scholarly journals Can keratin scaffolds be used for creating three-dimensional cell cultures?

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 249-253
Author(s):  
Marta Bochynska-Czyz ◽  
Patrycja Redkiewicz ◽  
Hanna Kozlowska ◽  
Joanna Matalinska ◽  
Marek Konop ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Chemical Activation ◽  
Three Dimensional ◽  
Enzymatic Digestion ◽  
Pepsin Digestion ◽  
Cell Culturing ◽  
3D Cell Cultures ◽  
Associated Proteins ◽  
Positive Effect ◽  
Dimensional Cell

AbstractThree-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.

scholarly journals Three-Dimensional Cell Cultures in Drug Discovery and Development

2017 ◽  
Vol 22 (5) ◽  
pp. 456-472 ◽  
Author(s):  
Ye Fang ◽  
Richard M. Eglen
Keyword(s):  
Drug Discovery ◽  
Cell Cultures ◽  
Disease Modeling ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Compound Identification ◽  
3D Cell Cultures ◽  
Dimensional Cell ◽  
Early Drug

The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures as systems that better mimic in vivo physiology. Today, 3D cell cultures are emerging, not only as a new tool in early drug discovery but also as potential therapeutics to treat disease. In this review, we assess leading 3D cell culture technologies and their impact on drug discovery, including spheroids, organoids, scaffolds, hydrogels, organs-on-chips, and 3D bioprinting. We also discuss the implementation of these technologies in compound identification, screening, and development, ranging from disease modeling to assessment of efficacy and safety profiles.

Advanced in vitro management of three-dimensional cell cultures and explanted tissue

Cytotherapy ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. S145
Author(s):  
S. Kress ◽  
D. Egger ◽  
C. Kasper
Keyword(s):  
Cell Cultures ◽  
Three Dimensional ◽  
Dimensional Cell

scholarly journals Dispersible oxygen microsensors map oxygen gradients in three-dimensional cell cultures

2017 ◽  
Vol 5 (10) ◽  
pp. 2106-2113 ◽  
Author(s):  
Sasha Cai Lesher-Pérez ◽  
Ge-Ah Kim ◽  
Chuan-hsien Kuo ◽  
Brendan M. Leung ◽  
Sanda Mong ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Three Dimensional ◽  
Oxygen Gradients ◽  
Oxygen Measurements ◽  
Dimensional Cell

Oxygen measurements in different microtissue culture environments were accomplished with the use of phase fluorimetry on dispersible oxygen microsensors.

Glutathione content of V79 cells in two- or three-dimensional culture

1997 ◽  
Vol 272 (5) ◽  
pp. C1507-C1512 ◽  
Author(s):  
F. J. Romero ◽  
D. Zukowski ◽  
W. Mueller-Klieser
Keyword(s):  
Cell Cultures ◽  
Size Range ◽  
Acute Hypoxia ◽  
Three Dimensional ◽  
Central Area ◽  
Cell Number ◽  
Lung Cells ◽  
V79 Cells ◽  
Monolayer Cultures ◽  
Dimensional Cell

The cellular glutathione (GSH) content of two- and three-dimensional cell cultures of V79 hamster lung cells has been studied. As previously described, cells in monolayer cultures show a decrease in GSH when they reach the confluent state. Three-dimensional cell cultures (multicell spheroids) allow a smoother transition from the initial proliferating to the nonproliferating status, and they show a central area of necrosis when a certain diameter is reached. Cellular GSH content in spheroids is variable throughout the culturing period: 1) GSH content (expressed per mg protein) is lower in spheroids with central necrotic areas than in smaller spheroids without necrosis, and 2) results expressed per cell number show a sharp increase around the diameter where necrosis appears. Once a relatively large necrotic area has been established, GSH decreases again to approximately the prenecrotic level. Interestingly, this GSH "peak" is not dependent on the time in culture but on the spheroid size. Acute hypoxia occurs in central areas of spheroids at a much higher size range than those described herein. Thus we suggest a combination of factors, which may include oxidative stress among others, as the explanation for these cellular GSH variations.

An in vitro pulp chamber with three-dimensional cell cultures

1999 ◽  
Vol 25 (1) ◽  
pp. 24-29 ◽  
Author(s):  
Gottfried Schmalz ◽  
Uta Schuster ◽  
Katja Nuetzel ◽  
Helmut Schweikl
Keyword(s):  
Cell Cultures ◽  
Three Dimensional ◽  
Pulp Chamber ◽  
Dimensional Cell

scholarly journals Correction: Scaffold-free three-dimensional cell culturing using magnetic levitation

2018 ◽  
Vol 6 (7) ◽  
pp. 1996-1996
Author(s):  
Esra Türker ◽  
Nida Demirçak ◽  
Ahu Arslan-Yildiz
Keyword(s):  
Magnetic Levitation ◽  
Three Dimensional ◽  
Cell Culturing ◽  
Dimensional Cell

Correction for ‘Scaffold-free three-dimensional cell culturing using magnetic levitation’ by Esra Türker et al., Biomater. Sci., 2018, DOI: 10.1039/c8bm00122g.

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