Association of genetic polymorphisms in vitamin D receptor (ApaI, TaqI and FokI) with vitamin D and glycemic status in type 2 diabetes patients from Southern India

Abstract Objectives To evaluate the association of VDR polymorphisms (FokI, TaqI and ApaI) with vitamin D levels and glycemic status in type 2 diabetes patients from Southern India. Methods In this observational study, genotype frequencies and vitamin D levels of 200 cases (type 2 diabetes patients) were compared with 300 controls (unrelated anonymised stored samples of healthy volunteers) from south India. Serum 25 (OH) D levels were measured by immunoassay technique, glycated hemoglobin (HbA1c) was measured using HPLC and genotyping of VDR polymorphisms were carried out using Real time Polymerase Chain Reaction (RT PCR). Results About 69.2% of type 2 diabetes patients were found to have vitamin D deficiency. FokI polymorphism showed variations in serum 25 (OH) D levels, with AA and AG genotypes having significantly lower serum 25 (OH) D levels as compared to GG [13.24 (8.4) ng/ml, 15.02 (7.07) ng/ml and 20.67 (13.64) ng/ml respectively]. There was no difference in HbA1c levels with respect to the vitamin D levels and VDR polymorphisms. Conclusions AA and AG genotypes of FokI polymorphisms are associated with low serum 25 (OH) D levels. However there was no association between VDR polymorphisms and glycemic status in south Indian type 2 diabetes patients.

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Lack of Correlation of the Serum 25(OH) Vitamin D Levels with the Glycated Hemoglobin A1c and the Lipid Profile in Type 2 Diabetes Patients on Oral Antidiabetic Drugs – Preliminary Data

Acta Medica Bulgarica ◽

10.1515/amb-2014-0015 ◽

2014 ◽

Vol 41 (2) ◽

pp. 12-19 ◽

Cited By ~ 1

Author(s):

D. Bakalov ◽

M. Boyanov ◽

A. Tsakova

Keyword(s):

Type 2 Diabetes ◽

Vitamin D ◽

Hemoglobin A1c ◽

Glycated Hemoglobin ◽

Glycated Hemoglobin A1c ◽

Vitamin D Levels ◽

The Mean ◽

25 Oh Vitamin D ◽

Diabetes Patients

Summary Data from different studies correlating the serum 25(OH)D levels with the metabolic and glycemic parameters in type 2 diabetes patients are still varying. The objective if this study was to describe the correlation between serum 25(OH)D levels and some metabolic parameters in Bulgarian type 2 diabetes patients on oral antidiabetic drugs. One hundred type 2 diabetes patients participated - 56 men and 44 women. The mean age and diabetes duration of the women was 59.0 and 9.8 years, of the men - 58.0 and 7.7 years respectively. Complete patient history was taken and physical examination was performed (body weight and height, waist circumference). Body composition was measured on a leg-to-leg body impedance analyzer (TBF-215, Tanita Corp., Tokyo, Japan). Serum levels of vitamin D were measured by electro-hemi-luminescent detection as 25-(ОН) D Total (ECLIA, Elecsys 2010, Roche Diagnostics, Switzerland). Glycated hemoglobin A1c was measured on a NycoCard reader (Alere™). Total, HDL-cholesterol (direct) and triglycerides were analyzed on a Cobas Integra 400+ analyzer. Correlation analysis was performed on a SPSS 13.0 for Windows platform and included 10 curves. The data were first analyzed for the group as a whole and then separately for men and women as well as in the different vitamin D tertiles. The mean serum 25-OH-vitamin D levels were 23.8 ± 12.1 nmol/l in women and 33.3 ± 20.0 nmol/l in men. We were unable to find any statistically significant correlation between serum 25(OH) vitaminand the serum lipids (cholesterol profile and triglycerides). On the contrary, there was a weak correlation with the glycated hemoglobin A1c (cubic model, R2 = 0.178, p = 0.05) and the BMI (inverse model, R2 = 0.101, p = 0.038). The sub-analyses (men versus women or according to tertiles of vitamin D) did not produce any additional information. The influence of vitamin D on the parameters of the metabolic control in type 2 diabetes is very weak on an individual level. It might be only demonstrated in large epidemiological surveys.

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Vitamin D Daily short-term Supplementation does not Affect Glycemic Outcomes of Patients with Type 2 Diabetes

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000239 ◽

2016 ◽

Vol 86 (5-6) ◽

pp. 169-183 ◽

Cited By ~ 1

Author(s):

Stavri Chrysostomou

Keyword(s):

Type 2 Diabetes ◽

Vitamin D ◽

Vitamin D Supplementation ◽

Inverse Association ◽

Glycemic Status ◽

Positive Effects ◽

Vitamin D Levels ◽

Glycemic Outcomes ◽

The Impact

There is currently insufficient evidence of a beneficial effect to recommend vitamin D supplementation for optimizing glycemic status in patients with type 2 diabetes mellitus (T2DM). Taking into consideration the significant extra-skeletal effect of vitamin D on pancreatic β-cell function and insulin secretion and the large number of scientific evidence supporting the inverse association between vitamin D status and hyperglycemia, this review article aims to examine whether vitamin D supplementation therapies are beneficial to patients with T2DM considering specific factors through randomized controlled trials (RCTs). EBSCOhost and Medline databases were searched from the beginning of 2009 until the end of 2014 for RCTs in patients with T2DM. Parameters, such as baseline vitamin D levels, frequency/dosage of supplementation, length of the study and type of supplementation, were independently assessed, based on their effect on glycemic status. Although all different types of supplementation were safe and effective in the achievement of vitamin D sufficiency in a dose-dependent way, the impact on glycemic status was different. 14 RCTs were included with daily supplementations ranging from 400-11.200 IU/daily, 40.000-50.000 IU/weekly and 100.000-300.000 IU/intramuscularly or once given, for a period from 8 to 24 weeks. Daily supplementation of vitamin D (up to 11.200 IU) showed no effect, whereas combined supplementation, with calcium (≥300 mg), and with vitamin D doses similar to the RDA, showed positive effects. Additionally, high weekly doses of vitamin D (40.000-50.000 IU) were effective on glycemic outcomes but available data are limited.

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Lower Vitamin D Levels, but Not VDR Polymorphisms, Influence Type 2 Diabetes Mellitus in Brazilian Population Independently of Obesity

Medicina ◽

10.3390/medicina55050188 ◽

2019 ◽

Vol 55 (5) ◽

pp. 188 ◽

Cited By ~ 1

Author(s):

Kathryna Fontana Rodrigues ◽

Nathalia Teixeira Pietrani ◽

Adriana Aparecida Bosco ◽

Maira Cândida Rodrigues de Sousa ◽

Ieda de Fátima Oliveira Silva ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Vitamin D ◽

Type 2 Diabetes Mellitus ◽

Gene Polymorphisms ◽

Vdr Gene ◽

Vitamin D Levels ◽

Vdr Gene Polymorphisms ◽

Vdr Polymorphisms

Background and Objectives: Vitamin D levels have been associated with a diversity of diseases, including obesity. Vitamin D presents a pleiotropic action, and can regulate insulin secretion and inflammatory responses. Vitamin D receptor (VDR) gene polymorphisms are involved in the gene expression regulation and have been associated with type 2 diabetes mellitus (T2DM). This study aimed to evaluate the association between the polymorphisms ApaI (rs7975232), BsmI (rs1544410), FokI (rs10735810), and TaqI (rs731236) in the VDR gene in people diagnosed with T2DM, and plasma 25-hydroxivitamin D levels [25(OH)D]. Materials and Methods: A total of 101 T2DM patients and 62 gender, age, and body mass index (BMI) matched non-diabetic controls were included in this study. Molecular analyzes were performed by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP). The plasma 25(OH)D levels were measured by high performance liquid chromatography. Results: The plasma 25(OH)D levels were lower in T2DM patients (17.2 (16.6) ng/mL) when compared with the control subjects (30.8 (16.2) ng/mL, p < 0.0001), independently of obesity status. We found no difference between genotypic and allelic frequencies of the VDR polymorphisms when comparing the T2DM group and control group (p > 0.05 for all), and did not show any association with plasma 25(OH)D levels. Conclusions: These results suggest that T2DM is associated with lower plasma 25(OH)D levels, which are not related to BMI and VDR gene polymorphisms.

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Assessment of vitamin D levels in type 1 and type 2 diabetes patients: Results from metaanalysis

Molecular Nutrition & Food Research ◽

10.1002/mnfr.201500937 ◽

2016 ◽

Vol 60 (5) ◽

pp. 1059-1067 ◽

Cited By ~ 29

Author(s):

Liang Shen ◽

Qi-Shuai Zhuang ◽

Hong-Fang Ji

Keyword(s):

Type 2 Diabetes ◽

Vitamin D ◽

Vitamin D Levels ◽

Diabetes Patients

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Low serum vitamin D levels in type 2 diabetes patients are associated with decreased mycobacterial activity

BMC Infectious Diseases ◽

10.1186/s12879-017-2705-1 ◽

2017 ◽

Vol 17 (1) ◽

Cited By ~ 7

Author(s):

María Teresa Herrera ◽

Yolanda Gonzalez ◽

Fernando Hernández-Sánchez ◽

Guadalupe Fabián-San Miguel ◽

Martha Torres

Keyword(s):

Type 2 Diabetes ◽

Vitamin D ◽

Serum Vitamin ◽

Serum Vitamin D ◽

Vitamin D Levels ◽

Low Serum ◽

Diabetes Patients

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Abstract #421 Association of Vitamin D Levels with the Severity of Fibrosis in NAFLD Patients with Type 2 Diabetes

Endocrine Practice ◽

10.1016/s1530-891x(20)46765-5 ◽

2019 ◽

Vol 25 ◽

pp. 199-200

Author(s):

Prathyusha Chitrapu ◽

Shilpa Jain ◽

Aaron Thrift ◽

Maya Balakrishnan ◽

Ruchi Gaba

Keyword(s):

Type 2 Diabetes ◽

Vitamin D ◽

Vitamin D Levels

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Abstract #251: Prevalence of Vitamin D Deficiency in Type 2 Diabetes Patients in North Indian Population and its Correlation with Diabetic Complications and Comorbidities

Endocrine Practice ◽

10.1016/s1530-891x(20)44959-6 ◽

2016 ◽

Vol 22 ◽

pp. 56-57

Author(s):

Brij Makkar ◽

Namrata Harichandan ◽

Jugal Sharma ◽

Rajeev Chawla ◽

Shalini Jaggi ◽

...

Keyword(s):

Type 2 Diabetes ◽

Vitamin D ◽

Vitamin D Deficiency ◽

Diabetic Complications ◽

Indian Population ◽

North Indian Population ◽

Diabetes Patients

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Reduced Lower-Limb Muscle Strength and Volume in Patients With Type 2 Diabetes in Relation to Neuropathy, Intramuscular Fat, and Vitamin D Levels

Diabetes Care ◽

10.2337/dc15-0995 ◽

2016 ◽

Vol 39 (3) ◽

pp. 441-447 ◽

Cited By ~ 56

Author(s):

Monirah M. Almurdhi ◽

Neil D. Reeves ◽

Frank L. Bowling ◽

Andrew J.M. Boulton ◽

Maria Jeziorska ◽

...

Keyword(s):

Type 2 Diabetes ◽

Vitamin D ◽

Muscle Strength ◽

Lower Limb ◽

Intramuscular Fat ◽

Lower Limb Muscle ◽

Limb Muscle ◽

Vitamin D Levels

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The role of vitamin D in the development of type 2 diabetes

Voprosy detskoj dietologii ◽

10.20953/1727-5784-2021-1-44-52 ◽

2021 ◽

Vol 19 (1) ◽

pp. 44-52

Author(s):

A.P. Shumilov ◽

◽

M.Yu. Semchenkova ◽

D.S. Mikhalik ◽

T.G. Avdeeva ◽

...

Keyword(s):

Insulin Resistance ◽

Type 2 Diabetes ◽

Vitamin D ◽

Inverse Relationship ◽

Biological Mechanisms ◽

Β Cell ◽

Cell Dysfunction ◽

Vitamin D Levels

Vitamin D plays an important role in decreasing the risk of developing type 2 diabetes by influencing calcium metabolism, thereby reducing β-cell dysfunction and preventing insulin resistance. The findings of research works are contradictory enough, although some of them demonstrated an inverse relationship between vitamin D levels and the incidence of type 2 diabetes. The article describes the biological mechanisms of relationships between vitamin D levels and type 2 diabetes, reviews the results of the studies conducted and summarizes the available data. Key words: vitamin D, type 2 diabetes mellitus, insulin resistance

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