Standardized technique of water permeability measurement for biomedical applications

Author(s):  
Swen Grossmann ◽  
Stefan Siewert ◽  
Stefanie Kohse ◽  
Robert Ott ◽  
Wolfram Schmidt ◽  
...  
2006 ◽  
Vol 10 (9) ◽  
pp. 1177-1186
Author(s):  
Céline Perlot ◽  
Gérard Ballivy ◽  
Myriam Carcassès ◽  
Xavier Bourbon

10.5109/9266 ◽  
2006 ◽  
Vol 51 (2) ◽  
pp. 431-435
Author(s):  
Aimin Hao ◽  
Atsushi Marui ◽  
Tomokazu Haraguchi ◽  
Yoshisuke Nakano

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Anna Bergstrand ◽  
Sanna Uppström ◽  
Anette Larsson

Poly(3-hydroxybutyrate) (PHB) is a polyester which shows excellent biocompatibility and a PHB material is therefore considered suitable for many biomedical applications. A highly porous PHB material may be designed to facilitate the transport of small molecules and body fluids or serve as a biocompatible temporary barrier. In this study, PHB films with varying degree of porosity and pore interconnectivity were made by solvent casting using water-in-oil emulsion templates of varying composition. The morphology was characterized by SEM and the water permeability of the films was determined. The results show that an increased water content of the template emulsion resulted in a film with increased porosity. A fine tuning of the film morphology of the casted films was achieved by varying the salt content of the water phase of the template emulsion. The porosity of these films was roughly the same but the water permeability varied between23·10-13and1486·10-13 m2/s. It was concluded that the major determinant of the water permeability through these films is the pore interconnectivity. Furthermore, we report on the formation and water permeability of bilayer PHB films consisting of a porous layer combined with a dense backing layer.


Author(s):  
T. L. Hayes

Biomedical applications of the scanning electron microscope (SEM) have increased in number quite rapidly over the last several years. Studies have been made of cells, whole mount tissue, sectioned tissue, particles, human chromosomes, microorganisms, dental enamel and skeletal material. Many of the advantages of using this instrument for such investigations come from its ability to produce images that are high in information content. Information about the chemical make-up of the specimen, its electrical properties and its three dimensional architecture all may be represented in such images. Since the biological system is distinctive in its chemistry and often spatially scaled to the resolving power of the SEM, these images are particularly useful in biomedical research.In any form of microscopy there are two parameters that together determine the usefulness of the image. One parameter is the size of the volume being studied or resolving power of the instrument and the other is the amount of information about this volume that is displayed in the image. Both parameters are important in describing the performance of a microscope. The light microscope image, for example, is rich in information content (chemical, spatial, living specimen, etc.) but is very limited in resolving power.


Author(s):  
Philippe Fragu

The identification, localization and quantification of intracellular chemical elements is an area of scientific endeavour which has not ceased to develop over the past 30 years. Secondary Ion Mass Spectrometry (SIMS) microscopy is widely used for elemental localization problems in geochemistry, metallurgy and electronics. Although the first commercial instruments were available in 1968, biological applications have been gradual as investigators have systematically examined the potential source of artefacts inherent in the method and sought to develop strategies for the analysis of soft biological material with a lateral resolution equivalent to that of the light microscope. In 1992, the prospects offered by this technique are even more encouraging as prototypes of new ion probes appear capable of achieving the ultimate goal, namely the quantitative analysis of micron and submicron regions. The purpose of this review is to underline the requirements for biomedical applications of SIMS microscopy.Sample preparation methodology should preserve both the structural and the chemical integrity of the tissue.


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