A Thin Layer Chromatographic Technique for the Determination of Residual‚ Oily Soil Components in Detergency Tests

1974 ◽  
Vol 11 (2) ◽  
pp. 98-101
Author(s):  
Marvin C. Allen
Keyword(s):  
Thin Layer ◽  
Chromatographic Technique ◽  
Oily Soil ◽  
Soil Components

II. Biochemical Investigations

1972 ◽  
Vol 18 (5) ◽  
pp. 462-470 ◽  
Author(s):  
C Rimington ◽  
W G Sears ◽  
L Eales
Keyword(s):  
Solvent Extraction ◽  
Thin Layer ◽  
Soluble Fraction ◽  
Characteristic Pattern ◽  
Chromatographic Technique ◽  
Extraction Procedures ◽  
Felty's Syndrome ◽  
Chromatographic Procedure ◽  
Felty’S Syndrome

Abstract The urinary porphyrin excretion of the patient (Part I) has been examined. Methods for determination of the various porphyrin fractions have also been compared and critically assessed. A thin-layer chromatographic technique was found to be rapid, discriminating, and precise. Of solvent-extraction procedures, that of Rimington and Benson for the determination of urinary "X" porphyrins gives results that compare well with those obtained by the quantitative chromatographic procedure. The patient excreted relatively large amounts of uroporphyrin, heptacarboxylic porphyrin, and "X" porphyrins. In addition, the hydroxycoproporphyrins 1 and 2 of Elder were present in the ether-soluble fraction of her urine in relatively large quantities during the period when the patient was severely ill. Her fecal porphyrins were not markedly increased. The porphyria exhibited by this case of Felty’s syndrome resembled the characteristic pattern of symptomatic porphyria, usually associated with alcoholism, but in this case other factors must be considered because this patient was a life-long abstainer from alcohol.

Thin-layer chromatographic determination of urinary excretion of lactulose, simplified and applied to cystic fibrosis patients.

1987 ◽  
Vol 33 (7) ◽  
pp. 1211-1212 ◽  
Author(s):  
J A Flick ◽  
R L Schnaar ◽  
J A Perman
Keyword(s):  
Cystic Fibrosis ◽  
Oral Administration ◽  
Thin Layer ◽  
Urinary Excretion ◽  
Healthy Subjects ◽  
Chromatographic Determination ◽  
Solvent System ◽  
Chromatographic Technique ◽  
High Reproducibility

Abstract Urinary excretion of orally administered lactulose is used as an index of intestinal permeability. We have developed a simple thin-layer chromatographic technique for measuring lactulose in urine, using silica gel 60 plates and a propanol-borate solvent system. Lactulose concentrations as low as 62.5 mg/L can be detected with high reproducibility and without interference by urinary chromogens. After oral administration, the urinary excretion of lactulose in 8 h equaled 2.33 (SD 1.86)% in 15 patients with cystic fibrosis, as compared with 0.13 (SD 0.12)% in 16 healthy subjects (P less than 0.001).

scholarly journals Development of High-performance Thin-layer Chromatography (HPTLC) Validated Method for Simultaneous Quantification of Eucalyptol and α-Pinene in Lamiaceae Plants

2019 ◽  
pp. 1-11 ◽  
Author(s):  
Ahmed Ibrahim Foudah ◽  
Prawez Alam ◽  
Aftab Alam ◽  
Mohammed Ayman Salkini ◽  
Mohammed Hamed Alqarni ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Chromatographic Technique ◽  
Microbial Infections ◽  
Traditional Preparation ◽  
Layer Chromatography ◽  
Hptlc Method ◽  
Ich Guideline

Aim and Objective: Several plants from Lamiaceae family are used in the Saudi Arabia as a condiment and food preparation, and are generally used in the traditional preparation to treat various diseases, including anti-inflammatory, antioxidant, and microbial infections. Some of Lamiaceae species such as Mentha longifolia, Rosemarinus officinalis and Salvia officinalis having pharmacological active compounds such as α-pinene and eucalyptol. The aim of present study was to develop an accurate and precise chromatographic technique for quantification of α-pinene and eucalyptol in the Lamiaceae plants.  Methods: The high-performance thin layer chromatography (HPTLC) method was developed as per International Conference on Harmonization (ICH) guideline. Results: Simultaneous determination of α-pinene and eucalyptol was achieved by developing a densitometric analysis of high-performance thin layer chromatography (HPTLC). Silica gel 60 F254 glass-backed plates (E-Merck, Germany, 0.2 mm layers) as stationary phase and mixture n-hexane: ethyl acetate 8 : 2 (%, v/v) as mobile phase were used to produce a sharp, symmetrical and well-resolved peak at an Rf value of 0.19 ± 0.02 and 0.52 ± 0.04 for α-pinene and eucalyptol, respectively. Linearly range for α-pinene was 100–700 ng/spot (r2 = 0.9988), whereas that for eucalyptol was 1000–7000 ng/spot (r2 = 0.9987). Conclusion: The developed method was found to be a simple, accurate, and precise, and it may be used to simultaneously analyses of many medicinal plants samples containing α-pinene and eucalyptol.

Colorimetric Assay of Strychnine and Brucine in Nux Vomica

1975 ◽  
Vol 58 (1) ◽  
pp. 85-87
Author(s):  
Mohamed S Karawya ◽  
Mohamed S Hifnawy ◽  
Hasan S Dahawi
Keyword(s):  
Nitric Acid ◽  
Thin Layer ◽  
Stannous Chloride ◽  
Colorimetric Assay ◽  
Chromatographic Technique ◽  
Colorimetric Analysis ◽  
Violet Color ◽  
Colorimetric Methods

Abstract Two colorimetric methods are described for the estimation of strychnine and brucine in nux vomica. The first is a modification of the Karawya and Ghourab method for the determination of strychnine, in which the sensitivity of the color is increased by changing certain conditions of the method. The second was developed for the determination of brucine and is based on measuring the intensity of the violet color produced by treating brucine with nitric acid and methanolic stannous chloride. In the presence of large amounts of strychnine, brucine is isolated prior to colorimetric analysis by a quantitative thin layer chromatographic technique.

Development of a method for multielemental determination in water by EDXRF with radioisotopic source of 238Pu.

2019 ◽  
Vol 7 (2A) ◽  
Author(s):  
Camilo Fuentes Serrano ◽  
Juan Reinaldo Estevez Alvares ◽  
Alfredo Montero Alvarez ◽  
Ivan Pupo Gonzales ◽  
Zahily Herrero Fernandez ◽  
...  
Keyword(s):  
Thin Layer ◽  
Expanded Uncertainty ◽  
Considerable Decrease ◽  
X Ray ◽  
Precipitation Efficiency ◽  
Method Of Determination ◽  
Sensitivity Curves ◽  
Order Of Magnitude ◽  
Metrological Parameters

A method for determination of Cr, Fe, Co, Ni, Cu, Zn, Hg and Pb in waters by Energy Dispersive X Ray Fluorescence (EDXRF) was implemented, using a radioisotopic source of 238Pu. For previous concentration was employed a procedure including a coprecipitation step with ammonium pyrrolidinedithiocarbamate (APDC) as quelant agent, the separation of the phases by filtration, the measurement of filter by EDXRF and quantification by a thin layer absolute method. Sensitivity curves for K and L lines were obtained respectively. The sensitivity for most elements was greater by an order of magnitude in the case of measurement with a source of 238Pu instead of 109Cd, which means a considerable decrease in measurement times. The influence of the concentration in the precipitation efficiency was evaluated for each element. In all cases the recoveries are close to 100%, for this reason it can be affirmed that the method of determination of the studied elements is quantitative. Metrological parameters of the method such as trueness, precision, detection limit and uncertainty were calculated. A procedure to calculate the uncertainty of the method was elaborated; the most significant source of uncertainty for the thin layer EDXRF method is associated with the determination of instrumental sensitivities. The error associated with the determination, expressed as expanded uncertainty (in %), varied from 15.4% for low element concentrations (2.5-5 μg/L) to 5.4% for the higher concentration range (20-25 μg/L).

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