Stereoscopic Examination of the Fine Structure of Some Metal Coatings Used for Scanning Electron Microscopy / Stereoskopische Gefügeuntersuchung von in der Rasterelektronenmikroskopie verwendeten Metallbeschichtungen

1983 ◽  
Vol 20 (1) ◽  
pp. 31-41
Author(s):  
Colin Beaton ◽  
Barry Filshie
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
Metal Coatings ◽  
Scanning Electron

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Fibroblasts During Hypertrophic Scar Formation and Resolution

1973 ◽  
Vol 31 ◽  
pp. 428-429
Author(s):  
C. W. Kischer
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Proliferation ◽  
Fine Structure ◽  
Metabolic Activity ◽  
Hypertrophic Scar ◽  
Normal Skin ◽  
Ground Substance ◽  
Hypertrophic Scars ◽  
Scanning Electron

The morphology of the fibroblasts changes markedly as the healing period from burn wounds progresses, through development of the hypertrophic scar, to resolution of the scar by a self-limiting process of maturation or therapeutic resolution. In addition, hypertrophic scars contain an increased cell proliferation largely made up of fibroblasts. This tremendous population of fibroblasts seems congruous with the abundance of collagen and ground substance. The fine structure of these cells should reflect some aspects of the metabolic activity necessary for production of the scar, and might presage the stage of maturation.A comparison of the fine structure of the fibroblasts from normal skin, different scar types, and granulation tissue has been made by transmission (TEM) and scanning electron microscopy (SEM).

Ultrastructure of the soil fungus, Geomyces pannorus

1984 ◽  
Vol 42 ◽  
pp. 738-739
Author(s):  
J. A. Traquair ◽  
E. G. Kokko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
Low Temperatures ◽  
Soil Fungus ◽  
Organic Soils ◽  
Anatomical Studies ◽  
Transmission Electron ◽  
Electron Microscopical ◽  
Scanning Electron

With the advent of improved dehydration techniques, scanning electron microscopy has become routine in anatomical studies of fungi. Fine structure of hyphae and spore surfaces has been illustrated for many hyphomycetes, and yet, the ultrastructure of the ubiquitous soil fungus, Geomyces pannorus (Link) Sigler & Carmichael has been neglected. This presentation shows that scanning and transmission electron microscopical data must be correlated in resolving septal structure and conidial release in G. pannorus.Although it is reported to be cellulolytic but not keratinolytic, G. pannorus is found on human skin, animals, birds, mushrooms, dung, roots, and frozen meat in addition to various organic soils. In fact, it readily adapts to growth at low temperatures.

scholarly journals Improving the Resolution of Sputter-coated Films

2000 ◽  
Vol 8 (2) ◽  
pp. 16-17
Author(s):  
Mary Mager
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
High Resolution ◽  
Carbon Film ◽  
Metal Films ◽  
Pure Gold ◽  
High Magnification ◽  
Conducting Film ◽  
Scanning Electron

After an inquiry from the Microscopy Listserver, I went back to my 1980 copy of Scanning Electron Microscopy, volume I. Several authors had investigated the structure of thin metal films by depositing the films onto carbon-film-covered TEM grids and imaging the films at high magnification. There were several proposals for new devices that have since become standards for high-resolution coaters, but the Listserver inquiry was for a fine conducting film suitabie for high-resolution SEM from an existing sputter coater.There were several factors studied that influenced the fine structure of the films. The first was the materials sputtered: for a given set of conditions of voltage, current and time, platinum gave the finest film, 60% gold-40% palladium (Au/Pd) the next finest and pure gold the least fine.

Fine structure in cholesteric fingerprint texture observed by scanning electron microscopy

2000 ◽  
Vol 44 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Yusong Wu ◽  
Qing Yuan ◽  
Yanming Dong ◽  
Mian Wang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
Scanning Electron

Observations on the Fine Structure of Siliceous Microfossils from the Oamaru Diatomite (Upper Eocene), II. Archaeomonads.

1975 ◽  
Vol 33 ◽  
pp. 76-77
Author(s):  
John P. Jendrzejewski
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Life History ◽  
Fine Structure ◽  
Light Microscopy ◽  
Outer Layer ◽  
Upper Eocene ◽  
Resting Stages ◽  
History Of ◽  
Scanning Electron

Archaeomonads are a fossil group of marine siliceous cysts and are thought to represent resting stages in the life history of chrysophycean algae. The morphology of the cysts is generally simple with spherical, spheroidal, and elliptical outlines common. The cysts are characterized by the presence of an opening (pore) piercing the wall. A thickened structure (neck) often develops around the pore. The cyst usually is composed of one layer of silica although cysts with double and triple layers are common. The outer layer of the cyst may be smooth, or ornamented with spines, warts, pits, flanges or ridges of silica. Due to their small size (3-25 microns in diameter) and to the presence of minute ornamentation, identication of many forms solely with light microscopy is difficult. The application of scanning electron microscopy (SEM) together with light microscopy (LM) enables one to precisely determine the ornamentation and accurately define a species.

Scanning Electron Microscopy of mouse BALB/C/Cd polycystic renal disease

1987 ◽  
Vol 45 ◽  
pp. 932-933
Author(s):  
E. Keyhani ◽  
W. Penasse
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
Renal Disease ◽  
Solid Tumor ◽  
Single Layer ◽  
The Other ◽  
Distal Tubules ◽  
Number Of Cells ◽  
Scanning Electron

Mouse BALB/C/Cd exhibited spontaneous transplantable kidney adenocarcinoma which affected both sexes and involved both kidneys. This disease is characterized by plain cysts and cysts with single or multiple polyps to solid tumors at various stages of evolution. The fine structure of solid tumor was previously described.Single or multiple (up to 10) cysts were present throughout the cortex parenchyma. Their sizes ranged from 1 to 8 mm in diameter. The cysts occurred from both proximal and distal tubules and were composed of a single layer of cubic-cylindrical epithelial cells. Most cysts exhibited single or multiple focal areas of epithelial polypoid hyperplasia. Single polyps consisted of accumulations of hyperplasic cells piling up on top of each other (Fig. l). The other form consisted of single or multiple branched polyps with a central vascularized core (Fig. 2). The number of cells forming polyps hyperplasia varied between a dozen to up to several hundreds of cells.

SEM observations on egg-shell of the Japanese tusser, Antheraea yamamai and Chinese tusser,Antheraea pernyi

1990 ◽  
Vol 48 (3) ◽  
pp. 98-99
Author(s):  
Hu Cui ◽  
Jian Hong ◽  
Gao Oikang ◽  
Wu Xiaojiang ◽  
Ye Gongyin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
Surface Structure ◽  
Shell Surface ◽  
Antheraea Pernyi ◽  
Egg Shells ◽  
Egg Shell ◽  
Scanning Electron

The egg-shell surface structure of the Japanese tusser and Chinese tusser was observed by means of scanning electron microscopy. There were a lot of similarities between the two egg-shells, but the fine structure may be easily distinguished. As to the Japanese tusser, the petals of the petaloid pattern around micropyle were elongate and raised in the middle (Fig. 1); micropylar tubes numbered 11-13 (Figs. 2 and 3); the wider and thicker bank formed irregularly shaped net-like structure of the egg-shell surface other than in the vicinity of micropyle (Fig. 5); and the thickness of the egg-shell was about 70 μm. In the Chinese tusser the petals were shorter, wider,and even; micropylar tubes numbered 8-9 (Fig. 4); the narrow and low bank formed hexagonal, pentagonal, heptagonal, or octagonal net-like structure; the aeropyle wall was well developed, almost the same in size (Figs. 8 and 9); and the thickness of the egg-shell was about 40 μm.

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