The Rho GTPases RhoA and CDC42 mediate apoptosis by a combination of statins and zoledronic acid in human bone-seeking breast cancer cells

2016 ◽  
Author(s):  
Andy Gobel ◽  
Stefanie Thiele ◽  
Andrew J Browne ◽  
Martina Rauner ◽  
Lorenz C Hofbauer ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Zoledronic Acid ◽  
Cancer Cells ◽  
Rho Gtpases ◽  
Breast Cancer Cells ◽  
Human Bone

scholarly journals Constitutive p21-activated Kinase (PAK) Activation in Breast Cancer Cells as a Result of Mislocalization of PAK to Focal Adhesions

2004 ◽  
Vol 15 (6) ◽  
pp. 2965-2977 ◽  
Author(s):  
Mary R. Stofega ◽  
Luraynne C. Sanders ◽  
Elisabeth M. Gardiner ◽  
Gary M. Bokoch
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Rho Gtpases ◽  
Breast Cancer Cells ◽  
Kinase Activity ◽  
Focal Adhesions ◽  
Breast Cancer Cell Lines ◽  
Cytoskeletal Remodeling ◽  
P21 Activated Kinase ◽  
Pak Kinase

Cytoskeletal remodeling is critical for cell adhesion, spreading, and motility. p21-activated kinase (PAK), an effector molecule of the Rho GTPases Rac and Cdc42, has been implicated in cytoskeletal remodeling and cell motility. PAK kinase activity and subcellular distribution are tightly regulated by rapid and transient localized Rac and Cdc42 activation, and by interactions mediated by adapter proteins. Here, we show that endogenous PAK is constitutively activated in certain breast cancer cell lines and that this active PAK is mislocalized to atypical focal adhesions in the absence of high levels of activated Rho GTPases. PAK localization to focal adhesions in these cells is independent of PAK kinase activity, NCK binding, or GTPase binding, but requires the association of PAK with PIX. Disruption of the PAK–PIX interaction with competitive peptides displaces PAK from focal adhesions and results in a substantial reduction in PAK hyperactivity. Moreover, disruption of the PAK–PIX interaction is associated with a dramatic decrease of PIX and paxillin in focal adhesions, indicating that PAK localization to these structures via PIX is required for the maintenance of paxillin- and PIX-containing focal adhesions. Abnormal regulation of PAK localization and activity may contribute to the tumorigenic properties of certain breast cancer cells.

Abstract 4261: Anti-angiogenic effects of zoledronic acid on osteotropic breast cancer cells

2011 ◽  
Author(s):  
Cynthia Contreras ◽  
Walid Hamud ◽  
Bishal Dhakal ◽  
Bijay Khanal ◽  
Jason Bush
Keyword(s):  
Breast Cancer ◽  
Zoledronic Acid ◽  
Cancer Cells ◽  
Breast Cancer Cells

scholarly journals Antitumor activity of zoledronic acid in primary breast cancer cells determined by the ATP tumor chemosensitivity assay

BMC Cancer ◽  
2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Tanja Fehm ◽  
Manfred Zwirner ◽  
Diethelm Wallwiener ◽  
Harald Seeger ◽  
Hans Neubauer
Keyword(s):  
Breast Cancer ◽  
Zoledronic Acid ◽  
Antitumor Activity ◽  
Cancer Cells ◽  
Primary Breast Cancer ◽  
Breast Cancer Cells ◽  
Chemosensitivity Assay

85 Progenitor cells derived from human bone marrow contribute to the growth of breast cancer cells

2004 ◽  
Vol 2 (8) ◽  
pp. 29
Author(s):  
R. Bagley ◽  
A. Dash ◽  
X. Cao ◽  
T. Lodie ◽  
S. Madden ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Bone Marrow ◽  
Cancer Cells ◽  
Progenitor Cells ◽  
Breast Cancer Cells ◽  
Human Bone ◽  
Human Bone Marrow
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