Biosurfactant Production By A New Pseudomonas Putida Strain

2002 ◽  
Vol 57 (3-4) ◽  
pp. 356-360 ◽  
Author(s):  
Borjana K. Tuleva ◽  
George R. Ivanov ◽  
Nelly E. Christova
Keyword(s):  
Surface Tension ◽  
Carbon Source ◽  
Pseudomonas Putida ◽  
Sole Carbon Source ◽  
Biosurfactant Production ◽  
Emulsifying Activity ◽  
Pseudomonas Spp ◽  
Poorly Soluble ◽  
Surface Active

Observation of both tensio-active and emulsifying activities indicated that biosurfactants were produced by the newly isolated and promising strain Pseudomonas putida 21BN. The biosurfactants were identified as rhamnolipids, the amphiphilic surface-active glycolipids usually secreted by Pseudomonas spp. Their production was observed when the strain was grown on soluble substrates, such as glucose or on poorly soluble substrates, such as hexadecane, reaching values of 1.2 g l-1. When grown on hexadecane as the sole carbon source the biosurfactant lowered the surface tension of the medium to 29 mN m-1 and formed stable and compact emulsions with emulsifying activity of 69%

Gordonia (nocardia) amarae foaming due to biosurfactant production

2002 ◽  
Vol 46 (1-2) ◽  
pp. 519-524 ◽  
Author(s):  
K.R. Pagilla ◽  
A. Sood ◽  
H. Kim
Keyword(s):  
Surface Tension ◽  
Carbon Source ◽  
Stationary Phase ◽  
Activated Sludge ◽  
Sole Carbon Source ◽  
Wastewater Treatment Plants ◽  
Carbon Sources ◽  
Biomass Concentration ◽  
Culture Broth ◽  
Biosurfactant Production

Gordonia amarae, a filamentous actinomycete, commonly found in foaming activated sludge wastewater treatment plants was investigated for its biosurfactant production capability. Soluble acetate and sparingly soluble hexadecane were used as carbon sources for G. amarae growth and biosurfactant production in laboratory scale batch reactors. The lowest surface tension (critical micelle concentration, CMC) of the cell-free culture broth was 55 dynes/cm when 1,900 mg/L acetate was used as the sole carbon source. The lowest surface tension was less than 40 dynes/cm when either 1% (v/v) hexadecane or a mixture of 1% (v/v) hexadecane and 0.5% (w/v) acetate was used as the carbon source. The maximum biomass concentration (the stationary phase) was achieved after 4 days when acetate was used along with hexadecane, whereas it took about 8 days to achieve the stationary phase with hexadecane alone. The maximum biosurfactant production was 3 × CMC with hexadecane as the sole carbon source, and it was 5 × CMC with the mixture of hexadecane and acetate. Longer term growth studies (∼ 35 days of culture growth) indicated that G. amarae produces biosurfactant in order to solubilize hexadecane, and that adding acetate improves its biosurfactant production by providing readily degradable substrate for initial biomass growth. This research confirms that the foaming problems in activated sludge containing G. amarae in the activated sludge are due to the biosurfactant production by G. amarae when hydrophobic substrates such as hexadecane are present.

scholarly journals Studies on poly-3-hydroxyoctanoate biosynthesis by a consortium of microorganisms

2016 ◽  
Vol 27 (1) ◽  
pp. 44-47 ◽  
Author(s):  
Mihaela Carmen Eremia ◽  
Irina Lupescu ◽  
Mariana Vladu ◽  
Maria Petrescu ◽  
Gabriela Savoiu ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Carbon Source ◽  
Pseudomonas Putida ◽  
Sole Carbon Source ◽  
Bacterial Biomass ◽  
Fermentation Medium ◽  
Energy Reserve ◽  
Bacterial Strains ◽  
Sodium Octanoate ◽  
Intracellular Energy

Abstract Polyhydroxyalcanoates (PHAs) are specifically produced by a wide variety of bacteria, as an intracellular energy reserve in the form of homo- and copolymers of [R]-β-hydroxyalkanoic acids, depending on the C source used for microorganism growth, when the cells are grown under stressing conditions. In this paper we present microbiological accumulation of poly-3-hydroxyoctanoate (PHO) by using a consortium of bacterial strains, Pseudomonas putida and Bacillus subtilis, in a rate of 3:1, grown on a fermentation medium based on sodium octanoate as the sole carbon source. The experiments performed in the above mentioned conditions led to the following results: from 18.70 g sodium octanoate (7.72 g/L in the fermentation medium) used up during the bioprocess, 3.93-3.96 g/L dry bacterial biomass and 1.834 - 1.884 g/L PHA, containing 85.83 - 86.8% PHO, were obtained.

scholarly journals Bacterial Degradation of N,N-Diethyl-m-Toluamide (DEET): Cloning and Heterologous Expression of DEET Hydrolase

2007 ◽  
Vol 73 (9) ◽  
pp. 3105-3108 ◽  
Author(s):  
Giomar Rivera-Cancel ◽  
Daniela Bocioaga ◽  
Anthony G. Hay
Keyword(s):  
Carbon Source ◽  
Heterologous Expression ◽  
Pseudomonas Putida ◽  
Sole Carbon Source ◽  
Bacterial Degradation ◽  
Ring Cleavage

ABSTRACT Pseudomonas putida DTB grew aerobically with N,N-diethyl-m-toluamide (DEET) as a sole carbon source, initially breaking it down into 3-methylbenzoate and diethylamine. The former was further metabolized via 3-methylcatechol and meta ring cleavage. A gene from DTB, dthA, was heterologously expressed and shown to encode the ability to hydrolyze DEET into 3-methylbenzoate and diethylamine.

Biosurfactant production by Rhodococcus erythropolis grown on glycerol as sole carbon source

1996 ◽  
Vol 131 (1-3) ◽  
pp. 880-886
Author(s):  
Elisa M. P. Ciapina ◽  
Walber C. Melo ◽  
Lidia M. M. Santa Anna ◽  
Alexandre S. Santos ◽  
Denise M. G. Freire ◽  
...  
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Rhodococcus Erythropolis ◽  
Biosurfactant Production

scholarly journals Host engineering for improved valerolactam production in Pseudomonas putida

2019 ◽  
Author(s):  
Mitchell G. Thompson ◽  
Luis E. Valencia ◽  
Jacquelyn M. Blake-Hedges ◽  
Pablo Cruz-Morales ◽  
Alexandria E. Velasquez ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Carbon Source ◽  
Pseudomonas Putida ◽  
Aromatic Compounds ◽  
Sole Carbon Source ◽  
Carbon Sources ◽  
Shotgun Proteomics ◽  
Rich Media ◽  
Amide Hydrolase

ABSTRACTPseudomonas putida is a promising bacterial chassis for metabolic engineering given its ability to metabolize a wide array of carbon sources, especially aromatic compounds derived from lignin. However, this omnivorous metabolism can also be a hindrance when it can naturally metabolize products produced from engineered pathways. Herein we show that P. putida is able to use valerolactam as a sole carbon source, as well as degrade caprolactam. Lactams represent important nylon precursors, and are produced in quantities exceeding one million tons per year[1]. To better understand this metabolism we use a combination of Random Barcode Transposon Sequencing (RB-TnSeq) and shotgun proteomics to identify the oplBA locus as the likely responsible amide hydrolase that initiates valerolactam catabolism. Deletion of the oplBA genes prevented P. putida from growing on valerolactam, prevented the degradation of valerolactam in rich media, and dramatically reduced caprolactam degradation under the same conditions. Deletion of oplBA, as well as pathways that compete for precursors L-lysine or 5-aminovalerate, increased the titer of valerolactam from undetectable after 48 hours of production to ~90 mg/L. This work may serve as a template to rapidly eliminate undesirable metabolism in non-model hosts in future metabolic engineering efforts.

Naphthalene Degradation and Biosurfactant Activity by Bacillus cereus 28BN

2005 ◽  
Vol 60 (7-8) ◽  
pp. 577-582 ◽  
Author(s):  
Borjana Tuleva ◽  
Nelly Christova ◽  
Bojidar Jordanov ◽  
Boryana Nikolova-Damyanova ◽  
Petar Petrov
Keyword(s):  
Surface Tension ◽  
Carbon Source ◽  
Thin Layer ◽  
Bacillus Cereus ◽  
Sole Carbon Source ◽  
Stationary Growth ◽  
Spectra Analysis ◽  
Naphthalene Degradation ◽  
Environmental Technologies ◽  
Layer Chromatography

Biosurfactant activity and naphthalene degradation by a new strain identified as Bacillus cereus 28BN were studied. The strain grew well and produced effective biosurfactants in the presence of n-alkanes, naphthalene, crude oil and vegetable oils. The biosurfactants were detected by the surface tension lowering of the medium, thin layer chromatography and infrared spectra analysis. With (2%) naphthalene as the sole carbon source, high levels of rhamnolipids at a concentration of 2.3 g l-1 were determined in the stationary growth. After 20 d of incubation 72 ± 4% of the initial naphthalene was degraded. This is the first report for a Bacillus cereus rhamnolipid producing strain that utilized naphthalene under aerobic conditions. The strain looks promising for application in environmental technologies.

Biosurfactant Production by Rhodococcus erythropolis Grown on Glycerol As Sole Carbon Source

2006 ◽  
pp. 880-886 ◽  
Author(s):  
Elisa M. P. Ciapina ◽  
Walber C. Melo ◽  
Lidia M. M. Santa Anna ◽  
Alexandre S. Santos ◽  
Denise M. G. Freire ◽  
...  
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Rhodococcus Erythropolis ◽  
Biosurfactant Production

scholarly journals Surface active agent production from olive oil in high salt conditions and its process optimization

2012 ◽  
Vol 14 (4) ◽  
pp. 30-34 ◽  
Author(s):  
Mojtaba Taran ◽  
Elham Mohamadian ◽  
Sahand Asadi ◽  
Salar Bakhtiyari
Keyword(s):  
Carbon Source ◽  
Olive Oil ◽  
Yeast Extract ◽  
Surface Active Agent ◽  
Active Agent ◽  
Culture Conditions ◽  
High Salt ◽  
Biosurfactant Production ◽  
Amphiphilic Macromolecules ◽  
Surface Active

Abstract Microbial surfactants or biosurfactants are surface active amphiphilic macromolecules that are produced by a number of microorganisms (bacteria, yeast and fungi). These compounds have extensive application in various industries especially in food, pharmaceutical and oil industry. The aim of this paper is to optimize the culture conditions for the biosurfactant production from olive oil by a novel halophilic isolate microorganism. The Taguchi experimental design methodology based analysis of olive oil as carbon source, yeast extract as nitrogen source and KH2PO4 as phosphorus source revealed that the olive oil and yeast extract significantly affect biosurfactant production in high salt conditions. Maximum biosurfactant (E24= 40%) produced in the presence of 4% (v/v) olive oil, 0.2% (w/v) yeast extract, and 0.002% (w/v) KH2PO4 . In conclusion, halophilic archaeon Haloarcula sp. IRU1 could be a potential microorganism for the production of biosurfactant from olive oil as carbon source in high salt conditions. The optimal parameters obtained during the optimization process were: olive oil 4%, yeast extract 0.4% and KH2PO4 0.004%.

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