TESTOSTERONE AND OESTRADIOL IN DOGS WITH TESTICULAR TUMOURS

1974 ◽  
Vol 77 (2) ◽  
pp. 408-416 ◽  
Author(s):  
F. Comhaire ◽  
D. Mattheeuws ◽  
A. Vermeulen
Keyword(s):  
Plasma Concentration ◽  
Sertoli Cell ◽  
Sex Steroids ◽  
Venous Blood ◽  
Plasma Testosterone ◽  
Testosterone Concentration ◽  
Peripheral Plasma ◽  
Normal Mass ◽  
The Mean ◽  
Sertoli Cell Tumours

ABSTRACT The mean peripheral plasma concentration of oestradiol was found to be increased in 3 dogs with Sertoli cell tumours and in 3 dogs with seminomas, whereas the plasma testosterone showed no difference as compared to a group of dogs without testicular neoplasia. In two thirds of the cases the concentration of oestradiol in the spermatic venous blood draining the neoplastic testes was clearly higher than in the normal dogs. The testosterone concentration in the spermatic venous blood from the tumour bearing testes was lower than in the spermatic venous blood of the contralateral partner testes in the same dogs, though not different from the spermatic venous concentration in the control dogs. There was no correlation between the presence or absence of signs of feminization and the peripheral or spermatic venous concentration of sex steroids. It is concluded that not only Sertoli cell tumours, but also seminomas can secrete increased amounts of oestrogens. This is possibly due to the presence of a larger than normal mass of tissue capable of converting testosterone, or its precursors, to oestradiol.

STIMULATORY EFFECT OF PROSTAGLANDIN F2α ON THE PLASMA CONCENTRATION OF TESTOSTERONE IN BULLS

1975 ◽  
Vol 66 (3) ◽  
pp. 329-338 ◽  
Author(s):  
N. B. HAYNES ◽  
H. D. HAFS ◽  
R. J. WATERS ◽  
J. G. MANNS ◽  
A. RILEY
Keyword(s):  
Plasma Concentration ◽  
Intramuscular Injection ◽  
Prostaglandin F2α ◽  
Plasma Testosterone ◽  
Testosterone Concentration ◽  
Peripheral Plasma ◽  
Prostaglandin F ◽  
The Mean

SUMMARY The concentrations of peripheral plasma testosterone were measured by radioimmunoassay in samples collected from five bulls, each given i.m. injections of 0, 5, 15, 30 and 60 mg prostaglandin F2α (PGF2α). Synchronized peaks in testosterone concentration occurred with maximum values 1–2 h after treatment. These increases of testosterone persisted significantly longer than those observed to occur as natural episodic peaks during two 24 h periods in the same bulls. The mean peak testosterone concentration after PGF2α injection was related to the dose of PGF2α, values after 60 and 30 mg doses being significantly greater than after 15 mg. The response produced by a 5 mg dose was not significant. The results indicate that intramuscular injection of PGF2α acutely stimulates synthesis and release of testosterone in bulls.

Plasma concentrations of testosterone and luteinizing hormone in rutting reindeer bulls (Rangifer tarandrus)

1980 ◽  
Vol 58 (11) ◽  
pp. 2081-2083 ◽  
Author(s):  
K.-A. Stokkan ◽  
K. Hove ◽  
W. R. Carr
Keyword(s):  
Luteinizing Hormone ◽  
Testosterone Level ◽  
Plasma Concentrations ◽  
Plasma Testosterone ◽  
Plasma Samples ◽  
Diurnal Pattern ◽  
Testosterone Concentration ◽  
Peripheral Plasma ◽  
The Mean

Concentrations of plasma testosterone and luteinizing hormone (LH) were measured in peripheral plasma from semidomestic, rutting reindeer bulls. Although the concentrations of plasma testosterone were high and showed large variations, those of LH were low and only a few episodic bursts could be detected in hourly samples taken throughout a 48-h period. The mean testosterone concentration in three bulls differed significantly and ranked the animals according to their position in a fighting hierarchy. The mean concentrations of LH did not differ significantly. Plasma samples from one reindeer bull sampled every 20 min for periods of 3 h indicated that an increment in LH concentration preceded a peak in testosterone. No diurnal pattern in testosterone concentrations could be detected, but testosterone peaks seemed to appear about every 3–4 h. The present study thus demonstrates that a series of plasma samples throughout the day is necessary to determine a true "testosterone level" in the reindeer bull.

Inhibin, gonadotrophins and sex steroids in dogs with Sertoli cell tumours

1990 ◽  
Vol 127 (2) ◽  
pp. 235-242 ◽  
Author(s):  
A. J. Grootenhuis ◽  
F. J. van Sluijs ◽  
I. A. Klaij ◽  
J. Steenbergen ◽  
M. A. Timmerman ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Sex Steroids ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Peripheral Plasma ◽  
Gonadotrophin Secretion ◽  
Secretory Products ◽  
Inhibin Subunits ◽  
Sertoli Cell Tumours

ABSTRACT Inhibin bioactivity and mRNA for inhibin subunits were measured in four dog Sertoli cell tumours and in the testes of five normal control dogs. The tumours contained increased levels of inhibin (P <0·05) and mRNA for the α and βB subunits when compared with controls, whereas the mRNA for the βA subunit was not detected in tumours or control testes. The inhibin bioactivity was associated with a 32 kDa molecule in both Sertoli cell tumours and normal dog testes; no higher molecular weight forms were found after sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Peripheral levels of immunoassayable inhibin in dogs with Sertoli cell tumours were higher than those in the controls (P = 0·01), indicating that it might be possible to use this parameter as a marker for Sertoli cell tumours. Other testicular tumours, however, might also secrete immunoactive inhibin. The increased inhibin concentrations are likely to be the cause of the suppressed peripheral levels of FSH (P <0·02). However, peripheral levels of LH (P <0·02) and testosterone (P <0·01) were also suppressed in the dogs with Sertoli cell tumours, whereas the concentrations of oestradiol in the peripheral plasma of both groups did not differ. Finally, i.v. injection of the LHRH agonist buserelin caused a significant increase in LH and testosterone in the control dogs, but not in the dogs with Sertoli cell tumours. It was concluded that secretory products from the Sertoli cell tumours suppressed pituitary gonadotrophin secretion. It is unlikely that testosterone or oestradiol play a role in this respect. FSH may be suppressed by the high levels of inhibin in tumour-bearing dogs, but it remains unclear whether inhibin or another Sertoli cell product is responsible for the unresponsiveness of the pituitary gland to LHRH and the suppression of LH. Journal of Endocrinology (1990) 127, 235–242

PLASMA CONCENTRATION OF TESTOSTERONE, DIHYDROTESTOSTERONE, TESTOSTERONE-OESTRADIOL BINDING GLOBULIN, AND PITUITARY GONADOTROPHINS IN THE SYNDROME OF MALE PSEUDO-HERMAPHRODITISM WITH TESTICULAR FEMINIZATION

1972 ◽  
Vol 70 (2) ◽  
pp. 331-341 ◽  
Author(s):  
Roland R. Tremblay ◽  
Thomas P. Foley ◽  
Pierre Corvol ◽  
In-Joo Park ◽  
Avinoam Kowarski ◽  
...  
Keyword(s):  
Plasma Concentration ◽  
Reductase Activity ◽  
Venous Blood ◽  
Follicle Stimulating Hormone ◽  
Normal Subjects ◽  
Normal Male ◽  
Testicular Feminization ◽  
Peripheral Plasma ◽  
Serum Lh ◽  
High Concentrations

ABSTRACT Twenty patients with the syndrome of testicular feminization have been studied along with a group of normal subjects of both sexes and of comparable ages. Peripheral and gonadal venous blood were analysed for their content in testosterone (T), androstenedione (Δ), dihydrotestosterone (DHT), testosterone-oestradiol binding globulin (TeBG), luteinizing hormone (LH) and follicle stimulating hormone (FSH). Normal or even higher than normal male concentrations of T, Δ, and DHT were observed. An actual secretion of the three androgens by the testis was demonstrated. Elevated levels of serum LH were found despite normal male or higher levels of T, Δ, and DHT. High concentrations of TeBG presumably could alter the dynamics of plasma androgens. The gonadal production of DHT and the normal levels of DHT in peripheral plasma shows that 5α-reductase activity is definitely present in the patients.

Peripheral plasma testosterone concentration and sexual behavior in young prenatally stressed boars

1979 ◽  
Vol 12 (5) ◽  
pp. 289-305 ◽  
Author(s):  
H.G. Kattesh ◽  
E.T. Kornegay ◽  
F.C. Gwazdauskas ◽  
J.W. Knight ◽  
H.R. Thomas
Keyword(s):  
Sexual Behavior ◽  
Plasma Testosterone ◽  
Testosterone Concentration ◽  
Peripheral Plasma ◽  
Prenatally Stressed

PLASMA TESTOSTERONE IN WOMEN IN LATE PREGNANCY AND AFTER DELIVERY

1968 ◽  
Vol 42 (3) ◽  
pp. 477-481 ◽  
Author(s):  
K. DEMISCH ◽  
J. K. GRANT ◽  
W. BLACK
Keyword(s):  
Protein Binding ◽  
Late Pregnancy ◽  
Plasma Testosterone ◽  
Testosterone Concentration ◽  
Healthy Women ◽  
The Mean

SUMMARY The concentration of testosterone was measured in the plasma of 16 healthy women in late pregnancy and in 14 of these after delivery. The mean testosterone concentration was found to be elevated in late pregnancy, although the range overlapped with the normal. There was a prompt return to normal concentrations after delivery. The significance of the results and of inactivation of testosterone by protein binding are discussed.

Virilization of a post-menopausal woman by a testosterone-secreting Leydig cell type adrenal adenoma

1981 ◽  
Vol 98 (2) ◽  
pp. 274-282 ◽  
Author(s):  
B. N. Trost ◽  
M. P. Koenig ◽  
A. Zimmermann ◽  
M. Zachmann ◽  
J. Müller
Keyword(s):  
Leydig Cell ◽  
Adrenal Adenoma ◽  
Venous Blood ◽  
Adrenal Vein ◽  
Plasma Testosterone ◽  
Menopausal Woman ◽  
Cell Type ◽  
Bilateral Oophorectomy ◽  
Testosterone Concentration ◽  
Post Menopausal

Abstract. In a 60 year old virilized woman the plasma testosterone concentration was markedly elevated, whereas the plasma cortisol and ACTH as well as urinary 17-ketosteroids, 17-hydroxycorticoids, pregnanetriol and dehydroepiandrosterone were normal. The plasma levels of LH and FSH were in the post-menopausal range. Dexamethasone suppressed the urinary 17-ketosteroids, 17-hydroxycorticoids and pregnanetriol normally, but had no effect upon the plasma testosterone. These findings led to the tentative diagnosis of an ovarian hilus cell tumour. However, bilateral oophorectomy revealed bilateral hyperthecosis without a tumour and did not result in any decrease of the plasma testosterone level. An attempt of adrenal vein catheterization succeeded only on the left side. The lack of a gradient in the testosterone concentration between blood from the left adrenal vein and a peripheral vein prompted a surgical exploration of the right adrenal gland, which led to the discovery and removal of an encapsulated Leydig cell type adenoma, characterized by Reinke's crystalloids. A testosterone concentration gradient between right adrenal and peripheral venous blood obtained intra-operatively, rapid normalization of the plasma testosterone concentration post-operatively, results of a tumour incubation study as well as the clinical outcome proved that this adenoma had been the source of the excessive androgen. A history of a late menarche and persistent menstrual irregularities together with elevated gonadotrophins in spite of excessive testosterone pointed to the possibility of a longstanding gonadotrophin-dependent gonadal cell population in the adrenal, which was fully activated only by the menopausal gonadotrophin rise.

Sex steroid concentrations in plasma from the canine deferential vein

1983 ◽  
Vol 96 (2) ◽  
pp. 223-228 ◽  
Author(s):  
P. Boulanger ◽  
M. Desaulniers ◽  
G. Bleau ◽  
K. D. Roberts ◽  
A. Chapdelaine
Keyword(s):  
Sex Steroids ◽  
Prostate Gland ◽  
Local Influence ◽  
Sex Steroid ◽  
Peripheral Plasma ◽  
Anatomical Relationship ◽  
The Mean

Sex steroid concentrations in plasma collected from the canine deferential vein were measured, after separation, by radioimmunoassay. The concentrations found were compared with those in the peripheral plasma. The mean concentrations of androstenedione, testosterone, 5α-dihydrotestosterone, 5α-androstane-3α,17β-diol, 5α-androstane-3β,17β-diol and oestradiol-17β were 13·2, 14·7, 8·9, 4·6, 8·0 and 7·5 fold higher respectively in plasma from the deferential vein than in peripheral plasma. A close anatomical relationship was found between the vasa deferentia, the deferential vein and the peripheral plasma as well as between the deferential vein and the prostate gland. These findings emphasize and extend earlier conclusions that high levels of sex steroids present in the deferential vein could have a local influence on the growth of the prostate.

HUMAN TESTIS DOES NOT SECRETE OESTRONE SULPHATE

1982 ◽  
Vol 92 (2) ◽  
pp. 185-194 ◽  
Author(s):  
F. C. W. WU ◽  
I. A. SWANSTON ◽  
T. B. HARGREAVE ◽  
D. T. BAIRD
Keyword(s):  
Isotope Dilution ◽  
Venous Blood ◽  
Human Testis ◽  
Peripheral Plasma ◽  
Oestrone Sulphate ◽  
Antecubital Vein ◽  
The Mean ◽  
Almost All ◽  
Peripheral Conversion ◽  
Venous Plasma

The concentrations of five steroids in samples of spermatic venous blood collected from 17 men undergoing ligation of varicocoeles were compared with those in samples from the antecubital vein. There was evidence of testicular secretion of testosterone, androstenedione, oestradiol-17β and oestrone, since the ratios of the mean concentrations in spermatic venous plasma to those in peripheral venous plasma were 77·2, 9·1, 28·7 and 1·6 respectively. The testicular secretion of oestrone sulphate was minimal; the ratio of the mean concentrations in spermatic and peripheral plasma was 1·07. These results support the view derived from isotope dilution studies that almost all oestrone and oestrone sulphate in the circulation is derived from peripheral conversion of other precursor steroids.

THE EFFECT OF ACTH ON PLASMA TESTOSTERONE AND ANDROSTENEDIONE CONCENTRATIONS IN PATIENTS WITH PROSTATIC CARCINOMA

1976 ◽  
Vol 81 (2) ◽  
pp. 310-320 ◽  
Author(s):  
Teresa H. Cowley ◽  
B. G. Brownsey ◽  
M. E. Harper ◽  
W. B. Peeling ◽  
K. Griffiths
Keyword(s):  
Plasma Concentration ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Endocrine Therapy ◽  
Prostatic Carcinoma ◽  
Plasma Testosterone ◽  
Testosterone Concentration ◽  
Carcinoma Of The Prostate ◽  
Specific Radioimmunoassay ◽  
Layer Chromatography

ABSTRACT The effect of Synacthen (β1–24-corticotrophin) on plasma testosterone and 4-androstene-3,17-dione concentrations in untreated patients with prostatic carcinoma, and in patients receiving endocrine therapy is described. An established specific radioimmunoassay was used for the measurement of testosterone, and a radioimmunoassay for 3-androstene-3,17-dione using thin layer chromatography has been developed. Administration of Synacthen resulted in a fall in testosterone in untreated patients, but a rise in 4-androstene-3,17-dione was observed. The plasma concentration of testosterone in all treated patients increased after administration of Synacthen. An increased concentration of plasma 4-androstene-3,17-dione was also observed in these treated patients after Synacthen, but the magnitude of the response was not significantly different from that of untreated patients. The work provides further evidence that in the patient being treated with oestrogen for carcinoma of the prostate a rise in plasma testosterone concentration will result from an increased secretion of ACTH.

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