EFFECTS OF 1-alpha-OHD3 ON INTESTINAL RADIOCALCIUM ABSORPTION AND SERUM BONE GLA PROTEIN IN NORMAL SUBJECTS AND OSTEOPOROTIC WOMEN.

Vitamin D ◽  
1988 ◽  
pp. 458-459
Keyword(s):  
Normal Subjects ◽  
Bone Gla Protein

Measurement of serum bone gla-protein (BGP) in humans with an ovine BGP-based radioimmunoassay

1990 ◽  
Vol 36 (9) ◽  
pp. 1620-1624 ◽  
Author(s):  
P Pastoureau ◽  
P D Delmas
Keyword(s):  
Amino Acids ◽  
Primary Hyperparathyroidism ◽  
Renal Osteodystrophy ◽  
Clinical Investigation ◽  
Gel Filtration ◽  
Normal Subjects ◽  
Conformational Epitope ◽  
Cross Reactivity ◽  
Bone Gla Protein ◽  
Human Sera

Abstract Most RIAs of serum bone gla-protein (BGP; also called osteocalcin) used for clinical investigation are based on bovine BGP for standard, tracer, and immunogen because of the homology between bovine and human BGP. However, ovine BGP differs from human BGP by only five amino acids, being identical from residues 11 to 49, as compared with homology at residues 20-49 between bovine and human BGP. In screening various anti-ovine BGP polyclonal anti-sera we selected one (R310) that exhibits apparently complete cross-reactivity with human BGP, as assessed by dilutions of 13 human sera from normal subjects and from patients with bone disease. This RIA gave a 42% binding at a 10,000-fold final dilution, with intra- and interassay variations less than 7% and 11%, respectively. Gel-filtration chromatography of human serum showed a single immunoreactive peak. Synthetic fragments of human BGP 1-10, 7-19, 25-37, and 37-49 were not recognized by R310, suggesting that either a mid-molecule region or a conformational epitope was its target. Using this RIA, we determined that serum BGP increased with age in women (P less than 0.02), by a mean of 90% from ages 30 to 70 years. Serum BGP was also increased in patients with primary hyperparathyroidism, renal osteodystrophy, and Paget's disease. In contrast with the "normal" concentrations of BGP detected with an anti-bovine BGP antiserum (R102), serum BGP was increased in patients with postmenopausal osteoporosis as measured with the R310 ovine assay, suggesting a greater sensitivity for the latter assay.

Two-Site Assays of Bone Gla Protein (Osteocalcin) Demonstrate Immunochemical Heterogeneity of the Intact Molecule

1992 ◽  
Vol 38 (11) ◽  
pp. 2318-2321 ◽  
Author(s):  
L J Deftos ◽  
R L Wolfert ◽  
C S Hill ◽  
D W Burton
Keyword(s):  
Renal Failure ◽  
Monoclonal Antibodies ◽  
Clinical Studies ◽  
Normal Subjects ◽  
Human Bone ◽  
Bone Gla Protein ◽  
Serum Concentrations ◽  
Paget Disease ◽  
Linear Sequence ◽  
Intact Molecule

Abstract We developed a panel of monoclonal antibodies to human bone gla protein (BGP; osteocalcin) peptides that span the linear sequence of the molecule, specifically BGP 1-12 (N-terminal), BGP 15-30 (midregion), and BGP 38-49 (C-terminal). These antibodies were evaluated in various combinations of two-site formats in studies of serum BGP concentrations. For clinical studies, we selected from a panel of antibodies the two most sensitive antibody pairs for the intact molecule (N-C); we also used a polyclonal RIA based on BGP-C. For the two-site format, we used two N-terminal antibodies, 029 and 052, adsorbed to polystyrene beads, and radioiodinated a C-terminal antibody, 663. The standard for each of the assays was purified human BGP. The following BGP serum concentrations (microgram/L, mean +/- SE) were measured with the various assays: by the 029-663 assay, results for normal subjects were 7 +/- 3, for patients with renal failure 25 +/- 8, and for patients with Paget disease 12 +/-4; by the 052-663 assay, the respective results were 22 +/- 4, 44 +/- 12, and 31 +/- 7; by the polyclonal assay, the results were 3 +/- 0.2, 13 +/- 2, and 5 +/- 1. The two intact (N-C) assays were significantly (P < 0.01) correlated (r = 0.94), but their serum values differed by more than twofold in terms of the same BGP standard. The polyclonal assay significantly correlated with each of the intact assays (r = 0.83, 0.77), but it, too, gave different serum values for BGP. These studies demonstrate the immunochemical heterogeneity of circulating BGP, heterogeneity that is manifest even in immunoassays specific for the same region of the molecule.

A radioimmunoassay for bone Gla protein (BGP) in human plasma

1987 ◽  
Vol 114 (3) ◽  
pp. 410-416 ◽  
Author(s):  
Julia S. Johansen ◽  
J. E. Mølholm Hansen ◽  
Claus Christiansen
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Human Plasma ◽  
Biochemical Marker ◽  
Normal Subjects ◽  
Bone Gla Protein ◽  
Low Concentrations ◽  
Metabolic Bone ◽  
The Mean ◽  
Bone Physiology

Abstract. To study the value of bone Gla protein (BGP) as a biochemical marker of normal bone physiology and metabolic bone disorders, we have developed a radioimmunoassay (RIA) for the detection of BGP in human plasma. Antibodies were generated in rabbits immunized with purified calf BGP conjugated to thyroglobulin. Human plasma BGP reacted identically with the calf BGP standard, thus demonstrating the suitability of the assay to measure plasma BGP levels in man. The RIA is sensitive, accurate, and technically simple. Plasma BGP levels were determined in normal subjects (N = 35) and in patients with hypothyroidism (N = 10), hyperthyroidism (N = 22) and chronic renal failure (N = 35). The mean (± 1 sem) concentration of plasma BGP in normal subjects was 1.27 ± 0.07 nmol/l. Plasma BGP was significantly increased in patients with hyperthyroidism, 4.04 ± 0.78 nmol/l (P < 0.001) and chronic renal failure, 10.17 ± 2.47 nmol/l (P < 0.001). Low concentrations were found in patients with hypothyroidism, 0.74 ± 0.11 nmol/l (P <0.01). Our studies indicate that plasma BGP provides a useful technique in the diagnosis of patients with bone disease.

Auditory Selective Attention in Cerebral-Palsied Individuals

1985 ◽  
Vol 16 (4) ◽  
pp. 260-266 ◽  
Author(s):  
Lee Ann Laraway
Keyword(s):  
Selective Attention ◽  
White Noise ◽  
Normal Subjects ◽  
Auditory Selective Attention ◽  
Normal Individuals ◽  
Significant Difference

The purpose of this study was to determine whether there is a statistically significant difference between the auditory selective attention abilities of normal and cerebral-palsied individuals. Twenty-three cerebral-palsied and 23 normal subjects between the ages of 5 and 21 were asked to repeat a series of 30 items consisting of from 2 to 4 digits in the presence of intermittent white noise. Results of the study indicate that cerebral-palsied individuals perform significantly poorer than normal individuals when the stimulus is accompanied by noise. Noise was not a significant factor in the performance of the normal subjects regardless of age.

The Effect of Stapedectomy on the Loudness of One’s Own Voice

1965 ◽  
Vol 8 (3) ◽  
pp. 223-234 ◽  
Author(s):  
William Melnick
Keyword(s):  
Middle Ear ◽  
Normal Subjects ◽  
Sound Energy ◽  
Loudness Function ◽  
Before And After ◽  
Increased Sensitivity

Five subjects with normal middle ear mechanisms, and otosclerotic patients, before and after stapedectomy, matched the loudness of their voices to the loudness of a 125-cps-sawtooth noise. The results showed loudness matching functions with gradual slopes, less than 1.00, for the normal subjects and the patients prior to stapedectomy. Post-surgically, the loudness function for the patients increased in steepness to considerably more than 1.00. These results are explained, most logically, in terms of increased sensitivity of the altered middle ear to sound energy generated by the listener’s own voice.

Delayed Auditory Feedback with Different Delay Times at Each Ear

1964 ◽  
Vol 7 (4) ◽  
pp. 369-371
Author(s):  
Samuel Fillenbaum
Keyword(s):  
Delay Interval ◽  
Auditory Feedback ◽  
Normal Subjects ◽  
Delayed Auditory Feedback ◽  
Delay Times

Binaurally asynchronous delayed auditory feedback (DAF) was compared with synchronous DAF in 80 normal subjects. Asynchronous DAF (0.10 sec difference) did not yield results different from those obtained under synchronous DAF with a 0.20 sec delay interval, an interval characteristically resulting in maximum disruptions in speech.

Object Transformations in the Play of Language-Impaired Children

1988 ◽  
Vol 53 (4) ◽  
pp. 459-466 ◽  
Author(s):  
Brenda Y. Terrell ◽  
Richard G. Schwartz
Keyword(s):  
Normal Subjects ◽  
Play Behavior ◽  
Chronological Age ◽  
Standard Group ◽  
Language Impaired ◽  
Normal Language ◽  
Mean Length Of Utterance ◽  
Impaired Children

The play behavior of 10 language-impaired children was observed. Their performances in play were compared to those of 10 normal-language children matched for chronological age as well as to those of 10 normal-language children matched for mean length of utterance. The children were observed as they played spontaneously with a standard group of toys and as they played with objects that required object transformations for successful play. The chronological age-matched normal subjects showed a trend toward performance of more object transformations in play than either the language-impaired or younger normal-language children. Additionally, although object transformations were observed in both segments, all children performed more object transformations with objects than with toys.

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