scholarly journals The 16S and COI Mitochondrial DNA Nucleotide Composition of Stripped Snakehead (Channa striata Bloch,1793) from Lake Sentani, Jayapura, Papua

2019 ◽  
Vol 20 (2) ◽  
pp. 57
Author(s):  
Christine Bawaeda Sitandung Kombong ◽  
Tuty Arisuryanti
Keyword(s):  
Mitochondrial Dna ◽  
Fish Species ◽  
Nutritional Value ◽  
Genetic Characterization ◽  
Mitochondrial Gene ◽  
Nucleotide Composition ◽  
Omega 3 ◽  
Channa Striata ◽  
Pcr Method

Stripped snakehead (Channa striata Bloch,1793) has potency to be developed in Indonesia due to high nutritional value including omega 3 which is useful for children growth. However, study on composition of mitochondrial DNA nucleotide as a part of study on genetic characterization of stripped snakehead fish in Indonesia is poorlyunderstood. Genetic characterization of stripped snakehead is important to obtain data information that can be applied for breeding and conservation program. Therefore, the objective of this study was to determine the 16S and COI mitochondrial DNA nucleotide of stripped snakehead collected from Lake Sentani, Jayapura, Papua. Method used in this research is PCR method using primer 16Sar and 16Sbr for 16S mitochondrial gene, and FishF2 and FishR2 for COI mitochondrial gene. The results showed that composition of 16S mtDNA nucleotide from 616 bp was T=21.92%, C=25.49%, A=30.19%, dan G=22.40%, whereas composition of A+T=52.11% and G+C=47.89%. In addition, composition of COI mtDNA nucleotide from 705 bp was T=29.93%, C=28.65%, A=24.26%, and G=17.16% while composition of A+T=54.48% and G+C=45.52%. Data of 16S and COI mitochondrial DNA nucleotide composition of the stripped snakehead collected from Lake Sentani gained in this study are to be expected to complete genetic information of the fish species which is useful to improve breeding program of this fish species in the future.

scholarly journals Composition of Mitochondrial DNA 16S Nucleotide of Dwarf Snakehead (Channa gachua Hamilton, 1822) from Keji River, Magelang, Central Java

2018 ◽  
Vol 3 (2) ◽  
pp. 57
Author(s):  
Warisatul Ilmi ◽  
Tuty Arisuryanti
Keyword(s):  
Freshwater Fish ◽  
Fish Species ◽  
Genetic Characterization ◽  
Average Rate ◽  
Nucleotide Composition ◽  
Freshwater Biodiversity ◽  
Mt Dna ◽  
Central Java ◽  
Two Samples ◽  
Pcr Method

Indonesia has a high marine and freshwater biodiversity including freshwater fish biodiversity. One of freshwater fish which is commonly consumed by Indonesian people is dwarf snakehead (Channa gachua Hamilton, 1822). However, research on genetic characterization, especially the composition of mtDNA 16S nucleotide of dwarf snakehead has poorly understood. Therefore, the aim of this study was to determine the composition of mtDNA 16S nucleotide of dwarf snakehead as a part of genetic characterization of the fish species taken from Keji River, Magelang, Central Java which has not been previously examined. This study analyzed 16S mt-DNA of two samples of dwarf snakehead from Keji River (KTS-01 and KTS-02). In addition, two sequences of Channa gachua with accession number KU986900, KU238074, and HM117234-HM117238 taken from GenBank were used as a comparison. A method used in this research was a PCR method and primers used in this research were 16Sar and 16Sbr. The results revealed that the average of nucleotide composition T, C, A and G of the fish species was 23.04%, 25.13%, 29.06% and 22.77% respectively whereas the average rate of nucleotide composition A+ T and G+ C was 52.10% and 47.90% respectively. The two dwarf snakehead had similar T and C composition but different in A and G composition. In addition, the G+C content in KTS-01 and KTS-02 had the highest frequency compared to other dwarf snakehead taken from GenBank. From this finding it could be assumed that there is genetic variation between the two dwarf snakehead from Keji River which is important genetic data for breeding program of the fish species in the future.

Genetic characterization of Phytophthora nicotianae by the analysis of polymorphic regions of the mitochondrial DNA

2011 ◽  
Vol 115 (4-5) ◽  
pp. 432-442 ◽  
Author(s):  
Marco Antonio Mammella ◽  
Santa Olga Cacciola ◽  
Frank Martin ◽  
Leonardo Schena
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Characterization ◽  
Phytophthora Nicotianae

Genetic characterization of Vietnamese Yellow cattle using mitochondrial DNA and Y-chromosomal haplotypes and genes associated with economical traits

2018 ◽  
Vol 89 (12) ◽  
pp. 1641-1647
Author(s):  
Thu N. A. Le ◽  
Hai V. Vu ◽  
Yu Okuda ◽  
Huong T. Duong ◽  
Trung B. Nguyen ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Characterization ◽  
Yellow Cattle

Genetic characterization of cystic pulmonary echinococcosis in Sudan as determined by mitochondrial DNA sequences

2021 ◽  
Vol 04 (03) ◽  
Author(s):  
Sara S Abdalla ◽  
Mohamed E Ahmed ◽  
Alsmawal Awad Elimam ◽  
Mawahib H Eldigail ◽  
Imadeldin E Aradaib ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Dna Sequences ◽  
Genetic Characterization ◽  
Pulmonary Echinococcosis

scholarly journals Genetic characterization of fin fish species from the Warri River at Ubeji, Niger Delta, Nigeria

2014 ◽  
Vol 13 (27) ◽  
pp. 2689-2695
Author(s):  
M. C. Asagbra, ◽  
A. S. Adebayo, ◽  
O. A. Ugwumba, ◽  
A. A. A. Ugwumba, ◽  
C. I. Anumudu,
Keyword(s):  
Fish Species ◽  
Niger Delta ◽  
Genetic Characterization

scholarly journals Genetic Characterization of Kazakh Native Sheep Breeds Using Mitochondrial DNA

2018 ◽  
Vol 18 (3) ◽  
pp. 341-348 ◽  
Author(s):  
Ilmira Mukhametzharova ◽  
Yessenbay Islamov ◽  
Saukymbek Shauyenov ◽  
Dulat Ibrayev ◽  
Sabina Atavliyeva ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Characterization ◽  
Sheep Breeds ◽  
Native Sheep

scholarly journals GENETIC CHARACTERIZATION OF LONGTAIL TUNA Thunnus tonggol (BLEEKER, 1851) BASED ON PARTIAL SEQUENCE OF 16S rRNA MITOCHONDRIAL GENE

2018 ◽  
Vol 24 (2) ◽  
pp. 83
Author(s):  
Achmad Zamroni ◽  
Suwarso Suwarso ◽  
Arif Wibowo
Keyword(s):  
16S Rrna ◽  
Nucleotide Diversity ◽  
Genetic Characterization ◽  
Mitochondrial Gene ◽  
Rrna Gene ◽  
Geographic Scale ◽  
Java Sea ◽  
Mitochondrial 16S Rrna Gene ◽  
Longtail Tuna

Although the Longtail tuna (Thunnus tonggol) is an important fish in Indonesia, the population structure has not been investigated. In this study, the genetic differences in geographic scale are analyzed to provide a clear picture of the structure of T. tonggol populations along a transect stretching from Pemangkat (western Kalimantan) to Pekalongan in the Java Sea. We also analyzed SNPs in the mitochondrial 16S rRNA gene of T. tonggol as potential molecular marker for the identification of the origin within species. In total, three polymorphic sites (all represent singleton dimensions) were identified in the sequence analysis of the 570-bp fragment among a total of 97 T. tonggol individuals from Pekalongan and Pemangkat. Based on these polymorphic sites, four haplotypes were identified. The Pemangkat samples had higher amount of haplotype and nucleotide diversity (h = 0.1556 ± 0.0680 and  = 0.000277 ± 0.000432), meanwhile samples Pekalongan showed lower levels of diversity (h = 0.0400 ± 0.0380 and  = 0.000070 ± 0.000209). The study revealed a single, intermixing population of T. tonggol across the sampled location. No significant structuring was observed between other pairwise comparisons, indicating gene flow between geographically adjacent locations.

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