scholarly journals Somatotropic gene response to recombinant growth hormone treatment in buffalo leucocytes

2011 ◽  
Vol 392 (12) ◽  
pp. 1145-1154 ◽  
Author(s):  
Lorenzo Castigliego ◽  
Xiao-Ning Li ◽  
Andrea Armani ◽  
Maria Razzano ◽  
Marco Mazzi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Hormone ◽  
Dairy Products ◽  
Hormone Treatment ◽  
Recombinant Growth Hormone ◽  
Release Formulation ◽  
Somatotropic Axis ◽  
Sustained Release Formulation ◽  
Lack Of Information ◽  
Gene Response

Abstract The use of recombinant bovine growth hormone (rbGH) to increase milk yield in cows is banned in some countries. In others, where it is authorised, it has triggered harsh debates on labelling of dairy products. If many studies have been performed on bovines, there is a lack of information on buffaloes, which are sometimes treated with rbGH and re­present an important economical resource for dairy products in some countries. Analytical methods with legal value for surveillance of rbGH treatments do not yet exist. Research on gene expression biomarkers is one of the most promising approaches to this purpose. For this reason, we treated five buffaloes for 10 weeks with a sustained-release formulation of rbGH and analysed the response of 20 somatotropic axis genes in leucocytes by real-time polymerase chain reaction. Overall changes in gene expression levels were of low magnitude and sometimes affected by the ‘time’ factor. Only the IGFBP-1 gene showed a significant under-expression (about two-fold; p <0.001) in treated animals. Taken together, these results give evidence that expression analysis of the somatotropic axis genes in leuco­cytes is little helpful for discrimination of rbGH-treated buffaloes, but do not exclude that another array of genes could provide useful patterns of variation.

Long-Term Mortality after Recombinant Growth Hormone Treatment for Isolated Childhood Short Stature: Report of the French SAGhE Study

2011 ◽  
pp. LB-5-LB-5 ◽  
Author(s):  
Jean-Claude Carel ◽  
Emmanuel Ecosse ◽  
Fabienne Landier ◽  
Djamila Meguellati-Hakkas ◽  
Florentia Kaguelidou ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Short Stature ◽  
Growth Hormone Treatment ◽  
Hormone Treatment ◽  
Recombinant Growth Hormone ◽  
Long Term Mortality

Effect of residual feed intake phenotype–nutritional treatment interaction on the growth performance, plasma metabolic variables and somatotropic axis gene expression of growing ewes

2016 ◽  
Vol 56 (10) ◽  
pp. 1593 ◽  
Author(s):  
H. T. Nie ◽  
Z. Y. Wang ◽  
S. Lan ◽  
H. Zhang ◽  
Y. J. Wan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Hormone ◽  
Growth Performance ◽  
Feed Intake ◽  
Mrna Abundance ◽  
Feed Restriction ◽  
Somatotropic Axis ◽  
Nutritional Treatment ◽  
Treatment Interaction ◽  
Ad Libitum

This study aimed to evaluate the effect of residual feed intake (RFI) phenotype and nutritional treatment interaction on the growth performance, plasma variables and gene expression levels within the somatotropic axis. Growing ewes [n = 52, initial bodyweight (BW) = 17.5 ± 0.5 kg, 2 months of age] were offered ad libitum access to diets for 63 days and ranked based on RFI phenotype. Thirty ewes with the highest and lowest RFI values were selected and randomly assigned to three nutritional treatments based on dry matter intake (DMI), which are ad libitum (AL), low restriction (LR) and high restriction (HR) groups, respectively. Each nutritional treatment group included ewes with high (n = 5) and low RFI (n = 5) values. During nutritional treatment (from Day 64 to Day 138), plasma samples were obtained to measure metabolite and hormone concentrations. Tissues of the hypothalamus, pituitary, liver, and Longissimus dorsi muscle (LM) were harvested at the end of the experiment (Day 138) to measure the gene expression level within the somatotropic axis. Muscle growth hormone receptor mRNA abundance of low RFI ewes tended to be greater (P = 0.09) under AL feeding, but this difference was abolished by underfeeding (P > 0.10). Low RFI ewes under HR treatment showed slightly greater growth performance, which was accompanied with lower pituitary somatostain receptor 2 mRNA abundance (P < 0.05), plasma non-esterified fatty acid concentration (P < 0.05), and greater concentration of triglyceride (P < 0.05), compared with ewes classified as high RFI group. Our results suggested that ewes categorised as low RFI showed higher resistance to the condition of high feed restriction, which might be attributed to less intensity of fat mobilisation under negative energy balance. The mechanism underlying resistance to such feed restriction was presumably through action of somatostain receptor 2 and was potentially mediated by inhibitory effects of somatostatin on growth hormone release but not basal growth hormone secretion.

Differences in the somatotropic axis, in blood cortisol, insulin and thyroid hormone concentrations between two pig genotypes with markedly divergent growth rates and the effects of growth hormone treatment

2002 ◽  
Vol 74 (3) ◽  
pp. 423-430 ◽  
Author(s):  
F. Elsaesser ◽  
M. W. Pfaffl ◽  
H. H. D. Meyer ◽  
B. Serpek ◽  
H. Sauerwein
Keyword(s):  
Growth Hormone ◽  
Live Weight ◽  
Maximum Level ◽  
Pulse Amplitude ◽  
Hormone Treatment ◽  
Pulse Frequency ◽  
Growth Potential ◽  
Control Mechanisms ◽  
Somatotropic Axis ◽  
Molecular Control

AbstractThe intention of the current study was to gain more insight into the endocrine and molecular control mechanisms of growth in the pig. For this purpose various growth related parameters were determined in 4-month-old barrows of two extreme pig genotyes, the small, obese Göttingen Miniature (GM) and the large and lean German Landrace (DL). Mean growth hormone (GH) concentration, GH pulse frequency and GH pulse amplitude did not differ between breeds. Likewise, plasma IGF-1, thyroxine, tri-iodothyronine (T3) concentrations were similar in both breeds. However the plasma GH response (maximum level and area under curve) to a single i.v. injection of GHRH in DL was higher than in GM (P < 0·05). Furthermore, basal plasma insulin and in particular plasma cortisol concentrations were higher in GM compared with DL pigs (P 0·05 and 0·01 respectively). Analysis of cortisol during 4-h frequent blood sampling indicated higher cortisol amplitudes in GM compared with DL (P ≤ 0·01). Specific bGH-binding to hepatic membrane preparations was not different between breeds and IGF-1 m RNA concentrations determined by reverse transcription-polymerase chain reaction in liver, m. semimenbranosus and m. longissimus dorsi were similar in both breeds. I.m. treatment with recombinant porcine somatotropin (rpST; 70 µg/kg live weight) over an 8-day period in contemporary barrows increased without any breed difference, plasma IGF-1, T3 and insulin concentrations and hepatic specific bGH-binding, but did not affect thyroxine or cortisol concentrations in plasma. IGF-1 gene expression was also elevated in liver and muscle tissues in rpST-treated animals without obvious breed effects. The observations underline the complexity of the hormonal and molecular control of growth and support the notion that differences in growth potential are the consequence of differences at various levels of the somatotropic axis and apparently relate to differences in other control systems of energy metabolism such as the pituitary adrenal axis or the endocrine pancreas as well.

Glucose Tolerance and Insulin Secretion in Children before and during Recombinant Growth Hormone Treatment

1998 ◽  
Vol 50 (1) ◽  
pp. 32-37 ◽  
Author(s):  
Guido Filler ◽  
Peter Amendt ◽  
Klaus-Dieter Kohnert ◽  
Siegmar Devaux ◽  
Jochen H.H. Ehrich
Keyword(s):  
Growth Hormone ◽  
Insulin Secretion ◽  
Glucose Tolerance ◽  
Growth Hormone Treatment ◽  
Hormone Treatment ◽  
Recombinant Growth Hormone
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