANDSAE SERRATILINEELLA (LEPIDOPTERA: PYRALIDAE)

1984 ◽  
Vol 116 (7) ◽  
pp. 1007-1013 ◽  
Author(s):  
Cynthia D. Scott ◽  
M. L. Winston ◽  
K. N. Slessor ◽  
G. G. S. King ◽  
G. G. Grant
Keyword(s):  
British Columbia ◽  
Control Program ◽  
Western Canada ◽  
Monitoring And Control ◽  
Trapping Period ◽  
Achroia Grisella ◽  
Lepidoptera Pyralidae ◽  
And Control ◽  
Fraser Valley ◽  
Trap Line

AbstractIn western Canada, three wax-infesting moth species are serious pests of honey bee products, Vitula edmandsae serratilineella Ragonot, Achroia grisella (F.), and Plodia interpunctella (Hübner). The latter two species are found only in the Fraser Valley of British Columbia, while the driedfruit moth (sometimes called the bumble bee wax moth), V. edmandsae, is found throughout western Canada.Three female-produced pheromone components of V. edmandsae were identified as (Z,E)-9,12-tetradecadien-1-ol, (Z)-9-tetradecen-1-ol, and (Z)-11-hexadecen-1-ol, and field tested. Pheromone baits were used in traps outdoors and in bee equipment storage facilities in the Fraser and Okanagan valleys of British Columbia. The peak indoor trapping period occurred during May in the Okanagan Valley, while catches in indoor traps in the Fraser Valley were negligible throughout the entire trapping period. Catches in outdoor traps peaked during July in both regions. Traps baited with (Z,E)-9,12-tetradecadien-1-ol, alone or in binary combination with (Z)-11-hexadecen-1-ol, were significantly more attractive to male V. edmandsae than all other treatments tested. At one apiary, cumulative moth catch from a trap line 1.0 m from hives was significantly greater than that from a trap line 4.5 m away. The results suggest that a pheromone-based monitoring and control program for V. edmandsae is feasible.

scholarly journals A Protein-trap allele reveals roles for Drosophila ATF4 in photoreceptor degeneration, oogenesis and wing development

2021 ◽  
Author(s):  
Deepika Vasudevan ◽  
Hidetaka Katow ◽  
Huai-Wei Huang ◽  
Grace Tang ◽  
Hyung Don Ryoo
Keyword(s):  
Control Mechanisms ◽  
Loss Of Function ◽  
Pupal Development ◽  
Gfp Fusion Protein ◽  
Drosophila Model ◽  
Evolutionarily Conserved ◽  
Age Dependent ◽  
Cellular Stressors ◽  
Trap Line

Metazoans have evolved various quality control mechanisms to cope with cellular stress inflicted by external and physiological conditions. ATF4 is a major effector of the Integrated Stress Response (ISR), an evolutionarily conserved pathway that mediates adaptation to various cellular stressors. Loss of function of Drosophila ATF4, encoded by the gene cryptocephal (crc), results in lethality during pupal development. The roles of crc in Drosophila disease models and in adult tissue homeostasis thus remain poorly understood. Here, we report that a protein-trap MiMIC insertion in the crc locus generates a crc-GFP fusion protein that allows visualization of crc activity in vivo. This allele also acts as a hypomorphic mutant that uncovers previously unknown roles for crc. Specifically, the crc protein-trap line shows crc-GFP induction in a Drosophila model for Retinitis Pigmentosa (RP). This crc allele renders flies more vulnerable to amino acid deprivation and age-dependent retinal degeneration. These mutants also show defects in wing veins and oocyte maturation. Together, our data reveal previously unknown roles for crc in development, cellular homeostasis and photoreceptor survival.

scholarly journals An Arabidopsis Minute-like phenotype caused by a semi-dominant mutation in a RIBOSOMAL PROTEIN S5 gene

Development ◽  
2001 ◽  
Vol 128 (21) ◽  
pp. 4289-4299 ◽  
Author(s):  
Dolf Weijers ◽  
Marry Franke-van Dijk ◽  
Robert-Jan Vencken ◽  
Ab Quint ◽  
Paul Hooykaas ◽  
...  
Keyword(s):  
Cell Division ◽  
Ribosomal Protein ◽  
Protein Translation ◽  
General Importance ◽  
Promoter Trap ◽  
Dividing Cells ◽  
Dna Insertion ◽  
Early Embryonic Stage ◽  
Higher Eukaryotes ◽  
Trap Line

Mutations in ribosomal protein (RP) genes in Drosophila lead to strong developmental phenotypes, expressed in the semi-dominant Minute syndrome. In plants, however, mutations in RP genes have so far only been reported to result in recessive developmental phenotypes. We present the analysis of an Arabidopsis promoter-trap line, in which a T-DNA insertion in an RPS5 gene (AtRPS5A) causes semi-dominant developmental phenotypes. Most cell-division processes are delayed or disturbed in the heterozygous mutant, and development is completely arrested at an early embryonic stage in the homozygous mutant. By analogy with Drosophila rp mutants, we have named this mutant Arabidopsis Minute-like 1 (aml1). As with other Arabidopsis RPs, RPS5 is represented by a small gene family, but in contrast to other described plant RPs, this family comprises only two members. The AtRPS5A gene (mutated in aml1) is strongly expressed in dividing cells, whereas expression of the second RPS5 gene, AtRPS5B, is lower than that of AtRPS5A, and is correlated with cell differentiation rather than cell division. From expression analyses we conclude that AtRPS5A is the most abundantly expressed RPS5 gene in Arabidopsis. The Minute-like defects in the aml1 mutant provide the first evidence that ribosome insufficiency leads to similar consequences in both plants and insects, and emphasize the general importance of efficient protein translation for cell proliferation in higher eukaryotes.

The Trap Line as a Measure of Small Mammal Populations

1948 ◽  
Vol 12 (2) ◽  
pp. 153 ◽  
Author(s):  
Lucille F. Stickel
Keyword(s):  
Small Mammal ◽  
Trap Line

scholarly journals Molecular identification of a root apical cell-specific and stress-responsive enhancer from an Arabidopsis enhancer trap line

Plant Methods ◽  
2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Lei Zhang ◽  
Li-Na Qin ◽  
Zi-Rui Zeng ◽  
Chang-Zheng Wu ◽  
Yuan-Yong Gong ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Apical Cell ◽  
Enhancer Trap ◽  
Enhancer Trap Line ◽  
Trap Line
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