The purpose of this study was to determine changes of lipoxygenase activities, contents of antioxidants (tocopherol ?, ?-carotene and chlorophyll) fatty acids and soluble proteins, as well as, vigor in accelerated aged soybean seeds. The following soybean cultivars were used in the study: Williams 82, Goyou Kurakake and L93-7290. Subsequent to the accelerated ageing test (AAT), 23% of seeds of the cultivar Goyou Kurakake retained normal germination, while grain of the two remaining cultivars L93-7290 and Williams 82, completely lost germination ability. According to our results, the seeds of the cultivar Goyou Kurakake (the absence of lipoxygenases 2) is characterized by a significantly higher content of all observed antioxidants (tocopherol ? 2.7 mg 100g-1, ?-carotene 6.1 ?g g-1 chlorophyll 4.9 ?g g-1). The contents of (?-carotene and chlorophyll decreased after AAT in the seed of the cultivar Goyou Kurakake by 42.8% and 60.5%, respectively, while the content of tocopherol a remained the same. Furthermore, the tocopherol a content was not changed after AAT neither in the cultivar L93-7290 nor in the cultivar Williams 82. The content of ?-carotene after AAT remained the same in seeds of the cultivar Williams 82 while it decreased by 7.7% in the cultivar L93-7290. Since changes of the fatty acids content were not pronounced in the cultivar Goyou Kurakake after AAT it can be concluded that the antioxidative system had a crucial role in seeds protection against lipid peroxidation.
Moist Heat Accelerated Ageing Test of Naturally Aged Paper : Prediction of Initial Physical Properties and Degradation Rate Indicator at Room Temperature by Suspension Method
