scholarly journals An Evaluation of Fertility in Arkansas Primocane-fruiting Blackberries

HortScience ◽  
2010 ◽  
Vol 45 (7) ◽  
pp. 1000-1005 ◽  
Author(s):  
Alisha L. Ruple ◽  
John R. Clark ◽  
M. Elena Garcia
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Fruit Set ◽  
Pollen Viability ◽  
Tube Growth ◽  
Stigma Receptivity ◽  
Cross Pollination ◽  
Self Pollination ◽  
Selection Of

Floral fertility of five primocane-fruiting (PF) blackberry (Rubus L. subgenus Rubus Watson) genotypes (‘Prime-Jim’®, APF-31, ‘Prime-Ark® 45’, APF-59, and APF-77) and three floricane-fruiting (FF) genotypes (‘Navaho’, ‘Natchez’, and ‘Ouachita’) were tested under field conditions using floricane flowers with four pollination treatments: undisturbed open-pollinated, emasculated self-pollinated, emasculated and cross-pollinated with pollen from a similar fruiting type (PF × PF or FF × FF), and emasculated and cross-pollinated with pollen from a different fruiting type (PF × FF or FF × PF). During primocane flowering, three pollination treatments (undisturbed open-pollinated, emasculated selfed, and emasculated cross-pollinated) were used to further test the fertility of the five PF genotypes. Significant differences between cross-pollination treatments and self-pollination were seldom noted with more differences seen in ‘Prime-Jim’® than any other genotype. Cross-pollinating primocane flowers on ‘Prime-Jim’® resulted in significantly higher fruit set, drupelet set, and average berry weight compared with self-pollination. Fruit set among genotypes ranged from 68.5 to 96.7%, and drupelet set rating ranged from 4.3 to 6.9 for floricane flowers. For open-pollinated primocane flowers, fruit set ranged from 63.9 to 92.1%, and drupelet set rating ranged from 4.3 to 7.2. The genotypes APF-31, APF-59, and APF-77 showed a marked improvement over ‘Prime-Jim’® in both percent fruit set and drupelet set of floricane and primocane flowers. The results indicated that fertility appears to be sufficient in all the genotypes evaluated and that the later-generation PF genotypes show improvement in fertility over ‘Prime-Jim’®. Pollen viability (using both chemical viability testing and in vitro germination testing), stigma receptivity, and pollen tube growth (using florescence) were evaluated in a controlled environment to determine if any improvements could be noticed when comparing a selection of later-generation PF genotypes (APF-31,' Prime-Ark® 45', APF-59, and APF-77) with ‘Prime-Jim’® and ‘Prime-Jan’®. Genotypes APF-31, APF-59, and APF-77 had significantly more viable pollen and pollen germination than ‘Prime-Jan’®. Stigma receptivity was observed in all genotypes. Pollen tube growth did not appear to be inhibited after self-pollination in any genotype studied, although significantly greater florescence resulting from pollen tube growth was seen after cross-pollination compared with self-pollination for APF-59. There were no significant differences in pollen tube growth between cross- and self-pollination for any other genotype.

Influences of Cross Pollination on Pollen Tube Growth and Fruit Set in Zuili Plums (Prunus salicina)

2008 ◽  
Vol 50 (2) ◽  
pp. 203-209 ◽  
Author(s):  
Hui-Juan Jia ◽  
Feng-Jie He ◽  
Cai-Zhen Xiong ◽  
Fu-Rong Zhu ◽  
Goro Okamoto
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Fruit Set ◽  
Tube Growth ◽  
Cross Pollination ◽  
Prunus Salicina

scholarly journals Pollen Viability and Vigor in Hybrid Southern Highbush Blueberries (Vaccinium corymbosum L. ×spp.)

HortScience ◽  
1992 ◽  
Vol 27 (5) ◽  
pp. 425-427 ◽  
Author(s):  
Gregory A. Lang ◽  
E. James Parrie
Keyword(s):  
Pollen Tube ◽  
Growth Rates ◽  
Pollen Tube Growth ◽  
Pollen Viability ◽  
Pollen Tubes ◽  
Vaccinium Corymbosum ◽  
Tube Growth ◽  
Multiple Pollen ◽  
Southern Highbush

Pollen from six southern highbush blueberry cultivars derived from Vaccinium corymbosum L. and one or more other species (V. darrowi Camp, V. ashei Reade, and V. angustifolium Aiton) was incubated on nutrient agar to determine tetrad viability, pollen tube growth rates, and incidence of multiple pollen tube germinations. `Avonblue' pollen had a significantly lower tetrad germination percentage than `Georgiagem', `Flordablue', `Sharpblue', `Gulfcoast', or `O'Neal', all of which had >90% viable tetrads. The in vitro growth rate of `O'Neal' pollen tubes was significantly higher than the growth rates of `Sharpblue' and `Georgiagem pollen tubes. Of those tetrads that were viable, more than two pollen tubes germinated from 83% and 91% of the `Gulfcoast' and `Sharpblue' tetrads, respectively, while only 11% of the `Flordablue' tetrads produced more than two pollen tubes. The total number of pollen tubes germinated per 100 tetrads ranged from 157 (`Flordablue') to 324 (`Sharpblue'), resulting in actual pollen grain viabilities ranging from 39% to 81%. Genetic differences in pollen vigor, as indicated by pollen viability, pollen tube growth rates, and multiple pollen tube germinations, may influence blueberry growers' success in optimizing the beneficial effects of cross-pollination on fruit development.

scholarly journals Transcriptomic Analysis Reveals a Comprehensive Calcium- and Phytohormone-Dominated Signaling Response in Leymus chinensis Self-Incompatibility

2019 ◽  
Vol 20 (9) ◽  
pp. 2356 ◽  
Author(s):  
Shuangyan Chen ◽  
Junting Jia ◽  
Liqin Cheng ◽  
Pincang Zhao ◽  
Dongmei Qi ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Time Course ◽  
Molecular Mechanisms ◽  
Tube Growth ◽  
Leymus Chinensis ◽  
Self Incompatibility ◽  
Cross Pollination ◽  
Grass Family ◽  
Self Pollination

Sheepgrass (Leymus chinensis (Trin.) Tzvel.) is an economically and ecologically important forage in the grass family. Self-incompatibility (SI) limits its seed production due to the low seed-setting rate after self-pollination. However, investigations into the molecular mechanisms of sheepgrass SI are lacking. Therefore, microscopic observation of pollen germination and pollen tube growth, as well as transcriptomic analyses of pistils after self- and cross-pollination, were performed. The results indicated that pollen tube growth was rapidly inhibited from 10 to 30 min after self-pollination and subsequently stopped but preceded normally after cross-pollination. Time course comparative transcriptomics revealed different transcriptome dynamics between self- and cross-pollination. A pool of SI-related signaling genes and pathways was generated, including genes related to calcium (Ca2+) signaling, protein phosphorylation, plant hormone, reactive oxygen species (ROS), nitric oxide (NO), cytoskeleton, and programmed cell death (PCD). A putative SI response molecular model in sheepgrass was presented. The model shows that SI may trigger a comprehensive calcium- and phytohormone-dominated signaling cascade and activate PCD, which may explain the rapid inhibition of self-pollen tube growth as observed by cytological analyses. These results provided new insight into the molecular mechanisms of sheepgrass (grass family) SI.

scholarly journals Compatibility Relationships in `Manzanillo' Olive

HortScience ◽  
1997 ◽  
Vol 32 (6) ◽  
pp. 1056-1058 ◽  
Author(s):  
Julián Cuevas ◽  
Vito S. Polito
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Olea Europaea ◽  
Fruit Set ◽  
Fold Increase ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Central California ◽  
Self Pollination ◽  
Olea Europaéa

We investigated pollination and fruit set parameters in `Manzanillo' olive (Olea europaea L.) following self-pollination and pollination with `Sevillano', `Ascolano', and `Mission' pollen. Results of analyses and experiments conducted over 2 years in central California indicated that `Manzanillo' behaves as a self-incompatible cultivar (index of self-incompatibility = 0.22 to 0.24). Pollination with `Sevillano' resulted in a more than 4-fold increase in fruit set over self-pollination. When `Mission' or `Ascolano' pollen was used, there was no increase over self-pollinated samples. Analyses of pollen tube growth, fertilization, initial fruit set, and final fruit set were consistent with `Manzanillo' being considered as a self-incompatible cultivar cross-incompatible with `Mission' and `Ascolano'. Our results indicate that `Manzanillo' is likely to be more productive when interplanted with `Sevillano' rather than when planted without a pollinizer or with `Mission' or `Ascolano'.

scholarly journals Novel Metrics to Characterize In Vitro Pollen Tube Growth Performance of Apple Cultivars

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1460
Author(s):  
Stefan Roeder ◽  
Sara Serra ◽  
Stefano Musacchi
Keyword(s):  
Decision Tree ◽  
Pollen Tube ◽  
Growth Performance ◽  
Pollen Tube Growth ◽  
Pollen Viability ◽  
Tube Growth ◽  
Tree Model ◽  
Apple Cultivars ◽  
Screening Trials

In vitro germination assays are frequently used in screening trials to evaluate the pollen viability of pollinizers. To be effective, screening trials must have defined threshold criteria, from which individuals can then be assessed. However, despite decades of research on pollen viability, no established threshold is available to categorize apple cultivars based on their in vitro pollen tube lengths. This study aimed to identify and characterize the subgroups of cultivars based on their pollen tube growth performance. In vitro pollen tube lengths of 41 individuals were determined by incubating samples on artificial germination media at 15 and 25 °C. A six-number summary statistic was calculated, and hierarchical clustering on principal component (HCPC) analysis was used to determine and characterize subgroups. Furthermore, a decision tree model was used to predict class membership for future datasets. HCPC analysis partitioned the 41 individuals into three subgroups with different performances. The decision tree quickly predicted the cluster membership based on the second quartile at 15 °C and the third quartile at 25 °C. The thresholds from the decision tree can be used to characterize new observations. The use of the methods will be demonstrated using a case study with 29 apple accessions.

scholarly journals Reciprocal intercompatibility between Byrsonima Rich. exKunth species (Malpighiaceae), in an urban ecotonal fragment of Semi-Deciduous Seasonal Forest and Cerrado

2021 ◽  
pp. 211-221
Author(s):  
Frederic Mendes Hughes
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Prezygotic Isolation ◽  
Stigmatic Surface ◽  
Cross Pollination ◽  
Self Pollination ◽  
Isolation Mechanisms ◽  
Hypothesis Evaluation

Byrsonima species present floral monomorphism and sharing visitor/pollinator guild. These traits suggest the hypothesis evaluation of reciprocal inter-incompatibility between two syntopic Byrsonima species: B. pachyphylla and B. crassifolia. Pollen tube growth with similar behavior was observed in the stigmatic surface, pistil canal, ovary and micropylar channel to both species. In addition, partial self-incompatibility in self-pollination with greater fruiting in autogamy was observed. Cross-pollination and self-pollination coexist, and reciprocal intercompatibility occurs. Prezygotic isolation mechanisms are unlikely by the absence of abnormal pollen tubes, higher fruiting production and absence of hybrids in the study site. Las especies de Byrsonima presentan monomorfismo floral y comparten gremio de visitantes/polinizadores. Estos rasgos sugieren la evaluación de hipótesis de interincompatibilidad recíproca entre dos especies sintópicas de Byrsonima: B. pachyphylla y B. crassifolia. Se observó el crecimiento del tubo polínico con comportamiento similar en la superficie estigmática, canal del pistilo, ovario y canal micropilar de ambas especies. Además, se describió la autoincompatibilidad parcial en la autopolinización con mayor fructificación en la autogamia. La polinización cruzada y la autopolinización coexisten y se produce una intercompatibilidad recíproca. Los mecanismos de aislamiento precigóticos son improbables por la ausencia de tubos polínicos anormales, producción de frutos y ausencia de híbridos en la área de estudio.

scholarly journals Effective Pollination Period in Almond Cultivars

HortScience ◽  
2004 ◽  
Vol 39 (1) ◽  
pp. 19-22 ◽  
Author(s):  
E. Ortega ◽  
J. Egea ◽  
F. Dicenta
Keyword(s):  
High Temperature ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Fruit Set ◽  
Fruit Trees ◽  
Prunus Dulcis ◽  
Tube Growth ◽  
Stigma Receptivity ◽  
Flower Density

In almond [Prunus dulcis (Mill.) D.A. Webb], a high flower density and fruit set rate is important, because yield increases with higher fruit set ratio. Furthermore, because the ovule of some cultivars mature at anthesis, rapid pollination and pollen tube growth along the style are essential to ensure fertilization of a viable ovule. In this work, we studied the effective pollination period (EPP) of four almond cultivars of different bloom time by studying pollen tube growth and fruit set. EPP in almond was longer than in other fruit trees, and its duration was determined by stigma receptivity, which decreased with high temperature. An acceptable fruit set for all cultivars was obtained following pollination from day 0 to day 4 after emasculation.

scholarly journals Comparison of different classical and molecular methods for identifying self-incompatibility in two olive cultivars

2019 ◽  
Vol 17 (3) ◽  
pp. e0804 ◽  
Author(s):  
Elham Aslmoshtaghi ◽  
Ali Reza Shahsavar ◽  
Majid Talebi ◽  
Aziz Dazeh
Keyword(s):  
Pollen Tube ◽  
Molecular Analysis ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Research Station ◽  
Self Incompatibility ◽  
Controlled Pollination ◽  
Cross Pollination ◽  
Self Pollination ◽  
Olive Cultivars

Aim of study: To determine compatibility relationships and select suitable pollinizers for two olive (Olea europaea L.) cultivars (‘Amygdalolia’ and ‘Konservalia’).Area of study: The Kazeroun Olive Research Station, Fars, Iran.Material and methods: Emasculated flowers from ‘Amygdalolia’ and ‘Konservalia’ cultivars were treated with self-pollination, open-pollination, and cross-pollination with pollen from cultivars such as ‘Dacal’, ‘Amygdalolia’,‘Konservalia’, ‘Koroniki’, and ‘Manzanilla’. Controlled pollination, pollen tube growth, and molecular analysis were employed.Main results: Controlled pollination, pollen tube growth, and molecular analysis showed that cross-pollination was beneficial for ‘Amygdalolia’ compared to self-pollination. The results showed that this cultivar is self-incompatible, and its best pollinator is the ‘Dacal’ cultivar. Experiment results indicated that ‘Konservalia’ behaves as a self-compatible cultivar. The highest fruit percentage and higher pollen tube growth rates were found in self-pollination treatments. Molecular attempts to isolate candidates for sporophytic self-incompatibility (SSI) led the researchers to analyze the expression of SRK and SLG genes.Research highlights: The results indicated an antagonist transcriptional expression pattern in the flowers of ‘Amygdalolia’, classified as a self-incompatible cultivar, and ‘Konservalia’, classified as a self-compatible cultivar, for the SRK and SLG genes.

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