scholarly journals Noradrenergic neurons from the locus ceruleus in dissociated cell culture: culture methods, morphology, and electrophysiology

1986 ◽  
Vol 6 (11) ◽  
pp. 3229-3241 ◽  
Author(s):  
S Masuko ◽  
Y Nakajima ◽  
S Nakajima ◽  
K Yamaguchi
Keyword(s):  
Cell Culture ◽  
Locus Ceruleus ◽  
Noradrenergic Neurons ◽  
Culture Methods ◽  
Dissociated Cell Culture

Cell Culture Methods to Evaluate the Biocompatibility of Implant Materials

1992 ◽  
Vol 20 (1) ◽  
pp. 52-60
Author(s):  
Gabriela Ciapetti ◽  
Elisabetta Cenni ◽  
Daniela Cavedagna ◽  
Loredana Pratelli ◽  
Arturo Pizzoferrato
Keyword(s):  
Cell Culture ◽  
Quantitative Data ◽  
Cell Function ◽  
Culture Methods ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Biological Compatibility ◽  
Compatibility Of Materials ◽  
Cell Culture Methods

Cell culture techniques are usually used in the field of biomaterials research and development in order to detect toxic components. Morphological assays are the most widely used methods and give the very first information about the biological compatibility of materials. Cell function assays give more quantitative data, but the comparison of data between different laboratories is difficult. Some of the cell culture methods that are used for biocompatibility studies are described briefly here, and results from our laboratory are reported. Despite some inherent limitations of the cell culture techniques, they are an accurate and reliable method of predicting the biological compatibility of materials to be implanted in vivo.

Preparation of Porous Organically-Modified Silicate Hybrids for Cell Culture Matrix

2007 ◽  
Vol 330-332 ◽  
pp. 1177-1180 ◽  
Author(s):  
Kanji Tsuru ◽  
Satoshi Hayakawa ◽  
Yuki Shirosaki ◽  
T. Okayama ◽  
K. Kataoka ◽  
...  
Keyword(s):  
Cell Culture ◽  
Mammalian Cells ◽  
Pore Diameter ◽  
Sol Gel ◽  
Three Dimensional ◽  
Culture Methods ◽  
Organically Modified ◽  
Dimensional Cell

Porous & rubbery organic-inorganic hybrids were synthesized from tetraethoxysilane (TEOS) and polydimethylsiloxane (PDMS) through a sol-gel route using sieved sucrose granules as a porogen. The porous hybrids with a high content of PDMS behaved like polymer sponge. The porosity was over 90% irrespective of the hybrid composition and the pore diameter ranged from 100 to 500 μm. In the three-dimensional cell culture, mammalian cells were well cultured in the porous hybrids. The present results indicate that the hybrids may be a promising scaffold for developing such functional culture methods.

Abstract P469: Scale-up And Characterization Of Therapeutic Mesenchymal Stromal Cell-derived Extracellular Vesicles

2021 ◽  
Vol 129 (Suppl_1) ◽  
Author(s):  
Selen Uman ◽  
Jason A Burdick
Keyword(s):  
Cell Culture ◽  
Extracellular Vesicles ◽  
Animal Studies ◽  
Scale Up ◽  
Spinner Flask ◽  
Perfusion Bioreactor ◽  
Large Animal ◽  
Culture Methods ◽  
Therapeutic Benefits ◽  
Cell Culture Methods

Introduction: Early studies have shown therapeutic benefits of mesenchymal stromal cells (MSCs) in cardioprotection due to their angiogenic, proliferative, anti-apoptotic and anti-inflammatory properties, which are now attributed to secreted factors such as extracellular vesicles (EVs). While MSC-EVs have shown promise in small animals for cardiovascular therapies, large animal studies are required to evaluate the therapeutic benefit of MSC-EVs for clinical translation. One of the biggest challenges for large animal studies is the need to generate clinically-relevant quality and quantity of EVs without batch-to-batch variations that could compromise efficacy. This study aims to explore three different cell culture methods (traditionally-used tissue culture plates (TCP), 3-D printed bioscaffolds in a perfusion system (P), and microcarriers in dynamic spinner flask conditions (M)) to scale-up the production of MSC-EVs across four different biological donors and rigorously investigate EV yield, size, shape, and content. Methods: MSCs were isolated from the iliac crest of four different Yucatan minipigs using heparinized syringes, and cells were expanded to passage four, at which point they were seeded onto the respective cell culture methods. EVs were collected from conditioned medium (CM) via differential ultracentrifugation. EV size, distribution, yield, and protein concentration were studied using Nanoparticle Tracking Analysis (NTA) and microBCA assays. Results: Both perfusion bioreactor and spinner flask systems enabled sustained maintenance of large numbers of cells. Across biological donors and fabrication methods, modes remained within 50-150 nm and were not statistically different. Microcarrier-based spinner flasks and perfusion bioreactor set-ups both improved EV yield, up to 6 times in efficiency. Ongoing research focuses on examining differences in EV content across biological donors using RNA-sequencing and proteomics.

Altered TNF-α, glucose, insulin, and amino acids in islets of Langerhans cultured in a microgravity model system

2001 ◽  
Vol 280 (1) ◽  
pp. E92-E102 ◽  
Author(s):  
Brian W. Tobin ◽  
Sandra K. Leeper-Woodford ◽  
Brian B. Hashemi ◽  
Scott M. Smith ◽  
Clarence F. Sams
Keyword(s):  
Cell Culture ◽  
Pancreatic Islets ◽  
Islets Of Langerhans ◽  
Three Dimensional ◽  
Model System ◽  
Culture Methods ◽  
Static Culture ◽  
Pancreatic Islets Of Langerhans ◽  
Tnf Α ◽  
Factor Α

The present studies were designed to determine effects of a microgravity model system upon lipopolysaccharide (LPS)-stimulated tumor necrosis factor-α (TNF-α) activity and indexes of insulin and fuel homeostasis of pancreatic islets of Langerhans. Islets (1,726 ± 117, 150 islet equivalent units) from Wistar-Furth rats were treated as 1) high aspect ratio vessel (HARV) cell culture, 2) HARV plus LPS, 3) static culture, and 4) static culture plus LPS. TNF-α (L929 cytotoxicity assay) was significantly increased in LPS-induced HARV and static cultures; yet the increase was more pronounced in the static culture group ( P < 0.05). A decrease in insulin concentration was demonstrated in the LPS-stimulated HARV culture ( P< 0.05). We observed a greater glucose concentration and increased disappearance of arginine in islets cultured in HARVs. Although nitrogenous compound analysis indicated a ubiquitous reliance on glutamine in all experimental groups, arginine was converted to ornithine at a twofold greater rate in the islets cultured in the HARV microgravity model system ( P < 0.05). These studies demonstrate alterations in LPS-induced TNF-α production of pancreatic islets of Langerhans, favoring a lesser TNF activity in the HARV. These alterations in fuel homeostasis may be promulgated by gravity-averaged cell culture methods or by three-dimensional cell assembly.

scholarly journals Toxicity and Teratogenic Effects of Zinc Sulfide Nanoparticles on Chick Embryo and Chick Fibroblast Cell Culture

2019 ◽  
Vol 25 (4) ◽  
pp. 270-281
Author(s):  
Asadollah Asadi ◽  
◽  
Arash Abdolmaleki ◽  
◽  
Keyword(s):  
Cell Culture ◽  
Zinc Sulfide ◽  
Morphological Changes ◽  
Lethal Dose ◽  
Fibroblast Cell ◽  
Zns Nanoparticles ◽  
Culture Methods ◽  
Study Results ◽  
Zinc Sulfide Nanoparticles ◽  
Fibroblastic Cells

Aims Nanoparticles (particles with a diameter of 10-500 nm) are currently used in the cosmetics industry as well as for pharmaceuticals, diagnostic imaging, and tissue engineering. Since these nanoparticles are used in industry and drug delivery, they can also be used by pregnant women. Thus, the current study investigated the teratogenic and cytotoxic effects of Zinc Sulfide (ZnS) nanoparticles on the embryo and their fibroblastic cell culture. Methods & Materials Zinc sulfide (ZnS) nanoparticles were synthesized. Then, nanoparticles at the concentrations of 5, 10, 15, 30, and 40 mg/mL/egg were injected into the air sac of the eggs in three replicates on the third day of incubation. Next, the treated and control eggs, on day 19 of incubation were opened, and embryos were weighted, and the relevant mortality rate was recorded. Fibroblast cells were isolated, cultured, and treated from the control embryo, and morphological changes and cell survival percentages were recorded. Findings The obtained results revealed that the embryos’ survival rate depends on the nanoparticle concentration. As a result, at the highest concentration, only 36.32% of the embryos survived, and the lethal dose 50% (LD50) was equal to 32.47 mg/egg. Morphological study of the treated embryos club foot and skeletal staining suggested the deletion of caudal vertebrate. The cytotoxicity study results of ZnS nanoparticles on fibroblastic cells indicated the survival fractions of 88.45%, 68.75%, and 49.32%, respectively, and its IC50 value was measured aas1460 μM. Conclusion The present study results suggested that ZnS nanoparticles had no significant toxic effects on the embryos and culture of chicken fibroblastic cells at low concentrations.

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