scholarly journals The Progestin Receptor Interactome in the Female Mouse Hypothalamus: Interactions with Synaptic Proteins Are Isoform Specific and Ligand Dependent

eNeuro ◽  
2017 ◽  
Vol 4 (5) ◽  
pp. ENEURO.0272-17.2017 ◽  
Author(s):  
Kalpana D. Acharya ◽  
Sabin A. Nettles ◽  
Katherine J. Sellers ◽  
Dana D. Im ◽  
Moriah Harling ◽  
...  
Keyword(s):  
Female Mouse ◽  
Synaptic Proteins ◽  
Progestin Receptor

scholarly journals Dopamine‐induced interactions of female mouse hypothalamic proteins with progestin receptor‐A in the absence of hormone

2020 ◽  
Vol 32 (10) ◽  
Author(s):  
Kalpana D. Acharya ◽  
Sabin A. Nettles ◽  
Cheryl F. Lichti ◽  
Katherine Warre‐Cornish ◽  
Lucia Dutan Polit ◽  
...  
Keyword(s):  
Female Mouse ◽  
Progestin Receptor

scholarly journals Estradiol Preferentially Induces Progestin Receptor-A (PR-A) Over PR-B in Cells Expressing Nuclear Receptor Coactivators in the Female Mouse Hypothalamus

eNeuro ◽  
2015 ◽  
Vol 2 (4) ◽  
pp. ENEURO.0012-15.2015 ◽  
Author(s):  
Kalpana D. Acharya ◽  
Sarah D. Finkelstein ◽  
Elizabeth P. Bless ◽  
Sabin A. Nettles ◽  
Biserka Mulac-Jericevic ◽  
...  
Keyword(s):  
Nuclear Receptor ◽  
Female Mouse ◽  
Progestin Receptor ◽  
Nuclear Receptor Coactivators ◽  
In Cells

scholarly journals RFCM-PALM: In-Silico Prediction of S-Palmitoylation Sites in the Synaptic Proteins for Male/Female Mouse Data

2021 ◽  
Vol 22 (18) ◽  
pp. 9901
Author(s):  
Soumyendu Sekhar Bandyopadhyay ◽  
Anup Kumar Halder ◽  
Monika Zaręba-Kozioł ◽  
Anna Bartkowiak-Kaczmarek ◽  
Aviinandaan Dutta ◽  
...  
Keyword(s):  
Female Mouse ◽  
Critical Role ◽  
Synaptic Proteins ◽  
Post Translational Modification ◽  
Knock Out ◽  
Heuristic Strategy ◽  
Physicochemical Features ◽  
Consensus Strategy ◽  
Reversible Covalent ◽  
The Individual

S-palmitoylation is a reversible covalent post-translational modification of cysteine thiol side chain by palmitic acid. S-palmitoylation plays a critical role in a variety of biological processes and is engaged in several human diseases. Therefore, identifying specific sites of this modification is crucial for understanding their functional consequences in physiology and pathology. We present a random forest (RF) classifier-based consensus strategy (RFCM-PALM) for predicting the palmitoylated cysteine sites on synaptic proteins from male/female mouse data. To design the prediction model, we have introduced a heuristic strategy for selection of the optimum set of physicochemical features from the AAIndex dataset using (a) K-Best (KB) features, (b) genetic algorithm (GA), and (c) a union (UN) of KB and GA based features. Furthermore, decisions from best-trained models of the KB, GA, and UN-based classifiers are combined by designing a three-star quality consensus strategy to further refine and enhance the scores of the individual models. The experiment is carried out on three categorized synaptic protein datasets of a male mouse, female mouse, and combined (male + female), whereas in each group, weighted data is used as training, and knock-out is used as the hold-out set for performance evaluation and comparison. RFCM-PALM shows ~80% area under curve (AUC) score in all three categories of datasets and achieve 10% average accuracy (male—15%, female—15%, and combined—7%) improvements on the hold-out set compared to the state-of-the-art approaches. To summarize, our method with efficient feature selection and novel consensus strategy shows significant performance gains in the prediction of S-palmitoylation sites in mouse datasets.

Progestin Receptor Cells in the 8-Day-Old Male and Female Mouse Cerebral Cortex: Autoradiographic Evidence for a Sexual Dimorphism in Target Cell Number*

Endocrinology ◽  
1991 ◽  
Vol 128 (1) ◽  
pp. 87-95 ◽  
Author(s):  
PAUL J. SHUGHRUE ◽  
WALTER E. STUMPF ◽  
WALTER ELGER ◽  
PAUL-EBERHARD SCHULZE ◽  
MADHABANANDA SAR
Keyword(s):  
Cerebral Cortex ◽  
Sexual Dimorphism ◽  
Target Cell ◽  
Female Mouse ◽  
Cell Number ◽  
Male And Female ◽  
Receptor Cells ◽  
Progestin Receptor ◽  
Mouse Cerebral Cortex

The photoreceptor cytoskeleton visualized

1992 ◽  
Vol 50 (1) ◽  
pp. 480-481
Author(s):  
Beth Burnside
Keyword(s):  
Outer Segment ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Cell Polarization ◽  
Synaptic Proteins ◽  
Cell Functions ◽  
Vertebrate Photoreceptor ◽  
Numerous Cell ◽  
Turn Over

The vertebrate photoreceptor provides a drammatic example of cell polarization. Specialized to carry out phototransduction at its distal end and to synapse with retinal interneurons at its proximal end, this long slender cell has a uniquely polarized morphology which is reflected in a similarly polarized cytoskeleton. Membranes bearing photopigment are localized in the outer segment, a modified sensory cilium. Sodium pumps which maintain the dark current critical to photosensory transduction are anchored along the inner segment plasma membrane between the outer segment and the nucleus.Proximal to the nucleus is a slender axon terminating in specialized invaginating synapses with other neurons of the retina. Though photoreceptor diameter is only 3-8u, its length from the tip of the outer segment to the synapse may be as great as 200μ. This peculiar linear cell morphology poses special logistical problems and has evoked interesting solutions for numerous cell functions. For example, the outer segment membranes turn over by means of a unique mechanism in which new disks are continuously added at the proximal base of the outer segment, while effete disks are discarded at the tip and phagocytosed by the retinal pigment epithelium. Outer segment proteins are synthesized in the Golgi near the nucleus and must be transported north through the inner segment to their sites of assembly into the outer segment, while synaptic proteins must be transported south through the axon to the synapse.The role of the cytoskeleton in photoreceptor motile processes is being intensely investigated in several laboratories.

The effect of acute immobilization stress on the expression of synaptic proteins in serotonin transporter deficient mice

2007 ◽  
Vol 40 (05) ◽  
Author(s):  
SL Nietzer ◽  
AG Schmitt ◽  
G Ortega ◽  
C Kriegebaum ◽  
L Gutknecht ◽  
...  
Keyword(s):  
Serotonin Transporter ◽  
Immobilization Stress ◽  
Synaptic Proteins ◽  
Deficient Mice

Neurotransmitter systems of female mouse brain during growth of malignant melanoma modeled on the background of chronic pain

2018 ◽  
pp. 49-55
Author(s):  
О.И. Кит ◽  
И.М. Котиева ◽  
Е.М. Франциянц ◽  
И.В. Каплиева ◽  
Л.К. Трепитаки ◽  
...  
Keyword(s):  
Chronic Pain ◽  
Cerebral Cortex ◽  
Malignant Melanoma ◽  
Sciatic Nerve ◽  
Female Mouse ◽  
Combined Effects ◽  
Malignant Growth ◽  
Course Of Disease ◽  
The Brain ◽  
Melanoma Growth

Известно, что биогенные амины (БА) участвуют в злокачественном росте, их уровень изменяется в ЦНС при болевом воздействии, однако исследований о сочетанном влиянии хронической боли (ХБ) и онкопатологии на динамику БА в головном мозге не проводилось. Цель: изучить особенности баланса БА в коре головного мозга в динамике роста меланомы, воспроизведенной на фоне ХБ. Материалы и методы. Работа выполнена на 64 мышах-самках, весом 21-22 г. Животным основной группы меланому В16/F10 перевивали под кожу спины через 2 недели после перевязки седалищных нервов. Группой сравнения служили мыши с меланомой без боли. Уровни БА: адреналина, норадреналина, дофамина (ДА), серотонина (5-НТ), гистамина, а также 5-ОИУК определяли методом иммуноферментного анализа. Результаты. У мышей с ХБ уменьшается содержание большинства БА, однако уровень ДА не изменяется. Метаболизм 5-НТ происходит с участием МАО. Развитие меланомы сопровождается увеличением содержания ДА и 5-НТ, тогда как МАО - ингибируется. Направленность сдвигов БА при развитии меланомы на фоне ХБ оказалась практически такой же, как и без неё. В то же время ХБ ограничивает накопление 5-НТ в коре мозга при меланоме, что сопровождается более агрессивным её течением. Выводы. ХБ ограничивает включение стресс-лимитирующих механизмов в головном мозге при развитии меланомы у мышей, что приводит к более агрессивному течению злокачественного процесса. Biogenic amines (BA) are known to be involved in malignant growth, and their CNS levels change in pain; however, there are no studies of combined effects of chronic pain (CP) and cancer on BA dynamics in the brain. Aim: To study features of BA balance in the cerebral cortex during melanoma growth associated with CP. Material and methods. The study included 64 female mice weighing 21-22 g. In the main groups, B16/F10 melanoma was transplanted under the skin of the back two weeks following sciatic nerve ligation. Mice with melanoma without pain were used as the control. Concentrations of BA: adrenaline, noradrenaline, dopamine (DA), serotonin (5-HT), histamine and 5-HIAA were measured with ELISA. Results. Concentrations of BAs decreased in mice with CP although DA levels did not change. 5-HT metabolism involved MAO. The development of melanoma was accompanied by increases in DA and 5-HT whereas MAO was inhibited. The direction of BA changes during the development of melanoma was the same with and without CP. At the same time, CP with melanoma limited accumulation of 5-HT in the cerebral cortex, which resulted in even more aggressive course of cancer. Conclusion. CP restricted the activation of cerebral stress-limiting mechanisms during the development of melanoma in mice, which resulted in a more aggressive course of disease.

Sign in / Sign up

Export Citation Format

Share Document