Follicular Fluid Renin Concentration in Patients with Polycystic Ovaries Treated with Gonadotrophins in an in vitro Fertilisation Programme

2003 ◽  
Vol 41 (5) ◽  
Author(s):  
Eda Vrtačnik-Bokal ◽  
Helena Meden-Vrtovec ◽  
Joško Osredkar ◽  
Ivan Verdenik
Keyword(s):  
Follicular Fluid ◽  
In Vitro Fertilisation ◽  
Polycystic Ovaries

scholarly journals Significance of disorders of proteomic profile of follicular fluid for predicting the effectiveness of in vitro fertilisation in women with infertility

2018 ◽  
Vol 99 (3) ◽  
pp. 496-503
Author(s):  
O S Zolotykh ◽  
S V Lomteva ◽  
K Yu Sagamonova
Keyword(s):  
Assisted Reproductive Technology ◽  
Follicular Fluid ◽  
Molecular Mechanisms ◽  
Reproductive Technologies ◽  
Reproductive Technology ◽  
In Vitro Fertilisation ◽  
Proteomic Profile ◽  
Technology Programs ◽  
Group 2

Aim. To study the proteomic profile of follicular fluid in patients with infertility in assisted reproductive technology programs. Methods. The study included women with infertility included in assisted reproductive technology programs: 15 women who had in vitro fertilisation which resulted in pregnancy (group 1) and 16 women with a negative result of this program (group 2). Fractionation of the follicular fluid samples was performed using the sets of special magnetic beads. Proteomic profiling was performed by tandem MALDI-mass-spectrometry. The anti-Müllerian hormone level was measured by ELISA. Results. The study revealed differences in the detectability of follicular fluid proteins with different regulatory properties in patients of groups 1 and 2. With the negative outcome of in vitro fertilisation, expression of a number of proteins involved in the processes of folliculogenesis, ovulation, selection of the dominant follicle, as well as proteins necessary for the development of the zygote and blastula was reduced in females' follicular fluid. Increased expression in women from group 2 was registered for proteins enhancing proteolytic reactions, cell apoptosis, including oocytes, which disrupt the positive action of activin and damage structural and functional state of mitochondria. A definite relationship was found between the level of anti-Müllerian hormone and rate of detection of a number of proteins, in particular protocadherin-2α, cystatin C, betaglycan, prostatic acid phosphatase, and dermicidin. Conclusion. The revealed changes in proteomic profile of the follicular fluid obviously play an important role in the molecular mechanisms that determine the effectiveness of assisted reproductive technologies; the identified differentially expressed proteins can serve as objective markers for predicting the outcomes of in vitro fertilisation.

scholarly journals Function of Follicular Cytokines: Roles Played during Maturation, Development and Implantation of Embryo

Medicina ◽  
2021 ◽  
Vol 57 (11) ◽  
pp. 1251
Author(s):  
Rafał Adamczak ◽  
Natalia Ukleja-Sokołowska ◽  
Kinga Lis ◽  
Mariusz Dubiel
Keyword(s):  
Follicular Fluid ◽  
Embryo Implantation ◽  
Ovarian Follicles ◽  
In Vitro Fertilisation ◽  
Test Results ◽  
Patient Preparation ◽  
The Many ◽  
Reproductive Pathology

A balance within the immune system is necessary for the proper development of ovarian follicles. Numerous cytokines were detected in follicular fluid, the role of which in reproductive physiology seems crucial. They influence the development and maturation of the follicle, ovulation, and corpus luteum formation, as well as embryo implantation and maintenance of pregnancy. The analysis of follicular fluid requires its collection by puncturing of the ovary, which is usually executed in connection with various gynaecological procedures. When interpreting such test results, clinical indications for a given procedure and the method of patient preparation should be taken into account. This review revealed the results of currently available studies on the concentration of pro-inflammatory cytokines in follicular fluid in various forms of infertility. Additionally, it presented cytokines, whose concentration has a significant impact on the size of ovarian follicles, their number, the effectiveness of in vitro fertilisation, development of the embryo, and chances of correct implantation. Despite the many recent publications, the knowledge of follicular fluid immunology in the context of reproductive pathology is superficial and further research is required to extensively understand the roles of individual cytokines in reproductive pathology. In the future, this knowledge may enable patients’ individual qualifications to individual methods of infertility treatment, as well as the possible adjustment of the treatment regimen to the patient’s immune profile.

Follicular fluid supplementation during in vitro maturation promotes sperm penetration in bovine oocytes by enhancing cumulus expansion and increasing mitochondrial activity in oocytes

2012 ◽  
Vol 24 (5) ◽  
pp. 743 ◽  
Author(s):  
Tamás Somfai ◽  
Yasushi Inaba ◽  
Shinya Watanabe ◽  
Masaya Geshi ◽  
Takashi Nagai
Keyword(s):  
Embryo Development ◽  
Follicular Fluid ◽  
Calf Serum ◽  
In Vitro Fertilisation ◽  
Mitochondrial Activity ◽  
Atp Content ◽  
Promoting Effect ◽  
Cumulus Expansion ◽  
Sperm Penetration

The aim of this study was to examine the effects of bovine follicular fluid (bFF) on mitochondrial activity in in vitro-matured (IVM) oocytes and to assess its importance for fertilisation and embryo development. Bovine follicular oocytes were subjected to IVM in medium supplemented either with polyvinylpyrrolidone, bovine serum albumin, calf serum or bFF. Nuclear maturation, cumulus expansion, mitochondrial distribution and ATP content in oocytes were compared between groups along with subsequent in vitro fertilisation (IVF) and embryo development. Compared with other supplements, bFF generated significantly enhanced re-distribution of active mitochondria in oocytes and this effect was associated with elevated intracellular ATP content. Furthermore, bFF significantly improved cumulus expansion, which was associated with improved fertilisation rates when cumulus-enclosed oocytes were subjected to IVF; however, its promoting effect was neutralised when denuded oocytes were inseminated. Elevating ATP content in oocytes by bFF did not affect maturation or embryo development but promoted fertilisation when mitochondrial electron transport was blocked in oocytes before IVF by Rotenone. In conclusion, supplementation of IVM medium with bFF promotes sperm penetration both by the improvement of cumulus expansion and by enhancing ATP levels in oocytes, which maintains their ability to be fertilised after mitochondrial stress.

Increased anti-Müllerian hormone and decreased FSH levels in follicular fluid obtained in women with polycystic ovaries at the time of follicle puncture for in vitro fertilization

2010 ◽  
Vol 94 (1) ◽  
pp. 198-204 ◽  
Author(s):  
Virginie Desforges-Bullet ◽  
Cécile Gallo ◽  
Catherine Lefebvre ◽  
Pascal Pigny ◽  
Didier Dewailly ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Polycystic Ovaries ◽  
Vitro Fertilization

Chlamydial IgG and IgA in serum and follicular fluid among patients undergoing in vitro fertilisation

1990 ◽  
Vol 37 (2) ◽  
pp. 163-173 ◽  
Author(s):  
E. Lunenfeld ◽  
B. Sarov ◽  
I. Sarov ◽  
G. Potashnik ◽  
S. Albotiano ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
In Vitro Fertilisation

Vaspin, a novel adipokine in woman granulosa cells physiology and PCOS pathogenesis?

2021 ◽  
Author(s):  
Alice Bongrani ◽  
Namya Mellouk ◽  
Christelle Ramé ◽  
Marion Cornuau ◽  
Fabrice Guerif ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
Polycystic Ovary ◽  
Normal Weight ◽  
Human Reproduction ◽  
In Vitro Fertilisation ◽  
Obese Women ◽  
Human Ovary

Vaspin is a novel adipokine mainly expressed in visceral adipose tissue and closely related to obesity and insulin-resistance. Currently, data about its ovarian expression are limited to animal models and its role in human reproduction is largely unexplored. Our study’s aims were thento characterise vaspin expression in the human ovary and to study in vitro its effects on granulosa cells physiology. Secondly, we assessed vaspin and its receptor GRP78 variations in granulosa cells and follicular fluid of a cohort of 112 infertile women undergoing an in vitro fertilisation procedure and allocated to 3 groups, each including normal-weight and obese subjects: 34 PCOS patients, 33 women with isolated polycystic ovary morphology (ECHO group) and 45 controls. Vaspin and GRP78 expression in the ovary was assessed by immunohistochemistry, RT-qPCR and Western blot. Granulosa cells and follicular fluid were analysed by RT-qPCR and ELISA, respectively. In vitro, granulosa cells metabolism was studied after stimulation with recombinant human vaspin, with and without a small interfering RNA directed against GRP78. Vaspin was highly expressed in the human ovary and concentration-dependently enhanced granulosa cells steroidogenesis, proliferation and viability through GRP78 (p<0.0001). Vaspin levels in both granulosa cells and follicular fluid were significantly higher in obese women (p<0.0001) and in the normal-weight ECHO group (p<0.001), which also had the highest expression rates of GRP78 (p<0.05).Although further investigation is needed, vaspin appears as a novel modulator of human granulosa cells physiology and possibly plays a role in PCOS pathogenesis, notably protecting from insulin-resistance induced complications.

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