scholarly journals Evaluation of the Genotoxic Effect of the Commercial Fungicide Tango® Super on Bovine Lymphocytes

2017 ◽  
Vol 61 (4) ◽  
pp. 28-33
Author(s):  
M. Grajciarová ◽  
B. Holečková
Keyword(s):  
Dna Fragmentation ◽  
Cytogenetic Analysis ◽  
Donor Cell ◽  
Electrophoretic Analysis ◽  
Genotoxic Effects ◽  
Conventional Cytogenetic Analysis ◽  
Last Stage ◽  
Bovine Lymphocytes ◽  
Commercial Fungicide

AbstractThis study investigated the potential genotoxic effects of the fungicide Tango®Super using methods of conventional cytogenetic analysis, fluorescence in situ hybridization (FISH) and detection of DNA fragmentation in bovine lymphocytes. After exposure of two donor cell cultures to several concentrations of fungicide (0.5, 3.0 and 15.0 mg.ml-1for conventional cytogenetic analysis; 0.5 and 3.0 mg.ml-1for FISH) we detected the insignificant occurrence of chromosome and chromatid breakages. In both donors we observed a significant decrease in mitotic index (MI) percentage with increasing concentrations of fungicide (P < 0.01; P < 0.001), which indicated a cytotoxic effect of the preparation. Electrophoretic analysis of DNA fragmentation in lymphocytes exposed to increasing concentrations (0.5; 1.5; 3.0; 6.0 and 15.0 mg.ml-1) of this preparation showed its ability to induce formation of fragments, which is a characteristic manifestation of the last stage of apoptosis.

scholarly journals Testing the Potential Clastogenic/Cytotoxic Effects of Pesticide CALYPSO 480 SC

2017 ◽  
Vol 61 (3) ◽  
pp. 47-51 ◽  
Author(s):  
I. Ficová ◽  
M. Galdíková
Keyword(s):  
Chromosome Analysis ◽  
Chromosome Breakage ◽  
Commercial Preparation ◽  
Cytotoxic Effects ◽  
Conventional Cytogenetic Analysis ◽  
Structural Aberrations ◽  
Bovine Lymphocytes ◽  
Genotoxic Action

AbstractThe detection of chromosomal damage serves as a tool for the verification of the genotoxic effects of chemical substancesin vitro. We used conventional cytogenetic analysis in order to test for the potential genotoxic action of the insecticide thiacloprid (the active ingredient in commercial preparation CALYPSO 480 SC). The test cultures of bovine lymphocytes obtained from the peripheral blood were incubated with the insecticide in concentrations of: 30, 120, 240 and 480 μg.ml−1for 24 and 48 hours. After 24 hours of incubation, we observed that the increasing concentrations resulted in a significant (P < 0.05; P < 0.01) increase in the frequency of DNA damage. Our experiments showed the presence of aberrations of a non-stable type (chromatid and chromosome breakage). The conventional chromosome analysis was supplemented with fluorescencein situhybridization for the detection of numeric and stable structural aberrations. Whole chromosome probes for bovine chromosomes 1, 5 and 7 (BTA 1, BTA 5 and BTA 7) were used in the experiments.

Striking Coincidence of IgD M-Protein and Translocation t(11;14) in Hungarian Myeloma Patients.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 5046-5046
Author(s):  
Gabor Mikala ◽  
Emma Adam ◽  
Andras Kozma ◽  
Balazs Kapas ◽  
Sarolta Nahajevszky ◽  
...  
Keyword(s):  
Cyclin D1 ◽  
Cytogenetic Analysis ◽  
Fluorescent In Situ Hybridization ◽  
Biological Properties ◽  
The Other ◽  
M Protein ◽  
Conventional Cytogenetic Analysis ◽  
Igh Locus ◽  
Number Of Patients

Abstract Multiple myeloma exhibiting production of IgD M-protein is a rare subset (appr. 1 %) of all patients. Translocation t(11;14) is, on the other hand, the most common primary translocation in myeloma that involves the IgH locus and cyclin D1, approximately 16–22% of all patients harbor this genetic rearrangement. This cytogenetic abnormality has been previously associated with nonsecretory behavior and also with production of IgE M-protein. Out of 317 myeloma patients treated in our department in 2004–2006, five patients had IgD M-protein (four belonged to IgD-lambda and one to IgD kappa subtype). Cytogenetic analysis by interphase fluorescent in situ hybridization (FISH) revealed the presence of the IgH/Cyclin D1 abnormality in all five of them (100%), while the prevalence of this translocation was 17% in the entire myeloma population seen by our ward and tested by FISH (132 patients). One IgD patient had a complex IgH/MYC/CyclinD1 translocation, also confirmed by conventional cytogenetic analysis of metaphases, while two of five patients had additional copies of IgH and Cyclin D1 genes, possibly indicating atypical translocations and/or hyperdiploidy. Although the number of patients with this rare disease in our report is low (5), we find the uniform presence of translocation t(11;14) a striking occurrence that may explain some of the unusual biological properties of this myeloma subtype.

Genomic in situ hybridization (GISH) reveals high chromosome pairing affinity between Lolium perenne and Festuca mairei

Genome ◽  
2000 ◽  
Vol 43 (2) ◽  
pp. 398-403 ◽  
Author(s):  
Mingshu Cao ◽  
David A Sleper ◽  
Fenggao Dong ◽  
Jiming Jiang
Keyword(s):  
In Situ Hybridization ◽  
Lolium Perenne ◽  
Chromosome Pairing ◽  
Cytogenetic Analysis ◽  
Direct Evidence ◽  
Genomic In Situ Hybridization ◽  
Genomic Constitution ◽  
Conventional Cytogenetic Analysis ◽  
Festuca Mairei

Intergeneric hybridizations have been made between species of Lolium and Festuca. It has been demonstrated, largely through conventional cytogenetic analysis, that the genomes of the two genera are related, however, much information is lacking on exactly how closely related the genomes are between the two species. We applied genomic in situ hybridization (GISH) techniques to the F1 hybrids of tetraploid Festuca mairei with a genomic constitution of M1M1M2M2 and diploid Lolium perenne with a genomic constitution of LL. It was shown in the triploid hybrids (LM1M2) that the chromosomes of M1 and M2 from F. mairei could pair with each other, and it was further discovered that L chromosomes of L. perenne paired with M1 and M2 chromosomes. Our results showed that meiocytes of Lolium-Festuca are amenable to GISH analysis, and provided direct evidence for the hypothesis that the chromosomes of Lolium and Festuca may be genetically equivalent and that reciprocal mixing of the genomes may be possible. Key words: Lolium, Festuca, in situ hybridization, meiosis.

Mature B-Cell Leukemias With More Than 55% Prolymphocytes

2003 ◽  
Vol 127 (3) ◽  
pp. 305-309
Author(s):  
Shakil Merchant ◽  
Ellen Schlette ◽  
Warren Sanger ◽  
Raymond Lai ◽  
L. Jeffrey Medeiros
Keyword(s):  
In Situ Hybridization ◽  
B Cell ◽  
Fluorescence In Situ Hybridization ◽  
Cytogenetic Analysis ◽  
Lymphocytic Leukemia ◽  
Chromosomal Translocations ◽  
Prolymphocytic Leukemia ◽  
Conventional Cytogenetic Analysis ◽  
Myc Gene

Abstract Context.—The molecular genetic events involved in the pathogenesis of mature B-cell leukemias with more than 55% prolymphocytes are not well characterized. We have encountered 2 such cases in which conventional cytogenetic analysis identified Burkitt lymphoma–type chromosomal translocations involving 8q24. Objective.—To assess these 2 cases for involvement of the c-myc gene using fluorescence in situ hybridization analysis with probes specific for the c-myc and immunoglobulin heavy-chain (IgH) genes. Results.—In both cases, conventional cytogenetic analysis demonstrated complex karyotypes, including chromosomal translocations involving 8q24. In case 1, a case of de novo prolymphocytic leukemia, the t(8;14)(q24;q32) was detected. In case 2, a case of chronic lymphocytic leukemia in prolymphocytoid transformation, the t(8;22)(q24;q11) was identified. Fluorescence in situ hybridization studies showed c-myc/IgH fusion signals in case 1, proving the presence of the t(8;14). Split c-myc signals without fusion to IgH were observed in case 2, proving c-myc gene rearrangement and consistent with the t(8;22). Conclusion.—These results suggest that c-myc gene alterations may be involved in the pathogenesis of a subset of mature B-cell leukemias with more than 55% prolymphocytes.

scholarly journals A Dual Gender Rare Case with 47,XY, + 18/46,XX Karyotype: Chimera or Mosaic?

2021 ◽  
Vol 11 (01) ◽  
pp. e41-e44
Author(s):  
Ravindran Ankathil ◽  
Foong Eva ◽  
Zulaikha Abu Bakar ◽  
Nazihah Mohd Yunus ◽  
Nurul Alia Nawi ◽  
...  
Keyword(s):  
Birth Weight ◽  
Blood Sample ◽  
Cell Line ◽  
Apgar Score ◽  
Cytogenetic Analysis ◽  
Rare Case ◽  
Trisomy 18 ◽  
Normal Female ◽  
Conventional Cytogenetic Analysis ◽  
Edwards Syndrome

Our objective is to report one rare case of dual gender chimerism involving abnormal male trisomy 18 and normal female karyotype. The baby was born full term with birth weight of 1.8 kg, not vigorous with light meconium stained liquor and Apgar score of 51, 85 and 910. Parents are 40 years old and mother is G6P5 + 1. The baby had clinical features of Edwards syndrome, and a blood sample was sent to Human Genome Centre, Universiti Sains Malaysia, Malaysia for cytogenetic analysis. Conventional cytogenetic analysis results showed two distinct sex discordant genetic cell lines XY and XX in 90:10 ratio. The male genetic cell line XY also showed trisomy 18 (47,XY, + 18) consistent with clinical diagnosis of male Edwards syndrome, whereas the second genetic cell line showed normal 46,XX female. The present case was reported as dual gender chimera with chi 47,XY, + 18/46,XX karyotype pattern. To the best of available knowledge, dual gender chimerism with abnormal male trisomy 18 and normal female karyotype has not been reported so far, and this case is reported for its rarity and as the first report.

Cytogenetic analysis of prostate adenocarcinomas by interphase DNA in situ hybridization

1994 ◽  
Vol 77 (2) ◽  
pp. 194
Author(s):  
J.C. Alers ◽  
P.J. Krijtenburg ◽  
F.T. Bosman ◽  
Th.H. van der Kwast ◽  
H. van Dekken
Keyword(s):  
In Situ Hybridization ◽  
Cytogenetic Analysis
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