scholarly journals First record and molecular identification of Toxocara cati in a Pallas’ cat Otocolobus manul from Kyrgyzstan

2016 ◽  
Vol 53 (3) ◽  
pp. 281-284 ◽  
Author(s):  
M. Heddergott ◽  
K. Zhumabai Uulu ◽  
A.N. Barashkova ◽  
A.C. Frantz
Keyword(s):  
Molecular Identification ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Morphological Characteristics ◽  
First Record ◽  
Toxocara Cati ◽  
Conservation Implication ◽  
In The Wild ◽  
Second Internal Transcribed Spacer ◽  
Genetic Barcoding

Summary In the present paper, we the report the first documented occurrence in the wild of Toxocara cati in the sole representative of the genus Otocolobus, the Pallas’ cat. The identity of the parasite was confirmed by morphological characteristics and by genetic barcoding of the second internal transcribed spacer of ribosomal DNA. The morphological measures of the T. cati specimens from the Kyrgyz Pallas’ cat were comparable to values reported. We discuss the conservation implication of our find.

scholarly journals Intragenomic variation in the second internal transcribed spacer of the ribosomal DNA of species of the genera Culex and Lutzia (Diptera: Culicidae)

2011 ◽  
Vol 106 (1) ◽  
pp. 01-08 ◽  
Author(s):  
Fabiana Tavares Vesgueiro ◽  
Bruna Demari-Silva ◽  
Rosely dos Santos Malafronte ◽  
Maria Anice Mureb Sallum ◽  
Mauro Toledo Marrelli
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Intragenomic Variation ◽  
Second Internal Transcribed Spacer

scholarly journals Sequences of the second internal transcribed spacer of ribosomal DNA for three species of Trichostrongylus (Nematoda: Trichostrongylidae) from sheep in Russia

2007 ◽  
Vol 44 (2) ◽  
pp. 43-46 ◽  
Author(s):  
D. Kuznetsov ◽  
N. Kuznetsova
Keyword(s):  
Dna Sequence ◽  
Intraspecific Variation ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Moscow Region ◽  
Dna Sequence Data ◽  
Interspecific Differences ◽  
Second Internal Transcribed Spacer ◽  
First Time

AbstractFor the first time, DNA sequence data were obtained for three species of Trichostrongylus from Russia. Internal transcribed spacer (ITS-2) of ribosomal DNA was sequenced for T. axei, T. colubriformis and T. probolurus from sheep from the Moscow region. ITS-2 rDNA length was estimated as 238 nucleotides for T. colubriformis and T. probolurus and 237 nucleotides for T. axei. The G+C content of the ITS-2 sequences of T. colubriformis, T. axei and T. probolurus were 31 %, 32 % and 34 % respectively. The level of interspecific differences in ITS-2 of rDNA of T. axei, T. probolurus and T. colubriformis ranged from 3 to 4 %. The ITS-2 sequences from the Russian specimens were compared with those of T. axei, T. probolurus and T. colubriformis from Australia and Germany. Intraspecific variation ranged from 0 % in T. colubriformis to 3.0 % in T. axei.

Identification of Closely Related Hydrobaenus Species (Diptera: Chironomidae) Using the Second Internal Transcribed Spacer (ITS2) Region of Ribosomal DNA

2004 ◽  
Vol 26 (3-4) ◽  
pp. 207-213 ◽  
Author(s):  
Hidetake Asari ◽  
Shiro Kasuya ◽  
Tadashi Kobayashi ◽  
Shigeo Kondo ◽  
Isao Nagano ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Its2 Region ◽  
Second Internal Transcribed Spacer

scholarly journals A new subfamily for a clade of opecoelids (Trematoda: Digenea) exploiting marine fishes as second-intermediate hosts, with the first report of opecoelid metacercariae from an elasmobranch

2019 ◽  
Author(s):  
Storm Blas Martin ◽  
Abigail Jayne Downie ◽  
Thomas Herbert Cribb
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Marine Fishes ◽  
Barrier Reef ◽  
Intermediate Hosts ◽  
Internal Transcribed Spacer Region ◽  
Epaulette Shark ◽  
The Family ◽  
Unidentified Species ◽  
Second Internal Transcribed Spacer

Abstract Metacercariae of trematodes belonging to the family Opecoelidae were collected from small fishes of the Great Barrier Reef: a blenniid, two gobiids, two labrids, three pomacentrids, a monacanthid, an ostraciid and the epaulette shark, Hemiscyllium ocellatum. Sequences of the second internal transcribed spacer region (ITS2) of ribosomal DNA were generated from these metacercariae in an attempt to match them with adult worms. Three species of Allopodocotyle (Allopodocotyle epinepheli, Allopodocotyle heronensis and an unidentified species), two unidentified species of Hamacreadium and Pacificreadium serrani were detected. Among the Opecoelidae, these species all resolve to a single, phylogenetically and somewhat morphologically distinct clade. Species of this clade are the only known marine opecoelids to exploit fishes as second-intermediate hosts. The clade is proposed to warrant a new subfamily, the Hamacreadiinae subfam. nov. It includes Allopodocotyle, Bentholebouria, Cainocreadium, Choanotrema, Hamacreadium, Pacificreadium, Paraplagioporus, Pedunculacetabulum and Podocotyloides.

Sequence Analysis of the Second Internal Transcribed Spacer of Ribosomal DNA in Anopheles oswaldoi (Diptera: Culicidae)

1999 ◽  
Vol 36 (6) ◽  
pp. 679-684 ◽  
Author(s):  
Mauro T. Marrelli ◽  
Rosely S. Malafronte ◽  
Carmen Flores-Mendoza ◽  
Ricardo Lourenço-De-Oliveira ◽  
Judith K. Kloetzel ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Second Internal Transcribed Spacer
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