scholarly journals Retention, separation selectivity and system efficiency of selected basic psychotropic drugs on different RPLC columns

2015 ◽  
Vol 13 (1) ◽  
Author(s):  
Anna Petruczynik ◽  
Karol Wróblewski ◽  
Kamil Dzioba ◽  
Monika Waksmundzka-Hajnos
Keyword(s):  
Stationary Phase ◽  
Psychotropic Drug ◽  
Psychotropic Drugs ◽  
Stationary Phases ◽  
Acetate Buffer ◽  
System Efficiency ◽  
Organic Modifiers ◽  
Reverse Phase ◽  
Separation Selectivity ◽  
Retention Parameters

AbstractRetention parameters of psychotropic drug standards were determined on different columns, i.e., Octadecyl silica, Phenyl, Phenyl-Hexyl, Polar Reverse Phase, Pentafluorophenyl, and Cyanopropyl using aqueous eluent systems containing methanol or acetonitrile as organic modifiers, acetate buffer at pH 3.5 and addition of silanol blocker − diethylamine (DEA).The retention, separation selectivity, and sequence of elution were different when using eluents containing various organic modifiers. The significant differences were observed in retention parameters with a change of the used stationary phase. The various properties of stationary phases resulted in differences in analyte retention, peaks shape, systems efficiency and separation selectivity. The best shape of peaks were on Cyanopropyl (CN) column and the highest efficiency for most investigated psychotropic drugs were obtained on Phenyl-Hexyl and Polar RP columns.

scholarly journals Comparison of Various Chromatographic Systems for Identification of Vortioxetine in Bulk Drug Substance, Human Serum, Saliva, and Urine Samples by HPLC-DAD and LC-QTOF-MS

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2483 ◽  
Author(s):  
Anna Petruczynik ◽  
Karol Wróblewski ◽  
Krzysztof Wojtanowski ◽  
Tomasz Mroczek ◽  
Dariusz Juchnowicz ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Human Serum ◽  
Psychotropic Drugs ◽  
Biological Samples ◽  
Stationary Phases ◽  
Log P ◽  
Therapeutic Drug ◽  
Organic Modifiers ◽  
Drug Determination

Background: Determination of psychotropic drugs in clinical study is significant, and the establishment of methodologies for these drugs in biological matrices is essential for patients’ safety. The search for new methods for their detection is one of the most important challenges of modern scientific research. The methods for analyzing of psychotropic drugs and their metabolites in different biological samples should be based on combining a very efficient separation technique including high-performance liquid chromatography (HPLC), with a sensitive detection method and effectively sample preparation methods. Objective: Retention, peaks symmetry and system efficiency of vortioxetine on Hydro RP, Polar RP, HILIC A (with silica stationary phase), HILIC-B (with aminopropyl stationary phase), and ACE HILIC-N (with polyhydroxy stationary phase and SCX columns were investigated. Various mobile phases containing methanol or acetonitrile as organic modifiers and different additives were also applied to obtained optimal retention, peaks shape, and systems efficiency. The best chromatographic procedure was used for simultaneous analysis of vortioxetine and its metabolites in human serum, urine and saliva samples. Methods: Analysis of vortioxetine was performed in various chromatographic systems: Reversed phase (RP) systems on alkylbonded or phenyl stationary phases, hydrophilic interaction liquid chromatography (HILIC), and ion-exchange chromatography (IEC). Based on the dependence of log k vs the concentration of the organic modifier, log kw values for vortioxetine in various chromatographic systems were determined and compared with calculated log P values. Solid phase extraction (SPE) method was applied for sample pre-treatment before HPLC analysis. HPLC-QTOF-MS method was applied for confirmation of presence of vortioxetine and some its metabolites in biological samples collected from psychiatric patient. Conclusions: Differences were observed in retention parameters with a change of the applied chromatographic system. The various properties of stationary phases resulted in differences in vortioxetine retention, systems’ efficiency, and peaks’ shape. Lipophilicity parameters were also determined using different HPLC conditions. The most optimal systems were chosen for the analysis of vortioxetine in biological samples. Both serum and urine or saliva samples collected from patients treated with vortioxetine can be used for the drug determination. For the first time, vortioxetine was detected in patient’s saliva. Obtained results indicate on possibility of application of saliva samples, which collection are non-invasive and painless, for determination and therapeutic drug monitoring in patients.

Controllable preparation of a reverse-phase/hydrophilic interaction mixed-mode chromatographic stationary phase with adjustable selectivity

2018 ◽  
Vol 10 (45) ◽  
pp. 5387-5397 ◽  
Author(s):  
Chunmiao Bo ◽  
Xiaojun Dai ◽  
Weimin Chen ◽  
Rui Cao
Keyword(s):  
Block Copolymers ◽  
Stationary Phase ◽  
Mixed Mode ◽  
Hydrophilic Interaction ◽  
Reverse Phase ◽  
Separation Selectivity ◽  
Controllable Preparation

A mixed-mode stationary phase prepared by grafting block copolymers via ATRP could adjust separation selectivity by simply tuning the ratios of copolymer chains.

Relations between chromatographic parameters and physical properties of some alkyl tert-butyl ethers

1982 ◽  
Vol 47 (7) ◽  
pp. 1979-1987 ◽  
Author(s):  
Michal Šingliar ◽  
Vendelín Macho ◽  
Miroslav Kavala
Keyword(s):  
Gas Chromatography ◽  
Stationary Phase ◽  
Physical Properties ◽  
Homologous Series ◽  
Stationary Phases ◽  
Elution Order ◽  
Tert Butyl ◽  
Stationary Phase Polarity ◽  
Chromatographic Parameters ◽  
Retention Parameters

Retention parameters of alkyl tert-butyl ethers have been measured by gas chromatography using stationary phases of various polarity. The stationary phase polarity influences elution order of a pair alcohol/ether as well as their resolution degree. Dependence of log Vg vs boiling temperature of the ethers is linear within a given homologous series of compounds except for the first two members. The retention times of ethers and alcohols have been found to coincide when using di-n-heptyl phthalate as stationary phase.

scholarly journals Comparison of the Retention and Separation Selectivity of Aromatic Hydrocarbons with Polar Groups in RP-HPLC Systems with Different Stationary Phases and Eluents

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5070
Author(s):  
Anna Klimek-Turek ◽  
Beata Misiołek ◽  
Tadeusz H. Dzido
Keyword(s):  
Stationary Phase ◽  
Chain Length ◽  
Molecular Interactions ◽  
Aromatic Hydrocarbons ◽  
Stationary Phases ◽  
Phase Region ◽  
Separation Selectivity ◽  
Polar Groups ◽  
Rp Hplc ◽  
Mobile Phases

In this manuscript, the retention of aromatic hydrocarbons with polar groups has been compared for systems with various nonpolar columns of the types from C3 to C18 and different mobile phases composed of methanol, acetonitrile, or tetrahydrofuran as modifiers. The selectivity separation of the solutes in systems with different adsorbents, when one eluent modifier is swapped by another, has been explained, taking into account molecular interactions of the solutes with components of the stationary phase region (i.e., extracted modifier depending on the chain length of the stationary phase).

scholarly journals Optimization of chromatographic systems for analysis of selected psychotropic drugs and their metabolites in serum and saliva by HPLC in order to monitor therapeutic drugs

2019 ◽  
Vol 17 (1) ◽  
pp. 1361-1373 ◽  
Author(s):  
K. Wróblewski ◽  
A. Petruczynik ◽  
T. Tuzimski ◽  
K. Prajsnar ◽  
D. Przygodzka ◽  
...  
Keyword(s):  
Psychotropic Drugs ◽  
Solid Phase ◽  
Stationary Phases ◽  
Therapeutic Drug ◽  
Organic Modifiers ◽  
Main Metabolite ◽  
Limit Of Quantification ◽  
Therapeutic Drugs ◽  
Mobile Phases ◽  
Pre Treatment

AbstractRetention, separation selectivity and system efficiency of selected basic psychotropic drugs (clozapine, aripiprazole, vortioxetine and zolpidem) and drug metabolites (desmethylclozapine, clozapine N-oxide and dehydroaripiprazole) on Hydro RP, Phenyl-Hexyl and Polar RP columns were studied. Mobile phases containing methanol or acetonitrile as organic modifiers, acetate buffer at pH 3.5 and addition of diethylamine (DEA) as a silanol blocker were applied. Significant differences in the retention, peak shapes and systems’ efficiency of the investigated compounds were obtained depending on the tested chemically bonded stationary phases with various ligands. Based on the obtained results the Phenyl-Hexyl column was selected for analysis of the drugs and their metabolites in human serum and saliva samples. Solid phase extraction (SPE) was applied for sample pre-treatment. The best SPE-HPLC-DAD procedure was used for simultaneous analysis of clozapine, aripiprazole and their metabolites in body fluids. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method was applied for confirmation of the presence of the investigated compounds in biological samples. The lower limit of quantification (LLOQ) of clozapine obtained using the proposed method was 10 ng/mL. The validated method for determining the presence of clozapine and its main metabolite was successfully applied in therapeutic drug monitoring.

French patterns of psychotropic drug use

1990 ◽  
Vol 5 (5) ◽  
pp. 301-308
Author(s):  
V Kovess ◽  
M Ortun
Keyword(s):  
Drug Use ◽  
General Practitioners ◽  
Psychotropic Drug ◽  
Psychotropic Drugs ◽  
Elderly Population ◽  
The Elderly ◽  
Population Surveys ◽  
Minor Tranquilizers ◽  
French People ◽  
Few Data

SummaryFrench publications on psychotropics are curiously few. Data are available and French consumption of psychotropics, at least minor tranquilizers has been consistently reported as being higher than that found in other countries. The authors attempt to answer three questions: is French consuption of psychotropics really higher than other countries? Who is consuming those drugs? In which context? After having reviewed comparative data on sales, data from population surveys are analysed together with samples of prescriptions by different categories of physicians. It seems likely that French people consume more anxiolytics but this does not apply to other psychotropic drugs. Given the fact that drugs are almost free of charge in France, it is always difficult to discriminate between what has been prescribed and what has been taken. Overconsumption of anxiolytics is due to the overconsumption of the elderly population (over 60). Minor tranquilizers are mainly prescribed by general practitioners for psychological reasons.

Carboxylated cyclofructan 6 as a hydrolytically stable high efficiency stationary phase for hydrophilic interaction liquid chromatography and mixed mode separations

2016 ◽  
Vol 8 (31) ◽  
pp. 6038-6045 ◽  
Author(s):  
Yadi Wang ◽  
M. Farooq Wahab ◽  
Zachary S. Breitbach ◽  
Daniel W. Armstrong
Keyword(s):  
Liquid Chromatography ◽  
Benzoic Acid ◽  
Stationary Phase ◽  
Mixed Mode ◽  
High Efficiency ◽  
Stationary Phases ◽  
Hydrophilic Interaction Liquid Chromatography ◽  
Hydrophilic Interaction

Stationary phases composed of native cyclofructan 6 (CF6) and benzoic acid modified CF6 were synthesized and evaluated for hydrophilic interaction liquid chromatography (HILIC).

Application of Mobile Phases Containing Ionic Liquid for HPLC Analysis of Selected Isoquinoline Alkaloids

2017 ◽  
Vol 100 (6) ◽  
pp. 1652-1659 ◽  
Author(s):  
Anna Petruczynik ◽  
Justyna Misiurek ◽  
Tomasz Tuzimski ◽  
Monika Waksmundzka-Hajnos
Keyword(s):  
Ionic Liquid ◽  
Stationary Phases ◽  
Reversed Phase ◽  
System Efficiency ◽  
Organic Modifier ◽  
Optimal Conditions ◽  
Retention Behavior ◽  
Isoquinoline Alkaloids ◽  
Mobile Phases ◽  
Chemical Groups

Abstract An HPLC procedure on a polar reversed-phase column with mobile phases containing ionic liquid (IL) was developed for the analysis of selected alkaloids from different chemical groups. We aimed to obtain optimal conditions for the separation of alkaloids because widely used silica-based stationary phases exhibit a silanol effect, rendering analysis of basic analytes extremely difficult. Retention, separation selectivity, peak symmetry, and system efficiency were examined in various eluent systems containing different concentrations of IL and acetonitrile. The obtained results revealed substantial influence from the concentrations of IL, the organic modifier, and temperature on the retention behavior of the investigated alkaloids. The most selective and efficient chromatographic systems were applied for the analysis of several alkaloids in a plant extract.

PRN Medication for Psychiatric Inpatients

1987 ◽  
Vol 32 (3) ◽  
pp. 199-203 ◽  
Author(s):  
John L. Craven ◽  
Peter M. Voore ◽  
George Voineskos
Keyword(s):  
Personality Disorder ◽  
Drug Use ◽  
Psychotropic Drug ◽  
Teaching Hospital ◽  
Psychotropic Medication ◽  
Psychotropic Drugs ◽  
Psychiatric Inpatient ◽  
Psychiatric Inpatients ◽  
Psychotropic Medications ◽  
Inpatient Units

Little is known about the extent of the use of prn psychotropic medication in psychiatric inpatient units. A survey of the prn prescription and administration of psychotropic drugs in a psychiatric teaching hospital revealed that a large number of inpatients were prescribed and administered such drugs on a prn basis. Although 50% of the prescriptions were never administered, only 25% were actively discontinued by physicians. A diagnosis of personality disorder was the factor most frequently associated with the rate of prn prescriptions and of administrations. A large number of prn prescriptions had no instructions for indications, minimum time spacing between doses or maximum daily dosage. It is suggested that hospitals monitor the prn use of psychotropic medications in their inpatient units, and explore the reasons for such use. Psychotropic drug use on a prn basis should preferably be reserved for emergencies, and the instructions of prn prescriptions should be clear and detailed.

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