Mitochondrial DNA Characterization of High Royal Jellyproducing Honeybees (Hymenoptera: Apidae) in China

Abstract China is the largest producer and exporter of royal jelly in the world. The high production of royal jelly in China is mainly attributed to a high royal jelly-producing lineage of honeybees (Apis mellifera) (HRJB). However, few studies have been conducted on the genetic characterization of HRJB. In this study, the mitochondrial DNA intergenic region between cytochrome oxidase I and II (COI-COII) and the mitochondrial NADH dehydrogenase subunit 2 sequences (ND2) were determined for 90 HRJB colonies, collected from the regions of China where HRJB originated, and 25 unimproved A. m. ligustica colonies from China. COI-COII sequence analysis revealed two mitotypes (C1 and C2d) in HRJB colonies and one mitotype (C1) in unimproved A. m. ligustica colonies. The main mitotype (C1) in HRJB accounted for 93% of the colonies. Based on ND2 sequences, four and two mitotypes were found in HRJB and unimproved A. m. ligustica colonies, respectively. Sequence alignment showed that nucleotides in three positions of the ND2 sequence were different between the main mitotype of HRJB and that of unimproved A. m. ligustica. Our study suggested that HRJB was bred from A. m. ligustica and possibly had genetic characteristics different from unimproved A. m. ligustica.

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Genetic characterization of parthenogenic Fasciola sp. in Japan on the basis of the sequences of ribosomal and mitochondrial DNA

Parasitology ◽

10.1017/s0031182005008292 ◽

2005 ◽

Vol 131 (5) ◽

pp. 679-685 ◽

Cited By ~ 97

Author(s):

T. ITAGAKI ◽

M. KIKAWA ◽

K. SAKAGUCHI ◽

J. SHIMO ◽

K. TERASAKI ◽

...

Keyword(s):

Mitochondrial Dna ◽

Nadh Dehydrogenase ◽

Genetic Characterization ◽

Interspecific Cross ◽

Accurate Identification ◽

Cross Hybridization ◽

Morphological Variations ◽

Liver Flukes ◽

Its1 And Its2

Accurate identification of aspermic Fasciola forms in Japan remains difficult because of their morphological variations. In order to characterize the forms genetically, nucleotide sequences of ribosomal internal transcribed spacer (ITS1 and ITS2) and mitochondrial cytochrome c oxidase I (COI) and NADH dehydrogenase I (NDI) genes in 34 liver flukes from 16 prefectures in Japan were analysed. Two major forms represented by Fsp 1 and Fsp 2 had sequences identical to or closely resembling those of F. hepatica and F. gigantica, respectively, in all the 4 DNA markers and were mainly distributed in northern and eastern-western parts of Japan, respectively. Fsp 1 and Fsp 2 would have been introduced into Japan with infected cattle of 2 distinct lineages via the Korean Peninsula and spread through limited parts of Japan (northern and eastern-western parts) together with the movement of each cattle lineage. The Japanese form (Fsp 1/2), which showed heterozygosity in ribosomal DNA and Fsp 2 haplotype in mitochondrial DNA, may have originated in interspecific cross hybridization between paternal F. hepatica and maternal F. gigantica.

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Genetic characterization of Phytophthora nicotianae by the analysis of polymorphic regions of the mitochondrial DNA

Fungal Biology ◽

10.1016/j.funbio.2011.02.018 ◽

2011 ◽

Vol 115 (4-5) ◽

pp. 432-442 ◽

Cited By ~ 18

Author(s):

Marco Antonio Mammella ◽

Santa Olga Cacciola ◽

Frank Martin ◽

Leonardo Schena

Keyword(s):

Mitochondrial Dna ◽

Genetic Characterization ◽

Phytophthora Nicotianae

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Genetic characterization of Vietnamese Yellow cattle using mitochondrial DNA and Y-chromosomal haplotypes and genes associated with economical traits

Animal Science Journal ◽

10.1111/asj.13099 ◽

2018 ◽

Vol 89 (12) ◽

pp. 1641-1647

Author(s):

Thu N. A. Le ◽

Hai V. Vu ◽

Yu Okuda ◽

Huong T. Duong ◽

Trung B. Nguyen ◽

...

Keyword(s):

Mitochondrial Dna ◽

Genetic Characterization ◽

Yellow Cattle

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Genetic characterization of cystic pulmonary echinococcosis in Sudan as determined by mitochondrial DNA sequences

Journal of Surgery and Research ◽

10.26502/jsr.10020145 ◽

2021 ◽

Vol 04 (03) ◽

Author(s):

Sara S Abdalla ◽

Mohamed E Ahmed ◽

Alsmawal Awad Elimam ◽

Mawahib H Eldigail ◽

Imadeldin E Aradaib ◽

...

Keyword(s):

Mitochondrial Dna ◽

Dna Sequences ◽

Genetic Characterization ◽

Pulmonary Echinococcosis

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Genetic Characterization of Kazakh Native Sheep Breeds Using Mitochondrial DNA

OnLine Journal of Biological Sciences ◽

10.3844/ojbsci.2018.341.348 ◽

2018 ◽

Vol 18 (3) ◽

pp. 341-348 ◽

Cited By ~ 1

Author(s):

Ilmira Mukhametzharova ◽

Yessenbay Islamov ◽

Saukymbek Shauyenov ◽

Dulat Ibrayev ◽

Sabina Atavliyeva ◽

...

Keyword(s):

Mitochondrial Dna ◽

Genetic Characterization ◽

Sheep Breeds ◽

Native Sheep

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Cryptosporidium in asymptomatic children in Southern Xinjiang, China and the potential of zoonotic transmission

10.21203/rs.3.rs-551246/v1 ◽

2021 ◽

Author(s):

Tian Wang ◽

Zilin Wei ◽

Ying Zhang ◽

Qiyuan Zhang ◽

Longxian Zhang ◽

...

Keyword(s):

Infection Rate ◽

Maximum Likelihood Method ◽

Genetic Characterization ◽

Pcr Amplification ◽

Likelihood Method ◽

Limited Information ◽

Genetic Characteristics ◽

Asymptomatic Children ◽

Southern Xinjiang

Abstract Background: Cryptosporidium is a primary cause of diarrhea in children globally. However, there is limited information on the prevalence and genetic characterization of Cryptosporidium in children in Xinjiang, China. This study aimed to assess the genetic characteristics and epidemiological status of Cryptosporidium in asymptomatic children in Southern Xinjiang, China.Methods: A total of 609 fecal samples were collected from kindergartners aged 2-6 y from 11 counties of Southern Xinjiang, China. We used nested PCR amplification of partial SSU rDNA gene to screen the samples for Cryptosporidium spp. The isolates containing C. parvum and C. hominis were further subtyped by a 60-kDa glycoprotein (gp60). We used MEGA7 to construct a phylogenetic tree to study the genetic relationship between the gp60 subtypes of these two species via the Maximum Likelihood method based on the Tamura-Nei model.Results: Only 1.3% (8/609) of asymptomatic children were confirmed as infected with Cryptosporidium with 2.0% (6/299) infection rate in boys and 0.6% (2/310) infection rate in girls. Three Cryptosporidium species were identified including C. felis (37.5%; 3/8), C. hominis (37.5%; 3/8) and C. parvum (25.0%; 2/8). Three C. hominis subtypes (IbA9G3, IdA14 and IfA12G1) and two C. parvum subtypes (IIdA14G1 and IIdA15G1) were also found.Conclusions: This study was the first to identify the presence of cryptosporidium in asymptomatic children in Southern Xinjiang, China. The presence of zoonotic C. parvum subtypes IIdA14G1 and IIdA15G1 indicates possible crossspecies transmission of Cryptosporidium between children and animals.

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The 16S and COI Mitochondrial DNA Nucleotide Composition of Stripped Snakehead (Channa striata Bloch,1793) from Lake Sentani, Jayapura, Papua

Jurnal Perikanan Universitas Gadjah Mada ◽

10.22146/jfs.35551 ◽

2019 ◽

Vol 20 (2) ◽

pp. 57

Author(s):

Christine Bawaeda Sitandung Kombong ◽

Tuty Arisuryanti

Keyword(s):

Mitochondrial Dna ◽

Fish Species ◽

Nutritional Value ◽

Genetic Characterization ◽

Mitochondrial Gene ◽

Nucleotide Composition ◽

Omega 3 ◽

Channa Striata ◽

Pcr Method

Stripped snakehead (Channa striata Bloch,1793) has potency to be developed in Indonesia due to high nutritional value including omega 3 which is useful for children growth. However, study on composition of mitochondrial DNA nucleotide as a part of study on genetic characterization of stripped snakehead fish in Indonesia is poorlyunderstood. Genetic characterization of stripped snakehead is important to obtain data information that can be applied for breeding and conservation program. Therefore, the objective of this study was to determine the 16S and COI mitochondrial DNA nucleotide of stripped snakehead collected from Lake Sentani, Jayapura, Papua. Method used in this research is PCR method using primer 16Sar and 16Sbr for 16S mitochondrial gene, and FishF2 and FishR2 for COI mitochondrial gene. The results showed that composition of 16S mtDNA nucleotide from 616 bp was T=21.92%, C=25.49%, A=30.19%, dan G=22.40%, whereas composition of A+T=52.11% and G+C=47.89%. In addition, composition of COI mtDNA nucleotide from 705 bp was T=29.93%, C=28.65%, A=24.26%, and G=17.16% while composition of A+T=54.48% and G+C=45.52%. Data of 16S and COI mitochondrial DNA nucleotide composition of the stripped snakehead collected from Lake Sentani gained in this study are to be expected to complete genetic information of the fish species which is useful to improve breeding program of this fish species in the future.

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