NMR spectroscopy – a modern analytical tool for serum analytics of lipoproteins and metabolites

AbstractNMR spectroscopy is a modern analytical method which is extremely suitable for the analysis of various body fluids. In addition to small metabolite quantification, the method is capable of differentiated lipoprotein subfraction measurements. The technique can be well standardized and allows extensive automation and good sample throughput compared with lipoprotein fractionation via ultracentrifugation. Because all evaluated parameters are determined simultaneously in one measurement, this method is especially suitable for metabolomics approaches in which even subtle changes in metabolite ratios may be relevant. This review gives an overview of the current methods of NMR spectroscopy, analysis strategies, and practical applications in various studies concerning lipid analysis as well as metabolomics. Over the course of the past years, NMR spectroscopy has heavily evolved from a mere research method into a tool that can be expected to play an important role in routine diagnostic testing in the future.

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Chemometrics Analysis Combined with GC-MS and NMR Spectroscopy Analysis of Fatty Acids as a Means of Discriminating Butterfat Adulteration

Journal of Pharmacy and Nutrition Sciences ◽

10.29169/1927-5951.2019.09.02.5 ◽

2019 ◽

Vol 9 (2) ◽

pp. 87-94

Author(s):

Rashidi Othman ◽

Keyword(s):

Fatty Acids ◽

Nmr Spectroscopy ◽

Spectroscopy Analysis

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Characterization of New Inorganic- Organic Hybrid Compounds: (C6H10N2).Cl2 (I), [C6H10N2]2ZnCl4 (II): Crystal Structure, Hirschfield Surface, IR and NMR spectroscopy Analysis.

10.21203/rs.3.rs-803292/v1 ◽

2021 ◽

Author(s):

nejeh hannachi ◽

faouzi hlel

Keyword(s):

Nmr Spectroscopy ◽

Three Dimensional ◽

X Ray Diffraction ◽

Spectroscopy Analysis ◽

Absorption Bands ◽

Monoclinic System ◽

Xrd Pattern ◽

Organic Hybrid ◽

C Nmr

Abstract Two new organic-inorganic hybrid materials, (C6H10N2).Cl2 (I) and [C6H10N2]2ZnCl4 (II), have been synthesized by hydrothermal method and characterized by single-crystal X-ray diffraction and XRD pattern investigations. These two compounds are crystallized in the monoclinic system; C2/c space group. In the both structures, the anionic-cationic entities are interconnected by hydrogen bonding contacts and p-p Interaction forming three-dimensional networks. Intermolecular interactions were investigated by Hirshfeld surfaces and the contacts of the four different chloride atoms in (II) were compared. The vibrational absorption bands were identified by infrared spectroscopy. These compounds were also investigated by solid state 13C NMR spectroscopy.

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Characterization of soil phosphorus in a fire-affected forest Cambisol by chemical extractions and 31P-NMR spectroscopy analysis

The Science of The Total Environment ◽

10.1016/j.scitotenv.2010.03.035 ◽

2010 ◽

Vol 408 (16) ◽

pp. 3342-3348 ◽

Cited By ~ 30

Author(s):

María-Belén Turrion ◽

Francisco Lafuente ◽

María-José Aroca ◽

Olga López ◽

Rafael Mulas ◽

...

Keyword(s):

Nmr Spectroscopy ◽

31P Nmr ◽

Soil Phosphorus ◽

31P Nmr Spectroscopy ◽

Spectroscopy Analysis ◽

Chemical Extractions

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Inositol phosphates from barley low-phytate grain mutants analysed by metal-dye detection HPLC and NMR

Biochemical Journal ◽

10.1042/bj3540473 ◽

2001 ◽

Vol 354 (2) ◽

pp. 473-480 ◽

Cited By ~ 13

Author(s):

Frank HATZACK ◽

Frank HÜBEL ◽

Wei ZHANG ◽

Poul E. HANSEN ◽

Søren K. RASMUSSEN

Keyword(s):

Nmr Spectroscopy ◽

Inositol Phosphates ◽

Degradation Products ◽

Phytase Activity ◽

Grain Development ◽

Spectroscopy Analysis ◽

Phytate Degradation ◽

Parent Variety ◽

Mutant Lines

Inositol phosphates from barley low-phytate grain mutants and their parent variety were analysed by metal-dye detection HPLC and NMR. Compound assignment was carried out by comparison of retention times using a chemical hydrolysate of phytate [Ins(1,2,3,4,5,6)P6] as a reference. Co-inciding retention times indicated the presence of phytate, D/L-Ins(1,2,3,4,5)P5, Ins(1,2,3,4,6)P5, D/L-(1,2,4,5,6)P5, D/L-(1,2,3,4)P4, D/L-Ins(1,2,5,6)P4 and D/L-Ins(1,4,5,6)P4 in PLP1B mutants as well as the parent variety. In grain extracts from mutant lines PLP1A, PLP2A and PLP3A unusual accumulations of D/L-Ins(1,3,4,5)P4 were observed whereas phytate and the above-mentioned inositol phosphates were present in relatively small amounts. Assignment of D/L-Ins(1,3,4,5)P4 was corroborated by precise co-chromatography with a commercial Ins(1,3,4,5)P4 standard and by NMR spectroscopy. Analysis of inositol phosphates during grain development revealed accumulation of phytate and D/L-Ins(1,3,4,5)P4, which suggested the tetrakisphosphate compound to be an intermediate of phytate synthesis. This assumption was strengthened further by phytate degradation assays showing that D/L-Ins(1,3,4,5)P4 did not belong to the spectrum of degradation products generated by endogenous phytase activity. Metabolic scenarios leading to accumulation of D/L-Ins(1,3,4,5)P4 in barley low-phytate mutants are discussed.

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Quantification of Chlorogenic Acid and Hyperoside Directly from Crude Blueberry (Vaccinium angustifolium) Leaf Extract by NMR Spectroscopy Analysis: Single-Laboratory Validation

Journal of AOAC International ◽

10.5740/jaoacint.11-415 ◽

2012 ◽

Vol 95 (5) ◽

pp. 1406-1411 ◽

Cited By ~ 17

Author(s):

Joshua M Hicks ◽

Asim Muhammad ◽

Jonathan Ferrier ◽

Ammar Saleem ◽

Alain Cuerrier ◽

...

Keyword(s):

Nmr Spectroscopy ◽

Chlorogenic Acid ◽

Mesh Size ◽

Array Detector ◽

Spectroscopy Analysis ◽

Vaccinium Angustifolium ◽

Spectroscopy Experiment ◽

Tube Type ◽

Significant Interference ◽

Single Laboratory

Abstract A single-laboratory-validated NMR spectroscopy method was established for determining the quantity of chlorogenic acid and hyperoside from crude extract material of blueberry leaves of the species Vaccinium angustifolium var. laevifolium House. The calibration curve of chlorogenic acid showed a highly linear regression, R = 0.99998. NMR spectroscopy identification and quantification of the constituents directly from the mixture, within the error of HPLC-diode array detector analysis, were determined as 7.53 mM chlorogenic acid (64.0 mg chlorogenic acid/g powdered leaf) and 0.77 mM hyperoside (8.58 mg hyperoside/g powdered leaf). The LOD was calculated to be 0.01 mM and the LOQ 0.01 mM by the 9 min and 13 s NMR spectroscopy experiment utilized. The assay showed no significant interference from different field strengths, extraction mesh size, gravimetric scale precision, NMR spectroscopy tube type, pulse program, amount of starting dry material, or day-to-day operation. The robustness of NMR spectroscopy as a means of definitively monitoring chlorogenic acid and hyperoside content directly from crude extracts was demonstrated by Youden statistical analysis.

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