PROGESTERONE, 20α-DIHYDROPROGRESTERONE AND 20β-DIHYDROPROGESTERONE LEVELS IN DIFFERENT COMPARTMENTS FROM THE HUMAN FOETO-PLACENTAL UNIT

1975 ◽  
Vol 80 (3) ◽  
pp. 558-568 ◽  
Author(s):  
B. Runnebaum ◽  
H. Runnebaum ◽  
I. Stöber ◽  
J. Zander
Keyword(s):  
Liquid Chromatography ◽  
Mass Spectrum ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Reductase Activity ◽  
Placental Tissue ◽  
Gas Liquid Chromatography ◽  
Wet Weight ◽  
Layer Chromatography ◽  
Foetal Blood

ABSTRACT The endogenous levels of progesterone, 20α-dihydroprogesterone and 20β-dihydroprogesterone in different foetal tissues, in placental tissue and in foetal blood during pregnancy (week 9–25) were determined by gas-liquid chromatography or by radioimmunoassay. The identification of these steroids in foetal tissues was based on the behaviour in paper and thin-layer chromatography, the formation of different derivatives, and the retention times in gas-liquid chromatography. For additional identification of progesterone and 20α-dihydroprogesterone a mass spectrum was obtained. The average concentrations of progesterone (P), 20α-dihydroprogesterone (20α-DHP) and 20β-dihydroprogesterone (20β-DHP) in foetal tissues were as follows (ng/g wet weight tissue): brain, P 119.0 ± 45.5, 20α-DHP 86.9 ± 44.7 and 20β-DHP 9.7; lungs, P 257.7 ± 195.7, 20α-DHP 60.3 ± 26.4 and 20β-DHP 7.8; liver, P 103.7 ± 84.6, 20α-DHP 95.2 ± 89.8 and 20β-DHP 17.8; adrenals, P 295.7 ± 90.0 and 20α-DHP 254.5 ± 194.6; kidneys, P 214.2 ± 155.3 and 20α-DHP 76.6 ± 43.0; intestine, P 273.2 ± 166.7, 20α-DHP 77.9 ± 51.2 and 20β-DHP 8.7; residual tissues, P 246.3 ± 178.3 and 20α-DHP 54.8 ± 44.5 The average concentrations in placental tissue and in foetal plasma (week 12–18) were as follows (ng/g wet weight tissue or ng/ml plasma): placenta, P 2619. ± 2153.6 and 20α-DHP 461.0 ± 155.9; foetal plasma, P 378.7 ± 231.8 and 20α-DHP 283.0 ± 108.9 These results indicate that some foetal organs contain a high 20α-reductase activity at least during the first half of pregnancy.

ANALYSIS OF CHLORORGANIC COMPOUNDS IN FODDER FOR BROILERCHICKENS BY THE METHOD OF GAS-LIQUID CHROMATOGRAPHY WITH MASSSPECTROMETER DETECTOR

2020 ◽  
Vol 1 (2) ◽  
pp. 205-208
Author(s):  
A.A. Parshutina ◽  
◽  
A.A. Solovyova ◽  
L.P. Satyukova ◽  
E.G. Shubina ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Organochlorine Pesticides ◽  
Mass Spectrometer ◽  
Broiler Chickens ◽  
Gas Liquid Chromatography ◽  
Layer Chromatography

The article shows the importance of the study of feed for broiler chickens on the content of organochlorine pesticides. These substances in significant concentrations can disrupt the development of birds and cause mass poisoning. The study of formula feed for broiler chickens «prestart» and «start» by two methodswas conducted: a certified method for the determination of organochlorine pesticides in feed and formula feed (thin layer chromatography) and a method for detecting pesticides not certified for feed (gas-liquid chromatography with mass spectrometer detector). During the experiment, the presence of organochlorine pesticides in several formulafeed samples was revealed.

Additional Cleanup of Samples on GLC Column for TLC Determination of Pesticide Residues

1967 ◽  
Vol 50 (3) ◽  
pp. 615-623
Author(s):  
Kenneth T Hartman
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Pesticide Residues ◽  
Gas Liquid Chromatography ◽  
Rf Values ◽  
Layer Chromatography

Abstract Gas-liquid chromatography (GLC) following conventional isolation procedures has been used to clean up pesticide residues for confirmation by thin layer chromatography (TLC). This procedure is more rapid and efficient than present cleanup procedures and permits the determination of pesticide residues that do not survive these rigorous acid or alkali treatments. The method also permits TLC confirmation of pesticide residues that have similar Rf values but different GLC retention times. Recoveries ranged from 85 to 105% for 25 of 28 pesticides tested

Thin Layer and Gas-Liquid Chromatography of Cholesterol in Fats and Oils. I. Development of Method

1969 ◽  
Vol 52 (4) ◽  
pp. 774-778
Author(s):  
C W Thorpe ◽  
Linda Pohland ◽  
D Firestone
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Unsaponifiable Matter ◽  
Fats And Oils ◽  
Cholesterol Content ◽  
Gas Liquid Chromatography ◽  
Animal Fat ◽  
Low Levels ◽  
Layer Chromatography

Abstract A inethod is described for analysis of sterols by thin layer chromatography-gas liquid chromatography (TLC-GLC). Sterols are isolated from other components of unsaponifiable matter by preparative TLC. The sterols are quantitatively removed from the TLC plate, extracted from the silica gel, and analyzed by GLC. This method has been used to detect low levels (2–3%) of animal fat in vegetable oil by measuring the cholesterol content of the animal fatvegetable oil admixtures.

Steroids in Aged Whisky

1973 ◽  
Vol 56 (6) ◽  
pp. 1357-1361
Author(s):  
Robert A Black ◽  
Arthur A Andreasen
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Gas Liquid Chromatography ◽  
Infrared Spectrophotometry ◽  
Layer Chromatography

Abstract Steroids were isolated from aged whisky and identified as β-sitosterol-D-glucoside, β-sitosterol, stigmasterol, and campesterol. Only β-sitosterol-D-glucoside has been reported in whisky. These steroids were characterized by gas-liquid chromatography, thin layer chromatography, and infrared spectrophotometry. They are extracted from the barrel during maturation and can contribute to clarity instability in finished whisky products.

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