Effect of Some Psychotropic Drugs on Luminol - Dependent Chemiluminescence Induced by O2–, •OH, HOCl

We studied antioxidant activity of six neuroleptics (chlorpromazine, levomepromazine, promethazine, trifluoperazine and thioridazine) and two antidepressants (imipramine and amitriptyline) in the range of concentration of 10−7−10−4 м. We applied luminol-dependent chemiluminescence to test the ability of these drugs to scavenge the biologically relevant oxygen-derived species: hydroxyl radical, superoxide radical, hypochlorous acid in vitro. We found that the phenothiazines were powerful scavengers of hydroxyl and superoxide radicals. Chlorprothixene, amitriptyline and imipramine had no scavenge activity to the superoxide radical. All drugs showed a moderate scavenger effect on hypochloric anion.

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Antioxidant Activity of Hypericum androsaemum Infusion: Scavenging Activity against Superoxide Radical, Hydroxyl Radical and Hypochlorous Acid

Biological and Pharmaceutical Bulletin ◽

10.1248/bpb.25.1320 ◽

2002 ◽

Vol 25 (10) ◽

pp. 1320-1323 ◽

Cited By ~ 90

Author(s):

Patrícia Valentão ◽

Eduarda Fernandes ◽

Félix Carvalho ◽

Paula Branquinho Andrade ◽

Rosa Maria Seabra ◽

...

Keyword(s):

Antioxidant Activity ◽

Hydroxyl Radical ◽

Superoxide Radical ◽

Hypochlorous Acid ◽

Scavenging Activity

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Studies on the Antioxidant Activity of Lippia citriodora Infusion: Scavenging Effect on Superoxide Radical, Hydroxyl Radical and Hypochlorous Acid

Biological and Pharmaceutical Bulletin ◽

10.1248/bpb.25.1324 ◽

2002 ◽

Vol 25 (10) ◽

pp. 1324-1327 ◽

Cited By ~ 49

Author(s):

Patrícia Valentão ◽

Eduarda Fernandes ◽

Félix Carvalho ◽

Paula Branquinho Andrade ◽

Rosa Maria Seabra ◽

...

Keyword(s):

Antioxidant Activity ◽

Hydroxyl Radical ◽

Superoxide Radical ◽

Hypochlorous Acid ◽

Lippia Citriodora ◽

Scavenging Effect

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Antioxidant Activities of Flavonoids from Canavalia maritime

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.834-836.577 ◽

2013 ◽

Vol 834-836 ◽

pp. 577-581

Author(s):

Jian Chao Deng ◽

Gai Gai Niu ◽

Lai Hao Li ◽

Xian Qing Yang ◽

Yong Chuan Deng ◽

...

Keyword(s):

Solvent Extraction ◽

Hydroxyl Radical ◽

High Speed ◽

Superoxide Radical ◽

Antioxidant Activities ◽

Reducing Power ◽

Dpph Radical ◽

Inhibitory Effects ◽

Counter Current

Flavonoids was isolated from canavalia maritime by accelerated solvent extraction (ASE) coupled with high-speed counter-current chromatography (HSCCC). The antioxidant activities of flavonoids were investigated in vitro and evaluated by IC50. The results indicated that flavonoids possessed obvious reducing power and significant inhibitory effects on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical. These results suggest that canavalia maritime flavonoids could be a suitable natural antioxidant for humans.

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Evaluation of antioxidant activity of Melothria maderaspatana in vitro

Open Life Sciences ◽

10.2478/s11535-010-0005-5 ◽

2010 ◽

Vol 5 (2) ◽

pp. 224-230 ◽

Cited By ~ 7

Author(s):

Boobalan Raja ◽

Kodukkur Pugalendi

Keyword(s):

Hydrogen Peroxide ◽

Antioxidant Activity ◽

Free Radical ◽

Hydroxyl Radical ◽

Superoxide Anion ◽

Radical Scavenging ◽

Reducing Power ◽

Free Radical Scavenging ◽

Melothria Maderaspatana

AbstractIn this study, an aqueous extract of leaves from Melothria maderaspatana was tested for in vitro antioxidant activity. Free radical scavenging assays, such as hydroxyl radical, hydrogen peroxide, superoxide anion radical and 2,2-diphenyl-1-picryl hydrazyl (DPPH), 2,2’-azinobis-(3-ethyl-enzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and reducing power assay, were studied. The extract effectively scavenged hydroxyl radical, hydrogen peroxide and superoxide anion radicals. It also scavenged DPPH and ABTS radicals. Furthermore, it was found to have reducing power. All concentrations of leaf extract exhibited free radical scavenging and antioxidant power, and the preventive effects were in a dose-dependent manner. The antioxidant activities of the above were compared to standard antioxidants such as butylated hydroxytoluene (BHT), ascorbic acid, and α-tocopherol. The results obtained in the present study indicate that the M. maderaspatana extract could be considered a potential source of natural antioxidant.

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Antioxidant and antifungal activities of Camellia sinensis (L.) Kuntze leaves obtained by different forms of production

Brazilian Journal of Biology ◽

10.1590/1519-6984.18814 ◽

2016 ◽

Vol 76 (2) ◽

pp. 428-434 ◽

Cited By ~ 14

Author(s):

L. E. A. Camargo ◽

L. S. Pedroso ◽

S. C. Vendrame ◽

R. M. Mainardes ◽

N. M. Khalil

Keyword(s):

Antioxidant Activity ◽

Camellia Sinensis ◽

Hypochlorous Acid ◽

Black Tea ◽

Total Phenolic ◽

Myeloperoxidase Activity ◽

Conjugated Diene ◽

Candida Spp ◽

Anticandidal Activity

Abstract The antioxidant and anticandidal activities of leaves obtained from Camellia sinensis by non-fermentation (green and white teas), semi-fermentation (red tea) and fermentation method (black tea) were investigated. It was evaluated the total phenolic content by Folin-Ciocalteau assay; antioxidant capacities were evaluated in vitro using DPPH and ABTS radicals, hypochlorous acid and superoxide anion scavenger assays, induced hemolysis, lipid peroxidation by conjugated diene formation and myeloperoxidase activity. Anticandidal activity was performed on three strains of Candida spp. The results showed that non-fermented teas have a higher concentration of phenolic compounds, and then presented the best inhibitory activity of AAPH-induced hemolysis, the best inhibition of conjugated diene formation and more pronounced antioxidant activity in all tests. The highest anticandidal activity was obtained from fermented tea, followed by non-fermented tea. These results indicate that the antioxidant activity demonstrated has no direct relation with the anticandidal activity.

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Antioxidative properties of tronchuda cabbage (Brassica oleracea L. var. costata DC) external leaves against DPPH, superoxide radical, hydroxyl radical and hypochlorous acid

Food Chemistry ◽

10.1016/j.foodchem.2005.06.019 ◽

2006 ◽

Vol 98 (3) ◽

pp. 416-425 ◽

Cited By ~ 41

Author(s):

Vendula Vrchovská ◽

Carla Sousa ◽

Patrícia Valentão ◽

Federico Ferreres ◽

José A. Pereira ◽

...

Keyword(s):

Hydroxyl Radical ◽

Brassica Oleracea ◽

Superoxide Radical ◽

Hypochlorous Acid ◽

Antioxidative Properties ◽

Tronchuda Cabbage ◽

Brassica Oleracea L

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Studies on antioxidant activity of different extracts of aerial parts of Cadaba farinose Forsk: An in-vitro techniques

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i3.2392 ◽

2020 ◽

Vol 11 (3) ◽

pp. 2986-2992

Author(s):

Jambula Dinesh Babu ◽

Venugopalan Santhosh Kumar

Keyword(s):

Antioxidant Activity ◽

Superoxide Radical ◽

Bioactive Compound ◽

Antioxidant Potential ◽

Total Antioxidant Activity ◽

In Vitro Techniques ◽

Aerial Parts ◽

Total Antioxidant ◽

The Difference

Cadaba farinosa (family Capparidaceae) is generally known as “Indian cadaba” in the traditional ayurvedic system. The current study, aerial parts of different concentrates (Pet.ether, ethyl acetate and methanol) of Cadaba farinose was evaluated for its in-vitro antioxidant potential by Diphenylpicrylhydrazyl radical, and total antioxidant activity taking ascorbate as standard and superoxide radical activity taking Quercetin as the standard for the in-vitro methods. The methanolic concentrates of Cadaba farinose& ascorbic acid exhibited antioxidant potential possessing IC50 208µg/ml & 66µg/ml (Diphenylpicrylhydrazyl radical) , 188µg/ml & 57µg/ml (total antioxidant). The methanolic concentrates of Cadaba farinose & quercetin exhibited antioxidant potential possessing IC50 252µg/ml & 60µg/ml (superoxide radical). The IC50 value was originated that methanolic concentrates of Cadaba farinose more efficient in Diphenylpicrylhydrazyl radical, superoxide radical activity, total antioxidant activity compared EA & PE concentrates. The difference in scavenging potential of the extracts can be due to variation in the percentage of bioactive compound present in different solvents. Invitro antioxidant studies show methanolic concentrates of Cadaba farinose have better antioxidant activity. This result indicates that aerial parts of methanolic concentrate Cadaba farinose could serve as a natural antioxidant, which may be useful in preventing free radical-induced diseases.

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Antioxidant Activity and Mechanism of Protocatechuic Acid in vitro

Functional Foods in Health and Disease ◽

10.31989/ffhd.v1i7.127 ◽

2011 ◽

Vol 1 (7) ◽

pp. 232 ◽

Cited By ~ 100

Author(s):

Xican Li ◽

Xiaozhen Wang ◽

Dongfeng Chen ◽

Shuzhi Chen

Keyword(s):

Antioxidant Activity ◽

Hydroxyl Radical ◽

Superoxide Anion ◽

Protocatechuic Acid ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Chelating Ability ◽

Positive Controls

Background: Protocatechuic acid (PCA) is a natural phenolic acid widely distributed in plants and is considered as an active component of some traditional Chinese herbal medicines such as Cibotium barometz (L.) J.Sm, Stenoloma chusanum (L.) Ching, Ilex chinensis Sims. PCA was reported to possess various pharmacological effects which may be closely correlated with its antioxidant activities. However, the antioxidant of PCA has not been investigated systematically yet. Methods: In the study, the antioxidant activities of protocatechuic acid were measured in vitro using various antioxidant assays including 1,1-diphenyl-2-picryl-hydrazyl (DPPH•), 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS+•), superoxide anion radicals (•O2-) and hydroxyl radical (•OH) scavenging activity, ferric ions (Fe3+) and cupric ions (Cu2+) reducing power, ferrous ions (Fe2+) and cupric ions (Cu2+) chelating activity, compared with the positive controls Trolox or BHT.Results: In all assays, PCA along with positive controls exhibited dose-dependently antioxidant ability. Comparing to a standard antioxidant Trolox, the relative antioxidant activity of PCA (i.e. the ratio of IC50(Trolox)/IC50(PCA) ) was calculated as 2.8, 2.3, 3.7, 6.1, 4.2, 1.0, 2.7, 1.5, respectively, for DPPH, ABTS, reducing power (Fe3+), reducing power (Cu2+), superoxide anion radical-scavenging, hydroxyl radical-scavenging, chelating ability (Fe2+) and chelating ability (Cu2+). Conclusion: Comparing to Trolox, PCA shows much more effective antioxidant activity in vitro in both lipid and aqueous media. Hence, it could therefore be used in pharmacological or food industry as a natural antioxidant. It may exhibit antioxidant activity by both chelating metal transition ions as well as by scavenging free radicals via donating hydrogen atom (H•) or electron (e).Keywords: Protocatechuic acid, antioxidant, reducing power, free radical-scavenging, chelating ability.

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Increased Phenolic Content and Enhanced Antioxidant Activity in Fermented Glutinous Rice Supplemented with Fu Brick Tea

Molecules ◽

10.3390/molecules24040671 ◽

2019 ◽

Vol 24 (4) ◽

pp. 671 ◽

Cited By ~ 7

Author(s):

Xiao Xu ◽

Wenxiu Hu ◽

Siduo Zhou ◽

Chuanhai Tu ◽

Xiudong Xia ◽

...

Keyword(s):

Antioxidant Activity ◽

Hydroxyl Radical ◽

Phenolic Content ◽

Reducing Power ◽

Total Phenolic ◽

Food Ingredient ◽

Antioxidant Power ◽

Functional Activities ◽

Glutinous Rice

Glutinous rice-based foods have a long history are consumed worldwide. They are also in great demand for the pursuit of novel sensory and natural health benefits. In this study, we developed a novel fermented glutinous rice product with the supplementation of Fu brick tea. Using in vitro antioxidant evaluation and phenolic compounds analysis, fermentation with Fu brick tea increased the total phenolic content and enhanced the antioxidant activity of glutinous rice, including scavenging of 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical, 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical, and hydroxyl radical, ferric-reducing antioxidant power, and ferric ion reducing power and iron chelating capability. Besides, compared with traditional fermented glutinous rice, this novel functional food exhibited a stronger activity for protecting DNA against hydroxyl radical-induced oxidation damage. Quantitative analysis by HPLC identified 14 compounds covering catechins and phenolic acids, which were considered to be positively related to the enhanced antioxidant capability. Furthermore, we found that 80% ethanol was a suitable extract solvent compared with water, because of its higher extraction efficiency and stronger functional activities. Our results suggested that this novel fermented glutinous rice could serve as a nutraceutical food/ingredient with special sensory and functional activities.

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