STUDIES ON A MONKEY PLACENTAL PROTEIN WITH IMMUNOCHEMICAL SIMILARITY TO HUMAN GROWTH HORMONE AND HUMAN CHORIONIC SOMATOMAMMOTROPHIN

ABSTRACT The properties of a monkey placental protein (monkey chorionic somatomammotrophin; MCS) which reacts with antisera directed against human growth hormone (HGH) and human chorionic somatomammotrophin (HCS) have been studied. Following application of monkey placental extracts to Sephadex G-100 and elution with 0.1 m NH4HCO3, two peaks of MCS, with molecular weights of approximately 25 000 and 50 000 were obtained. The electrophoretic mobility of MCS in acrylamide at pH 8.2–8.4 was similar to but less anodal than that of HGH or HCS. The results of immunoelectrophoretic and Ouchterlony double-diffusion studies indicated that MCS is physicochemically and immunochemically distinguishable from HGH and HCS. When tested in a radioimmunoassay for HGH, MCS gave dilution curves intermediate between those for HGH and HCS. In contrast, MCS and HGH gave similar curves in a radioimmunoassay for HCS. MCS, HCS and HGH had very similar effects on the reactions between 131I-HGH and anti-HCS or 131I-HCS and anti-HGH serum. Radioimmunoassay systems for HCS were unsuitable for estimating MCS; however, the above mentioned hybrid assay systems provide a means for approximate quantitation of MCS.

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Further Purification and Characterization of a Placental Protein with Immunological Similarity to Human Growth Hormone

Nature ◽

10.1038/2081214a0 ◽

1965 ◽

Vol 208 (5016) ◽

pp. 1214-1215 ◽

Cited By ~ 20

Author(s):

HENRY FRIESEN

Keyword(s):

Growth Hormone ◽

Human Growth Hormone ◽

Human Growth ◽

Purification And Characterization ◽

Placental Protein

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EFFECT OF HEAT ON THE IMMUNOLOGICAL PROPERTIES OF HUMAN, BOVINE AND OVINE GROWTH HORMONE

Acta Endocrinologica ◽

10.1530/acta.0.0460465 ◽

1964 ◽

Vol 46 (3) ◽

pp. 465-472 ◽

Cited By ~ 1

Author(s):

Zvi Laron ◽

Ariana Yed-Lekach ◽

Sara Assa ◽

Avivah Kowadlo-Silbergeld

Keyword(s):

Heat Treatment ◽

Growth Hormone ◽

Electrophoretic Mobility ◽

Human Growth Hormone ◽

Human Growth ◽

Precipitation Reaction ◽

Agar Gel ◽

Immunological Properties ◽

Haemagglutination Test ◽

Precipitation Reactions

ABSTRACT Human, bovine and sheep (ovine) growth hormone (HGH, BGH and SGH) were heated in solution at temperatures between 60 and 100 °C. The electrophoretic mobility and immunological properties, such as precipitation reactions in agar gel and haemagglutination with antiserum to untreated hormone, were studied at different degrees of heating. It was found that heat progressively reduced the immunological properties of the growth hormone; however, human growth hormone was more resistant to heat treatment than the bovine and sheep growth hormone. HGH retained precipitation properties when heated at 100° C up to 30 minutes, and reacted in the haemagglutination test when heated at 100° C for less than 60 minutes. BGH and SGH clotted at 100° C. The precipitation reaction with antiserum to BGH disappeared when BGH or SGH was heated at 70° C for more than 10 minutes. Only a weak haemagglutination reaction was retained when BGH or SGH was heated at 80° C for 15 minutes.

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In vitro effect of human growth hormone on lymphocyte transformation and lymphocyte growth factors secretion

Acta Endocrinologica ◽

10.1530/acta.0.1200745 ◽

1989 ◽

Vol 120 (6) ◽

pp. 745-752 ◽

Cited By ~ 7

Author(s):

R. M. Schimpff ◽

A. M. Repellin

Keyword(s):

Growth Hormone ◽

Human Growth Hormone ◽

Lymphocyte Transformation ◽

Human Growth ◽

Experimental Conditions ◽

Activated Lymphocytes ◽

Molecular Weights ◽

Normal Human ◽

Vitro Effect

Abstract. Cultures of human blood peripheral lymphocytes were performed in the presence or absence of human growth hormone, and also of phytohemagglutinin and normal human serum 10%. After incubation for 48 h, the supernatants were tested for their ability to promote the uptake of [3H]thymidine into lectin-activated lymphocytes. Supernatants from lymphocyte-free control samples, treated in the same manner, were assayed under the same experimental conditions. Variance analysis of the different dose-response relationships was performed. The results of these in vitro experiments confirm that physiological levels of GH inhibit the lectin-induced lymphoproliferation and that lymphocytes secrete an 'activity' able to stimulate the incorporation of [3H]thymidine into lectin activated lymphocytes. Furthermore we show that: 1) Secretion of this lymphocyte-stimulating activity is increased by physiological levels of GH; 2) This lymphocytic secretion is not radioimmunoassayable IGF-I; 3) Using fast protein liquid chromatography (FPLC), this activity appears in fractions with various molecular weights.

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IMMUNOCHEMICAL STUDIES OF HUMAN GROWTH HORMONE, OVINE PROLACTIN, BOVINE GROWTH HORMONE AND A TRYPTIC DIGEST OF BOVINE GROWTH HORMONE

Journal of Endocrinology ◽

10.1677/joe.0.0440517 ◽

1969 ◽

Vol 44 (4) ◽

pp. 517-NP ◽

Cited By ~ 1

Author(s):

KALYAN SUNDARAM ◽

M. SONENBERG

Keyword(s):

Growth Hormone ◽

Human Growth Hormone ◽

Deae Cellulose ◽

Human Growth ◽

Double Diffusion ◽

Bovine Growth Hormone ◽

Agar Gel ◽

Tryptic Digest ◽

Immunological Reactions ◽

Ovine Prolactin

SUMMARY Agar gel double diffusion and immunoelectrophoresis of human growth hormone (HGH), ovine prolactin, bovine growth hormone (BGH) and a tryptic digest of BGH (TBGH) were performed. Antisera to BGH, TBGH, HGH and ovine prolactin were used for the tests. Both BGH and TBGH were found to contain material that precipitated with antiserum to ovine prolactin. Immunological analysis of various fractions of BGH and TBGH separated by chromatography on DEAE-cellulose showed that BGH and TBGH were eluted before prolactin. No differences between BGH and TBGH in their immunological reactions were found. HGH did not cross-react with BGH, TBGH or ovine prolactin.

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IMMUNOCHEMICAL STUDIES ON THE HETEROGENEITY OF HUMAN GROWTH HORMONE (HGH)

Acta Endocrinologica ◽

10.1530/acta.0.0600101 ◽

1969 ◽

Vol 60 (1) ◽

pp. 101-111 ◽

Cited By ~ 1

Author(s):

W. Rohde ◽

G. Dörner

Keyword(s):

Growth Hormone ◽

Human Growth Hormone ◽

Extraction Procedure ◽

Gel Filtration ◽

Human Growth ◽

Double Diffusion ◽

Active Components ◽

Agar Gel ◽

Human Pituitary ◽

Starch Gel

ABSTRACT The specific antigenic patterns of human growth hormone in various human pituitary extracts were determined by analysis in gel filtration, in starch gel and agar gel immunoelectrophoresis. The analysis of pituitary extracts by gel filtration on Sephadex G-200 indicates that HGH complexes with different molecular weights were present in all tested pituitary extracts. The rate of formation of high molecular HGH was dependent on the extraction procedure used. Altogether seven immunologically active components of HGH were detected by analysis of crude pituitary extracts in starch gel immunoelectrophoresis. The number and the rate of formed HGH components were dependent on the solvent used for the extraction of pituitary homogenates and on the sequence of extraction procedures. The electrophoretical patterns obtained were reproducible. The electrophoretical behaviour of HGH of various pituitary extracts in agar gel also suggested the presence of multiple components. Three different positions were observed between the α1- and β1-globulins. The analysis of pituitary extracts in the two dimensional double diffusion technique in agar gel using an absorbed anti-HGH-serum gave evidence of the antigenic homogeneity of HGH in the different preparations. It is concluded that the electrophoretic heterogeneity of pituitary growth hormone preparations is already determined by the first step of the extraction.

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