scholarly journals Significance of disorders of proteomic profile of follicular fluid for predicting the effectiveness of in vitro fertilisation in women with infertility

2018 ◽  
Vol 99 (3) ◽  
pp. 496-503
Author(s):  
O S Zolotykh ◽  
S V Lomteva ◽  
K Yu Sagamonova
Keyword(s):  
Assisted Reproductive Technology ◽  
Follicular Fluid ◽  
Molecular Mechanisms ◽  
Reproductive Technologies ◽  
Reproductive Technology ◽  
In Vitro Fertilisation ◽  
Proteomic Profile ◽  
Technology Programs ◽  
Group 2

Aim. To study the proteomic profile of follicular fluid in patients with infertility in assisted reproductive technology programs. Methods. The study included women with infertility included in assisted reproductive technology programs: 15 women who had in vitro fertilisation which resulted in pregnancy (group 1) and 16 women with a negative result of this program (group 2). Fractionation of the follicular fluid samples was performed using the sets of special magnetic beads. Proteomic profiling was performed by tandem MALDI-mass-spectrometry. The anti-Müllerian hormone level was measured by ELISA. Results. The study revealed differences in the detectability of follicular fluid proteins with different regulatory properties in patients of groups 1 and 2. With the negative outcome of in vitro fertilisation, expression of a number of proteins involved in the processes of folliculogenesis, ovulation, selection of the dominant follicle, as well as proteins necessary for the development of the zygote and blastula was reduced in females' follicular fluid. Increased expression in women from group 2 was registered for proteins enhancing proteolytic reactions, cell apoptosis, including oocytes, which disrupt the positive action of activin and damage structural and functional state of mitochondria. A definite relationship was found between the level of anti-Müllerian hormone and rate of detection of a number of proteins, in particular protocadherin-2α, cystatin C, betaglycan, prostatic acid phosphatase, and dermicidin. Conclusion. The revealed changes in proteomic profile of the follicular fluid obviously play an important role in the molecular mechanisms that determine the effectiveness of assisted reproductive technologies; the identified differentially expressed proteins can serve as objective markers for predicting the outcomes of in vitro fertilisation.

Androstenedione as a potential predictor of ovarian response in assisted reproductive technology programs

GYNECOLOGY ◽  
2020 ◽  
Vol 22 (1) ◽  
pp. 38-44
Author(s):  
Anna G. Burduli ◽  
Natalia A. Kitsilovskaya ◽  
Yuliya V. Sukhova ◽  
Irina A. Vedikhina ◽  
Tatiana Yu. Ivanets ◽  
...  
Keyword(s):  
Blood Serum ◽  
Assisted Reproductive Technology ◽  
Follicular Fluid ◽  
High Performance ◽  
Ovarian Response ◽  
Reproductive Technology ◽  
Transfer Program ◽  
Vitro Fertilization ◽  
Technology Programs

Aim. To assess the possibility of using androstenedione levels in blood serum and follicular fluid to predict ovarian response in assisted reproductive technology programs and to conduct a comparative analysis of the results obtained by 2 methods chemiluminescent immunoassay (CLIA) and high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS). Materials and methods. A prospective study included 55 couples who received in vitro fertilization/intracytoplasmic sperm injection and embryo transfer program therapy. The patients were divided into 3 groups depending on the ovarian response to stimulation: 1st (13 oocytes, n=4), 2nd (49 oocytes, n=27), 3rd (over 10 oocytes, n=24). Androstenedione levels were measured in blood serum obtained on the day of transvaginal ovarian puncture and in follicular fluid samples with CLIA and HPLC-MS/ MS methods at the laboratories of Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology. Results. On the day of transvaginal ovarian puncture, the serum androstenedione levels, which were measured by HPLC-MS/MS, were increasing with an increase of the number of oocytes obtained. The CLIA method revealed a difference in the androstenedione levels between the groups with the number of oocytes obtained of less than 3 and more than 10. Moreover, the androstenedione levels measured by CLIA were significantly different between the patient groups (p0.05). Comparison of serum androstenedione levels measured by CLIA and HPLC-MS/MS, showed high correlations between the values [=0.73 (p0.001)], which makes it possible to use both methods equally, given the existing equipment of the clinical base. Conclusion. Prediction of ovarian response to stimulation is an important step in assisted reproductive technology programs. Measuring androstenedione concentration in blood serum on the day of transvaginal ovarian puncture with highly specific methods (CLIA and HPLC-MS/MS) can be used to predict the degree of ovarian response along with the traditional assessment of the ovarian reserve based on determining anti-Mullerian hormone levels in the early follicular phase of the menstrual cycle.

Follicular fluid and assisted reproductive technology programs outcomes (literature review)

GYNECOLOGY ◽  
2020 ◽  
Vol 21 (6) ◽  
pp. 36-40
Author(s):  
Anna G. Burduli ◽  
Natalia A. Kitsilovskaya ◽  
Yuliya V. Sukhova ◽  
Irina A. Vedikhina ◽  
Tatiana Y. Ivanets ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Assisted Reproductive Technology ◽  
Follicular Fluid ◽  
Economic Cost ◽  
Reproductive Technology ◽  
Art Programs ◽  
Vitro Fertilization ◽  
Non Invasive ◽  
Established Fact

The review presents data on metabolites in the follicular fluid (FF) from the perspective of reproductive medicine and their use in order to predict outcomes of assisted reproductive technology (ART) programs. It considers various components of this biological medium (hormones, lipids, melatonin, etc.) with an assessment of their predictive value in prognosis of the effectiveness of in vitro fertilization (IVF) programs. The data on experimental directions in this field and the prospects for their use in clinical practice are presented. The article emphasizes that the growing clinical need and the unsolved problem of increasing the effectiveness of ART programs determine the need for further studies of the FF composition. Materials and methods. The review includes data related to this topic from foreign and Russian articles found in PubMed which were published in recent years. Results. Given the established fact of a direct effect of FF composition on growth and maturation of oocytes, and further, on the fertilization process, various FF metabolites are actively investigated as non-invasive markers of quality of oocytes/embryos. The article provides data on the experimental directions in this field and the prospects for their use in clinical practice. However, clinical studies of a relation between various FF metabolites levels and outcomes of IVF programs are contradictory. Conclusion. Owing large economic cost for treatment of infertility with IVF, there is need for expansion and intensification of studies to identify and use reliable predictors in prognosis of ART programs outcomes.

Specific features of molecular mechanisms of vaginal secretion in women with decline in sexual function in assisted reproductive technology programs

2021 ◽  
Vol 7_2021 ◽  
pp. 165-173
Author(s):  
Stenyaeva N.N. Stenyaeva N ◽  
Krasnyi A.M. Krasnyi ◽  
Khritinin D.F. Khritinin ◽  
Burduli A.G. Burduli A ◽  
Sadekova A.A. Sadekova A ◽  
...  
Keyword(s):  
Sexual Function ◽  
Assisted Reproductive Technology ◽  
Molecular Mechanisms ◽  
Reproductive Technology ◽  
Vaginal Secretion ◽  
Technology Programs

Trends in safe infertility treatment with assisted reproductive technologies

2020 ◽  
Vol 69 (4) ◽  
pp. 83-88
Author(s):  
Svetlana S. Paskar ◽  
Alla S. Kalugina ◽  
Anna G. Tkachuk
Keyword(s):  
Clinical Practice ◽  
Assisted Reproductive Technology ◽  
Assisted Reproductive Technologies ◽  
Perinatal Outcomes ◽  
Reproductive Technologies ◽  
Infertility Treatment ◽  
Reproductive Technology ◽  
Correct Prediction ◽  
Vitro Fertilization

The expansion of indications for assisted reproductive technology has led to significant implications for assisted reproductive technology (ART) programs worldwide. More than 7 million children in the world were born using ART. Modern clinical practice in the field of reproductive sciences is aimed not only at increasing the effectiveness, but also at the safety of treatment. ART, like any other type of therapy, may be combined with negative side effects. Both the correct prediction of the risks associated with treatment and a personalized approach ensure the absolute safety of infertility treatment using in vitro fertilization. In this regard, over the past decade, a number of new research approaches have been noted that use ART methods integrated into clinical practice: cycle segmentation with subsequent embryo transfer and the elective transfer of one embryo. New approaches provide a control in relation to ovarian stimulation and a reduction in the number of transferred embryos, which helps to minimize primarily adverse perinatal outcomes. Predicting the risks and outcomes of treatment using mathematical modeling is the application of good clinical practice.

scholarly journals Assisted Reproductive Technology and the Reproductive Behavior of Urban Youth (Experience of a Pilot Study)

REGIONOLOGY ◽  
2019 ◽  
Vol 27 (1) ◽  
pp. 138-155
Author(s):  
Tatiana M. Dadaeva ◽  
Victoria V. Baranova
Keyword(s):  
In Vitro Fertilization ◽  
Young People ◽  
Reproductive Health ◽  
Assisted Reproductive Technology ◽  
Urban Youth ◽  
Reproductive Technology ◽  
Vitro Fertilization ◽  
Technology Programs ◽  
The City

Introduction. The use of assisted reproductive technology in a situation of a demographic crisis can contribute to an increase in the birth rate among childless parents. The paper is of relevance as there is a need for a sociological analysis of the youth’s attitudes towards the use of assisted reproductive technology. The purpose of the article is to reveal significant factors affecting the youth’s attitudes towards assisted reproductive technology and its application, based on the results of a case study conducted among young people residing in the city of Saransk. Materials and Methods. The study used the methods of analysis, comparison and correlation. The data of a pilot survey conducted among young people residing in the city of Saransk and statistics were used as the empirical base of the study. The data were collected on the basis of the methodology developed by the authors according to quota sampling (by sex, age and place of residence). Results. The study has revealed the correlation between the respondents’ awareness of assisted reproductive technology and their level of education, age and marital status. It has been established that those respondents who took advantage of assisted reproductive technology, more often used such methods as in vitro fertilization, intracytoplasmic sperm injection and surrogacy. The authors have formulated proposals on the need for the state to increase funding for the assisted reproductive technology programs, when it concerns reproductive health, to allocate quotas, ensuring the availability of the technology for the enforcement of the reproductive rights in society, as well as to inform the public about new types of assisted reproductive technology. Discussion and Conclusion. The results of the study can be used by the authorities, staff of reproductive health centers and in vitro fertilization clinics to develop and plan assisted reproductive technology programs, for conducting monitoring studies on the use of assisted reproductive technology. Prospects for the study are associated with conducting further in-depth qualitative research on parents using assisted reproductive technology.

scholarly journals A Review of the Rulings by Muslim Jurists on Assisted Reproductive Technology and Reproductive Tissue Transplantation

Religions ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 720
Author(s):  
Zachariah Matthews
Keyword(s):  
Organ Donation ◽  
Assisted Reproductive Technology ◽  
Social Relationship ◽  
Reproductive Technology ◽  
Tissue Transplantation ◽  
In Vitro Fertilisation ◽  
Reproductive Tissue ◽  
And Gender ◽  
Legal Frameworks

Developments in organ donation and transplantation continue to generate controversy, especially in the field of reproductive medicine. Techniques used in assisted reproductive technology (ART), such as artificial insemination, in vitro fertilisation (IVF), gestational surrogate mothering and gender selection, continue to challenge conventional norms. The use of these techniques, as well as the transplantation of reproductive tissue such as ovaries and the testicles, for example, enables children to be conceived who may have no genetic or social relationship to one or more of their parents, biological or other. This generates religious, legal, moral and ethical dilemmas for many people, including Muslims, who tend to hold negative views about organ donation. Legal frameworks such as ijtihad (independent judgment) in conjunction with al-maqaasid al-shar’iyyah (the higher objectives of Divine decree) are assessed to review the available Sunni juristic rulings pertinent to the question, “what are the views of Muslim jurists about assisted reproductive technology and tissue transplantation given evolving implications for offspring and donors?” The review finds that a majority of Muslim jurists and juristic councils permit assisted reproductive technology and reproductive tissue transplantation with several conditions.

Assisted Reproductive Technology and Reproductive Options for the At-Risk Couple

2018 ◽  
Author(s):  
Amber Mathiesen ◽  
Kali Roy
Keyword(s):  
At Risk ◽  
In Vitro Fertilization ◽  
Assisted Reproductive Technology ◽  
Assisted Reproductive Technologies ◽  
Genetic Diagnosis ◽  
Reproductive Technologies ◽  
Reproductive Technology ◽  
Vitro Fertilization ◽  
Increased Risk

For those with an increased risk of having a child with a genetic condition, reproductive options include avoiding pregnancy altogether, undertaking prenatal diagnosis in a current pregnancy, and preventing the transmission of the genetic changes responsible for the condition to a child. This chapter on assisted reproductive technology and reproductive options for the at-risk couple describes the basic techniques of assisted reproductive technologies as well as reproductive testing options prior to in vitro fertilization, including preimplantation genetic screening (PGS) and preimplantation genetic diagnosis (PGD). It includes a brief overview of ovarian stimulation, intrauterine insemination (IUI), and in vitro fertilization. This chapter discusses the details of PGS including the process and its limitations. It also includes a discussion of PGD, including the process, such as linkage analysis, and limitations, such as allele dropout.

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