Application of Mobile Phases Containing Ionic Liquid for HPLC Analysis of Selected Isoquinoline Alkaloids

2017 ◽  
Vol 100 (6) ◽  
pp. 1652-1659 ◽  
Author(s):  
Anna Petruczynik ◽  
Justyna Misiurek ◽  
Tomasz Tuzimski ◽  
Monika Waksmundzka-Hajnos
Keyword(s):  
Ionic Liquid ◽  
Stationary Phases ◽  
Reversed Phase ◽  
System Efficiency ◽  
Organic Modifier ◽  
Optimal Conditions ◽  
Retention Behavior ◽  
Isoquinoline Alkaloids ◽  
Mobile Phases ◽  
Chemical Groups

Abstract An HPLC procedure on a polar reversed-phase column with mobile phases containing ionic liquid (IL) was developed for the analysis of selected alkaloids from different chemical groups. We aimed to obtain optimal conditions for the separation of alkaloids because widely used silica-based stationary phases exhibit a silanol effect, rendering analysis of basic analytes extremely difficult. Retention, separation selectivity, peak symmetry, and system efficiency were examined in various eluent systems containing different concentrations of IL and acetonitrile. The obtained results revealed substantial influence from the concentrations of IL, the organic modifier, and temperature on the retention behavior of the investigated alkaloids. The most selective and efficient chromatographic systems were applied for the analysis of several alkaloids in a plant extract.

scholarly journals Normal and reversed phase thin-layer chromatography of new 16, 17-secoestrone derivatives

2003 ◽  
Vol 68 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Marijana Acanski ◽  
Suzana Jovanovic-Santa ◽  
Lidija Jevric
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Reversed Phase ◽  
Retention Behavior ◽  
Mobile Phases ◽  
Layer Chromatography

The retention behavior and separation ability of a series of new 16,17-secoestrone derivatives has been studied on silica gel, alumina and C-18 silica gel layers with non-aqueous and aqueous-organic mobile phases. The retention behavior and separation ability are discussed in terms of the nature of the solute, eluent and stationary phase.

scholarly journals Reversed-phase thin-layer chromatography behavior of aldopentose derivatives

2012 ◽  
Vol 66 (3) ◽  
pp. 365-372 ◽  
Author(s):  
Dragana Livaja-Popovic ◽  
Eva Loncar ◽  
Lidija Jevric ◽  
Radomir Malbasa
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Stationary Phases ◽  
Reversed Phase ◽  
Chromatographic Behavior ◽  
Log P ◽  
Retention Factors ◽  
Mobile Phases ◽  
Acetone Water ◽  
Layer Chromatography

Quantitative structure-retention relationships (QSRR) have been used to study the chromatographic behavior of some aldopentose. The behavior of aldopentose derivatives was investigated by means of the reversed-phase thin-layer chromatography (RP TLC) on the silica gel impregnated with paraffin oil stationary phases. Binary mixtures of methanol-water, acetone-water and dioxane-water were used as mobile phases. Retention factors, RM0, corresponding to zero percent organic modifier in the aqueous mobile phase was determined. Lipophilicity C0 was calculated as the ratio of the intercept and slope values. There was satisfactory correlation between them and log P values calculated using different theoretical procedures. Some of these correlations offer very good predicting models, which are important for a better understanding of the relationships between chemical structure and retention. The study showed that the hydrophobic parameters RM0 and C0 can be used as a measures of lipophilicity of investigated compounds.

scholarly journals Analysis of alkaloids from different chemical groups by different liquid chromatography methods

2012 ◽  
Vol 10 (3) ◽  
pp. 802-835 ◽  
Author(s):  
Anna Petruczynik
Keyword(s):  
Liquid Chromatography ◽  
Ion Exchange ◽  
Chromatographic Separation ◽  
Mobile Phase ◽  
Stationary Phases ◽  
Biologically Active ◽  
System Efficiency ◽  
Bonded Stationary Phases ◽  
Silica Alumina ◽  
Chemical Groups

AbstractAlkaloids are biologically active compounds widely used as pharmaceuticals and synthesised as secondary methabolites in plants. Many of these compounds are strongly toxic. Therefore, they are often subject of scientific interests and analysis. Since alkaloids — basic compounds appear in aqueous solutions as ionized and unionized forms, they are difficult for chromatographic separation for peak tailing, poor systems efficiency, poor separation and poor column-to-column reproducibility. For this reason it is necessity searching of more suitable chromatographic systems for analysis of the compounds. In this article we present an overview on the separation of selected alkaloids from different chemical groups by liquid chromatography thus indicating the range of useful methods now available for alkaloid analysis. Different selectivity, system efficiency and peaks shape may be achieved in different LC methods separations by use of alternative stationary phases: silica, alumina, chemically bonded stationary phases, cation exchange phases, or by varying nonaqueous or aqueous mobile phase (containing different modifier, different buffers at different pH, ion-pairing or silanol blocker reagents). Developments in TLC (NP and RP systems), HPLC (NP, RP, HILIC, ion-exchange) are presented and the advantages of each method for alkaloids analysis are discussed.

Ethyl lactate as an environmentally friendly HPLC mobile-phase modifier in the analysis of acetaminophen, caffeine, and ASA

2013 ◽  
Vol 91 (5) ◽  
pp. 352-356 ◽  
Author(s):  
Michael D. Judge ◽  
Curtis Aab
Keyword(s):  
Acetic Acid ◽  
Mobile Phase ◽  
Environmentally Friendly ◽  
Ethyl Lactate ◽  
Core Shell ◽  
Organic Modifier ◽  
Optimal Conditions ◽  
Mobile Phases ◽  
Hplc Mobile Phase ◽  
Mobile Phase Modifier

Ethyl lactate (EL) has been investigated as an environmentally friendly organic modifier for use in HPLC mobile phases. EL shows significant promise in this regard when used under optimal conditions. In conjunction with a standard C18 column at a temperature of 60 °C, a mobile phase composed of 87% water, 10% EL, and 3% acetic acid is capable of baseline resolution of three standard pharmaceutical analytes (acetaminophen, caffeine, and acetylsalicylic acid) in under three minutes. This same mobile phase was found to be capable of extraction of these same analytes from consumer pain relief tablets with essentially 100% efficiency. Using a core-shell C18 column at 40 °C, similar resolution and run times with these analytes are possible using the same mobile phase but with a reduced EL content of only 2.5%.

scholarly journals Estimation of Solute-Stationary Phase and Solute-Mobile Phase Interactions in the Presence of Ionic Liquids

Separations ◽  
2019 ◽  
Vol 6 (3) ◽  
pp. 40
Author(s):  
María José Ruiz-Ángel
Keyword(s):  
Ionic Liquids ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Stationary Phases ◽  
Reversed Phase ◽  
Retention Mechanism ◽  
Chromatographic Behavior ◽  
Chemical Equilibria ◽  
Suppression Effect ◽  
Mobile Phases

The presence of free silanols on alkyl-bonded reversed-phase stationary phases is responsible for broad and asymmetrical peaks when basic drugs are chromatographed with conventional octadecylsilane (C18) columns due to ionic interactions. In the last few years, ionic liquids (ILs) have attracted attention to reduce this undesirable silanol activity. ILs should be considered as dual modifiers (with a cationic and anionic character), which means that both cations and anions are able to adsorb on the stationary phase, creating a positively or negatively charged layer, depending on the relative adsorption. The accessibility of basic compounds to the silanols is prevented by both the IL cation and anion, improving the peak profiles. A comparative study of the performance of six imidazolium-based ILs, differing in their cation/anions, as modifiers of the chromatographic behavior of a group of ten β-adrenoceptor antagonists, is addressed. Mobile phases containing cationic amines (triethylamine and dimethyloctylamine) were used as a reference for the interpretation of the results. Using a mathematical model based on two chemical equilibria, the association constants between the solutes and modified stationary phase as well as those between solutes and the additive in the mobile phase were estimated. These values, together with the changes in retention and peak shape, were used to obtain conclusions about the retention mechanism, changes in the nature of the chromatographic system, and silanol suppression effect.

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