Cytotoxic Activity and Apoptosis Induction by Gaillardin

2013 ◽  
Vol 68 (3-4) ◽  
pp. 108-112 ◽  
Author(s):  
Maryam Hamzeloo Moghadam ◽  
Farzaneh Naghibi ◽  
Azadeh Atoofi ◽  
Mitra Asgharian Rezaie ◽  
Mahboobeh Irani ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Breast Adenocarcinoma ◽  
Adenocarcinoma Cell Line ◽  
Cell Lung Carcinoma ◽  
Adenocarcinoma Cell ◽  
Mcf 7

Cytotoxic activity of gaillardin, a sesquiterpene lactone isolated from Inula oculus-christi L. (Asteraceae), was assessed in the human breast adenocarcinoma cell line MCF-7, human hepatocellular carcinoma cell line HepG-2, human non-small cell lung carcinoma cell line A-549, and human colon adenocarcinoma cell line HT-29, resulting in IC50 values of 6.37, 6.20, 4.76, and 1.81 μg/mL, respectively, in the microculture tetrazolium-formazan MTT assay. In vitro apoptosis-inducing properties of gaillardin were also evaluated in MCF-7 cells with the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nickend labeling (TUNEL) assay. The results suggest gaillardin as a candidate for further studies in cancer therapy

scholarly journals Pit-1/GHF-1 and GH expression in the MCF-7 human breast adenocarcinoma cell line

2002 ◽  
Vol 173 (1) ◽  
pp. 161-167 ◽  
Author(s):  
C Gil-Puig ◽  
M Blanco ◽  
T Garcia-Caballero ◽  
C Segura ◽  
R Perez-Fernandez
Keyword(s):  
Cell Proliferation ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Rt Pcr ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Human Breast Adenocarcinoma ◽  
Mcf 7

GH expression in mammary tumors has been related to the increase and spreading of cell proliferation. Using the MCF-7 human breast adenocarcinoma cell line, it has been demonstrated that autocrine GH-stimulated mammary carcinoma cell proliferation decreased the apoptosis rate and enhanced cell spreading. Surprisingly, no data are available about the presence of Pit-1 (the main pituitary regulator of GH) or GH expression in this cell line. Using RT-PCR, Western blot and immunohistochemistry, we have demonstrated the presence of both mRNA coding Pit-1 and GH as well as Pit-1 and GH protein in the MCF-7 cell line. These data could imply that Pit-1 may be an adequate target to inhibit breast cell proliferation.

scholarly journals Novel Synthesis Method of Micronized Ti-Zeolite Na-A and Cytotoxic Activity of Its Silver Exchanged Form

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
H. F. Youssef ◽  
W. H. Hegazy ◽  
H. H. Abo-almaged ◽  
G. T. El-Bassyouni
Keyword(s):  
Cytotoxic Activity ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Synthesis Method ◽  
Carcinoma Cell Line ◽  
Breast Adenocarcinoma ◽  
Synthetic Process ◽  
Colon Cell ◽  
Colon Cell Line

The core-shell method is used as a novel synthetic process of micronized Ti-Zeolite Na-A which involves calcination at 700°C of coated Egyptian Kaolin with titanium tetrachloride in acidic medium as the first step. The produced Ti-coated metakaolinite is subjected to microwave irradiation at low temperature of 80°C for 2 h. The prepared micronized Ti-containing Zeolites-A (Ti-Z-A) is characterized by FTIR, XRF, XRD, SEM, and EDS elemental analysis. Ag-exchanged form of Ti-Z-Ag is also prepared and characterized. The Wt% of silver exchanged onto the Ti-Zeolite structure was determined by atomic absorption spectra. Thein vitrocytotoxic activity of Ti-Z-Ag against human hepatocellular carcinoma cell line (HePG2), colon cell line carcinoma (HCT116), lung carcinoma cell line (A549), and human Caucasian breast adenocarcinoma (MCF7) is reported. The results were promising and revealed that the exchanged Ag form of micronized Ti-Zeolite-A can be used as novel antitumor drug.

Glargine Promotes Proliferation of Breast Adenocarcinoma Cell Line MCF-7 via AKT Activation

2011 ◽  
Vol 43 (08) ◽  
pp. 519-523 ◽  
Author(s):  
J.-A. Teng ◽  
R.-L. Hou ◽  
D.-L. Li ◽  
R.-P. Yang ◽  
J. Qin
Keyword(s):  
Cell Line ◽  
Breast Adenocarcinoma ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Akt Activation ◽  
Mcf 7

Synthesis, Characterization and Biological Evaluation of Novel Triazolyl - Acridine Derivatives as Cytotoxic Agents

2021 ◽  
pp. 5101-5107
Author(s):  
Mandeep Singh ◽  
D. N Prasad ◽  
Supriya Agnihotri
Keyword(s):  
Cell Line ◽  
Biological Evaluation ◽  
Biologically Active ◽  
Cytotoxic Agents ◽  
Breast Adenocarcinoma ◽  
Dependent Manner ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Ht 29 ◽  
Mcf 7

Acridine and Triazols both are biologically active heterocyclic rings with cytotoxic potential. Triazolyl- acridine adduct attract the attention in the field of medicinal chemistry. Here we synthesized a series of triazolyl- acridine compounds by the appropriate procedures. Structure of all synthesized compounds was confirmed by the various spectroscopic methods e.g. FT-IR, proton NMR and Mass spectrograms. All synthesized triazolyl-acirdines compounds were assayed in-vitro for cytotoxic activity against MCF-7 (human breast adenocarcinoma cell line) and HT-29 (human colon adenocarcinoma cell line) cells by MTT- assay. All target compounds shows increasing activity in dose dependent manner. However MPSP-9 was sensitive against MCF-7 but compound MPSP-1 was most sensitive with minimum inhibitory concentration (IC50 value) against MCF-7 and HT-29 both cell lines.

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