EFFECT OF HEAT ON THE IMMUNOLOGICAL PROPERTIES OF HUMAN, BOVINE AND OVINE GROWTH HORMONE

ABSTRACT Human, bovine and sheep (ovine) growth hormone (HGH, BGH and SGH) were heated in solution at temperatures between 60 and 100 °C. The electrophoretic mobility and immunological properties, such as precipitation reactions in agar gel and haemagglutination with antiserum to untreated hormone, were studied at different degrees of heating. It was found that heat progressively reduced the immunological properties of the growth hormone; however, human growth hormone was more resistant to heat treatment than the bovine and sheep growth hormone. HGH retained precipitation properties when heated at 100° C up to 30 minutes, and reacted in the haemagglutination test when heated at 100° C for less than 60 minutes. BGH and SGH clotted at 100° C. The precipitation reaction with antiserum to BGH disappeared when BGH or SGH was heated at 70° C for more than 10 minutes. Only a weak haemagglutination reaction was retained when BGH or SGH was heated at 80° C for 15 minutes.

Download Full-text

Immunological Properties of Electrophoretic Fractions of Human Growth Hormone

Nature ◽

10.1038/194491a0 ◽

1962 ◽

Vol 194 (4827) ◽

pp. 491-492 ◽

Cited By ~ 8

Author(s):

ZVI LARON ◽

SARA ASSA

Keyword(s):

Growth Hormone ◽

Human Growth Hormone ◽

Human Growth ◽

Immunological Properties

Download Full-text

STUDIES ON A MONKEY PLACENTAL PROTEIN WITH IMMUNOCHEMICAL SIMILARITY TO HUMAN GROWTH HORMONE AND HUMAN CHORIONIC SOMATOMAMMOTROPHIN

Acta Endocrinologica ◽

10.1530/acta.0.0630736 ◽

1970 ◽

Vol 63 (4) ◽

pp. 736-746 ◽

Cited By ~ 5

Author(s):

David B. Grant ◽

Selna L. Kaplan ◽

Melvin M. Grumbach

Keyword(s):

Growth Hormone ◽

Electrophoretic Mobility ◽

Human Growth Hormone ◽

Human Growth ◽

Double Diffusion ◽

Molecular Weights ◽

Diffusion Studies ◽

Placental Protein ◽

Two Peaks ◽

Dilution Curves

ABSTRACT The properties of a monkey placental protein (monkey chorionic somatomammotrophin; MCS) which reacts with antisera directed against human growth hormone (HGH) and human chorionic somatomammotrophin (HCS) have been studied. Following application of monkey placental extracts to Sephadex G-100 and elution with 0.1 m NH4HCO3, two peaks of MCS, with molecular weights of approximately 25 000 and 50 000 were obtained. The electrophoretic mobility of MCS in acrylamide at pH 8.2–8.4 was similar to but less anodal than that of HGH or HCS. The results of immunoelectrophoretic and Ouchterlony double-diffusion studies indicated that MCS is physicochemically and immunochemically distinguishable from HGH and HCS. When tested in a radioimmunoassay for HGH, MCS gave dilution curves intermediate between those for HGH and HCS. In contrast, MCS and HGH gave similar curves in a radioimmunoassay for HCS. MCS, HCS and HGH had very similar effects on the reactions between 131I-HGH and anti-HCS or 131I-HCS and anti-HGH serum. Radioimmunoassay systems for HCS were unsuitable for estimating MCS; however, the above mentioned hybrid assay systems provide a means for approximate quantitation of MCS.

Download Full-text

IMMUNOCHEMICAL STUDIES OF HUMAN GROWTH HORMONE, OVINE PROLACTIN, BOVINE GROWTH HORMONE AND A TRYPTIC DIGEST OF BOVINE GROWTH HORMONE

Journal of Endocrinology ◽

10.1677/joe.0.0440517 ◽

1969 ◽

Vol 44 (4) ◽

pp. 517-NP ◽

Cited By ~ 1

Author(s):

KALYAN SUNDARAM ◽

M. SONENBERG

Keyword(s):

Growth Hormone ◽

Human Growth Hormone ◽

Deae Cellulose ◽

Human Growth ◽

Double Diffusion ◽

Bovine Growth Hormone ◽

Agar Gel ◽

Tryptic Digest ◽

Immunological Reactions ◽

Ovine Prolactin

SUMMARY Agar gel double diffusion and immunoelectrophoresis of human growth hormone (HGH), ovine prolactin, bovine growth hormone (BGH) and a tryptic digest of BGH (TBGH) were performed. Antisera to BGH, TBGH, HGH and ovine prolactin were used for the tests. Both BGH and TBGH were found to contain material that precipitated with antiserum to ovine prolactin. Immunological analysis of various fractions of BGH and TBGH separated by chromatography on DEAE-cellulose showed that BGH and TBGH were eluted before prolactin. No differences between BGH and TBGH in their immunological reactions were found. HGH did not cross-react with BGH, TBGH or ovine prolactin.

Download Full-text

IMMUNOCHEMICAL STUDIES OF HUMAN PITUITARY GROWTH HORMONE

Acta Endocrinologica ◽

10.1530/acta.0.0400311 ◽

1962 ◽

Vol 40 (2) ◽

pp. 311-320 ◽

Cited By ~ 8

Author(s):

Zvi Laron ◽

Sara Assa

Keyword(s):

Growth Hormone ◽

Human Growth Hormone ◽

Haemagglutination Inhibition ◽

Rabbit Antiserum ◽

Human Growth ◽

Human Albumin ◽

Agar Gel ◽

Human Pituitary ◽

Pituitary Glands ◽

Fraction I

ABSTRACT Human growth hormone (HGH) prepared from human pituitary glands by the method of Raben (1959) showed two components on agar gel electrophoresis. Fraction I corresponded to a slow alpha-globulin, and fraction II had a mobility between alpha and beta-globulin. On agar gel immunoelectrophoresis using HGH and rabbit antiserum to HGH, these two components showed immunological identity. By using agar gel immunoelectrophoresis and the haemagglutination technique HGH preparations of both Raben and Li type were shown to be contaminated with human albumin. Antiserum to human growth hormone containing also antibodies against human albumin was purified by adsorption with human albumin. It is postulated that part of the difficulties encountered in the use of the haemagglutination-inhibition technique to determine growth hormone concentration in serum, is due to impurities in the human growth hormone preparations and antisera to these preparations in use.

Download Full-text

IMMUNOCHEMICAL STUDIES ON THE HETEROGENEITY OF HUMAN GROWTH HORMONE (HGH)

Acta Endocrinologica ◽

10.1530/acta.0.0600101 ◽

1969 ◽

Vol 60 (1) ◽

pp. 101-111 ◽

Cited By ~ 1

Author(s):

W. Rohde ◽

G. Dörner

Keyword(s):

Growth Hormone ◽

Human Growth Hormone ◽

Extraction Procedure ◽

Gel Filtration ◽

Human Growth ◽

Double Diffusion ◽

Active Components ◽

Agar Gel ◽

Human Pituitary ◽

Starch Gel

ABSTRACT The specific antigenic patterns of human growth hormone in various human pituitary extracts were determined by analysis in gel filtration, in starch gel and agar gel immunoelectrophoresis. The analysis of pituitary extracts by gel filtration on Sephadex G-200 indicates that HGH complexes with different molecular weights were present in all tested pituitary extracts. The rate of formation of high molecular HGH was dependent on the extraction procedure used. Altogether seven immunologically active components of HGH were detected by analysis of crude pituitary extracts in starch gel immunoelectrophoresis. The number and the rate of formed HGH components were dependent on the solvent used for the extraction of pituitary homogenates and on the sequence of extraction procedures. The electrophoretical patterns obtained were reproducible. The electrophoretical behaviour of HGH of various pituitary extracts in agar gel also suggested the presence of multiple components. Three different positions were observed between the α1- and β1-globulins. The analysis of pituitary extracts in the two dimensional double diffusion technique in agar gel using an absorbed anti-HGH-serum gave evidence of the antigenic homogeneity of HGH in the different preparations. It is concluded that the electrophoretic heterogeneity of pituitary growth hormone preparations is already determined by the first step of the extraction.

Download Full-text