scholarly journals Different pattern of haemagglutinin immunoreactivity of equine influenza virus strains isolated in Poland

2015 ◽  
Vol 59 (4) ◽  
pp. 463-466
Author(s):  
Małgorzata Kwaśnik ◽  
Ilona Marcelina Góra ◽  
Wojciech Rożek

Abstract The immunoreactivity of haemagglutinin (HA) polypeptides of equine influenza virus was compared among the strains isolated in Poland, using H3 monoclonal antibody. A stronger signal in immunoblot reaction was observed for A/equi/Pulawy/2008 HA polypeptides compared to A/equi/Pulawy/2006, despite the fact that both strains are phylogenetically closely related and belong to Florida clade 2 of American lineage. The strongest signal, observed in the case of A/equi/Pulawy/2008, seemed to be connected with the presence of G135, I213, E379, and/or V530 instead of R135, M213, G379, and I530 present in A/equi/Pulawy/2006 HA sequence. This implies that point mutations within amino acid sequences of HA polypeptides of equine influenza virus may change their immunoreactivity even when they are not located within five basic antigenic sites.

Virology ◽  
2017 ◽  
Vol 505 ◽  
pp. 210-217 ◽  
Author(s):  
Stéphanie Fougerolle ◽  
Loïc Legrand ◽  
Fanny Lecouturier ◽  
Corinne Sailleau ◽  
Romain Paillot ◽  
...  

2018 ◽  
Vol 6 (12) ◽  
Author(s):  
Manabu Nemoto ◽  
Takashi Yamanaka ◽  
Hiroshi Bannai ◽  
Koji Tsujimura ◽  
Hiroshi Kokado

ABSTRACT We sequenced the eight segments of influenza A virus strains A/equine/Ibaraki/1/2007 and A/equine/Yokohama/aq13/2010, which are strains of the Florida sublineage clades 1 and 2 of the H3N8 subtype equine influenza virus. These strains have been used as vaccine strains in Japan since 2016 in accordance with World Organization for Animal Health (OIE) recommendations.


1999 ◽  
Vol 37 (9) ◽  
pp. 3005-3009 ◽  
Author(s):  
L. Oxburgh ◽  
B. Klingeborn

Direct amplification and sequencing of the hemagglutinin (HA) genes of equine influenza virus subtype H3N8 was undertaken in order to characterize strains of this virus circulating in Sweden. The majority of viruses from outbreaks during 1997 analyzed belonged to the American lineage of H3 equine influenza, and one strain was shown to belong to the European lineage. Furthermore, it was shown that recent American-lineage strains are mutated at amino acid position 190 of the HA during serial passage in embryonated hens’ eggs. Host cell adaptation of these viruses thus takes place at antigenic region B of the HA.


2018 ◽  
Vol 6 (26) ◽  
Author(s):  
Yerbol Burashev ◽  
Vitaliy Strochkov ◽  
Kulyaisan Sultankulova ◽  
Mukhit Orynbayev ◽  
Abylay Sansyzbay ◽  
...  

Here, we report the complete genome sequencing of strains A/equine/Kostanay/9/2012(H3N8) and A/equine/LKZ/9/2012(H3N8) of the equine influenza virus belonging to Florida sublineage, clade 2. The strains were isolated in 2012 in the northern and southern regions of Kazakhstan, respectively.


2009 ◽  
Vol 138 (1-2) ◽  
pp. 41-52 ◽  
Author(s):  
Neil A. Bryant ◽  
Adam S. Rash ◽  
Colin A. Russell ◽  
Julie Ross ◽  
Annie Cooke ◽  
...  

2016 ◽  
Vol 10 (6) ◽  
pp. 536-539 ◽  
Author(s):  
Takashi Yamanaka ◽  
Manabu Nemoto ◽  
Hiroshi Bannai ◽  
Koji Tsujimura ◽  
Takashi Kondo ◽  
...  

2013 ◽  
Vol 16 (4) ◽  
pp. 663-669
Author(s):  
W. Rozek ◽  
M. Kwasnik ◽  
J.F. Zmudzinski

AbstractChanges in the level of cellular proteins in cells inoculated with equine influenza virus H7N7 and H3N8 were studied with microarray technique. H3N8 induced pro-apoptotic proteins while H7N7 induced both pro- as well as anti-apoptotic factors. The higher level of some cytoskeleton components and proteins involved in the protein quality control was recorded. Relatively high number of proteins involved in the regulation of transcription was down-regulated. The pattern of changes observed for H7N7 and H3N8 may reflect differences in the biological properties of both serotypes.


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