Urinary excretion rate of ceruloplasmin in non-insulin-dependent diabetic patients with different stages of nephropathy

1995 ◽  
Vol 132 (6) ◽  
pp. 681-687 ◽  
Author(s):  
Masatoshi Yamazaki ◽  
Seiki Ito ◽  
Akio Usami ◽  
Nagayuki Tani ◽  
Osamu Hanyu ◽  
...  
Keyword(s):  
Urinary Excretion ◽  
Excretion Rate ◽  
University Hospital ◽  
Tubular Function ◽  
Dependent Diabetes Mellitus ◽  
Diabetic Patients ◽  
Urinary Excretion Rate ◽  
Serum Samples ◽  
Insulin Dependent ◽  
Insulin Dependent Diabetic

Yamazaki M, Ito S, Usami A, Tani N, Hanyu 0, Nakagawa O. Nakamura H, Shibata A. Urinary excretion rate of ceruloplasmin in non-insulin-dependent diabetic patients with different stages of nephropathy. Eur J Endocrinol 1995;132:681–7. ISSN 0804–4643 The level of ceruloplasmin, which is a more negatively charged protein than albumin, was measured by an immunoradiometric assay in timed overnight urine and serum samples from patients with non-insulin-dependent diabetes mellitus and healthy controls. None of the plasma proteins examined showed any cross-reactivity in this assay. A linear correlation was seen between the ceruloplasmin level and the serial dilution of the sample. Western blot analysis using concentrated urine samples showed that the molecular weight of ceruloplasmin in the urine sample was the same as that of ceruloplasmin in the serum and standard samples. These findings indicated that the substance detected by this assay was truly ceruloplasmin. The urinary ceruloplasmin excretion rate (CER) and clearance of ceruloplasmin increased in parallel with the progression of albuminuria. The highest CER was found in macroalbuminuric patients, followed by micro- and normoalbuminuric patients and the healthy control subjects, the differences between the groups being significant. In view of the fact that the isoelectric point of ceruloplasmin (4.4) is more acidic than that of albumin, the present findings suggested that an enhanced CER was due either to the alteration of charge selectivity in the glomerular basement membrane with unaltered tubular function or to a defect of the non-discriminatory pores (shunt pathway) with unaltered tubular function. Seiki Ito, Division of Gerontology, Akita University Hospital, 1-1-1 Hondou, Akita City, Japan 010

Urinary orosomucoid excretion rate in patients with non-insulin-dependent diabetes mellitus

1989 ◽  
Vol 120 (5) ◽  
pp. 584-590 ◽  
Author(s):  
Seiki Ito ◽  
Akiko Tsuda ◽  
Takeshi Momotsu ◽  
Kazumasa Igarashi ◽  
Shin Kasahara ◽  
...  
Keyword(s):  
Urinary Excretion ◽  
Excretion Rate ◽  
Albumin Excretion Rate ◽  
Insulin Dependent Diabetes Mellitus ◽  
Dependent Diabetes Mellitus ◽  
Diabetic Patients ◽  
Healthy Controls ◽  
Urinary Excretion Rate ◽  
Albumin Excretion ◽  
Insulin Dependent

Abstract. Urinary excretion rate and clearance of α1-acid glycoprotein (orosomucoid), a major serum glycoprotein which is more anionic (pI 2.7) than albumin (pI 4.7) were measured by RIA in timed overnight urine samples from non-insulin-dependent diabetic patients with different urinary albumin excretion rate and from healthy controls. The 50th percentiles of urinary orosomucoid excretion rate in patients with normo-, micro-, and macroalbuminuria were larger than those in healthy controls. Urinary excretion rate and clearance of orosomucoid increased in parallel with increase in albumin excretion rate in diabetic patients with an albumin excretion rate of more than 10 μg/min. On the basis of their levels of urinary orosomucoid excretion, patients with normoalbuminuria of less than 10 μg/min could be divided into two groups, one with a normal and the other with an elevated urinary orosomucoid excretion rate. The findings suggest that kidneys of diabetic patients with an albumin excretion rate of more than 10 μg/min are unable to distinguish the difference in pI between albumin and orosomucoid, and that a subgroup with an elevated orosomucoid excretion rate may be present among diabetics with normoalbuminuria.

Increased urinary excretion of digoxin-like immunoreactive substance by insulin-dependent diabetic patients: a linkage with hypertension?

1988 ◽  
Vol 34 (12) ◽  
pp. 2418-2422 ◽  
Author(s):  
O Giampietro ◽  
A Clerico ◽  
G Gregori ◽  
S Bertoli ◽  
M G Del Chicca ◽  
...  
Keyword(s):  
Body Weight ◽  
Urinary Excretion ◽  
Body Mass ◽  
Cardiac Glycoside ◽  
Excretion Rate ◽  
Diabetic Patients ◽  
Insulin Dependent ◽  
The Mean ◽  
Excretion Rates ◽  
Insulin Dependent Diabetic

Abstract Excretion of digoxin-like immunoreactivity (DLIS) was measured by RIA in timed overnight urine collections from 91 normotensive nondiabetic subjects and 104 normotensive insulin-dependent diabetic (IDDM) patients. The mean +/- SD DLIS excretion rate for the diabetic patients significantly exceeded that for the controls (73 +/- 41 vs 63 +/- 36 pg/min, P = 0.024). In both groups, the mean DLIS excretion rates for men were significantly higher (P = 0.0014, P = 0.006) than for women. In the controls, the DLIS excretion rate significantly correlated with the urinary excretion rate of creatinine (P less than 0.01), Na+ (P less than 0.05), and K+(P less than 0.05), and with the subjects' body weight (P less than 0.01), body mass index (P less than 0.05), and systolic blood pressure (P less than 0.05). In the diabetics, the DLIS excretion rate was significantly correlated with body weight (P less than 0.05) and with urinary excretion rates for albumin (P less than 0.01), creatinine (P less than 0.01), Na+ (P less than 0.05), and K+(P less than 0.05). Our data indicate that: (a) increased amounts of a cardiac glycoside-like substance (or a group of substances) are excreted in the urine of IDDM patients; (b) the urinary excretion of DLIS seems to depend on glomerular filtration rate and physiocochemical properties of glomerular membrane, as well as on subjects' body mass; and (c) because cardiac glycoside-like substances may increase peripheral vascular resistance, increased urinary excretion of DLIS by IDDM patients may indicate a tendency to develop hypertension.

Effect of Posture and Acute Glycaemic Control on the Excretion of Retinol-Binding Protein in Normoalbuminuric Insulin-Dependent Diabetic Patients

1993 ◽  
Vol 84 (4) ◽  
pp. 461-467 ◽  
Author(s):  
Carlo Catalano ◽  
Peter H. Winocour ◽  
Susan Gillespie ◽  
Ian Gibb ◽  
K. George M. M. Alberti
Keyword(s):  
Blood Glucose Concentration ◽  
Excretion Rate ◽  
Binding Protein ◽  
Insulin Dependent Diabetes ◽  
Retinol Binding Protein ◽  
Diabetic Patients ◽  
Control Subjects ◽  
Protein Excretion ◽  
Insulin Dependent ◽  
Insulin Dependent Diabetic

1. It has been suggested that tubular damage may precede gomerular damage at the onset of diabetic nephropathy. This may be reflected by increased urinary excretion of low-molecular-mass proteins, such as retinol-binding protein. 2. We have measured the urinary excretion rate of retinol-binding protein overnight, during orthostasis and during a hyperinsulinaemic euglycaemic clamp (blood glucose concentration 7.0 mmol/l) with stable diuresis in 34 normotensive, normoalbuminuric insulin-dependent diabetic patients and in 10 normal control subjects. Normal control subjects were not clamped. A further four normoalbuminuric insulin-dependent diabetic patients were rendered euglycaemic without a water load. 3. Overnight retinol-binding protein excretion rate was 58 (16-157) [median(range)] ng/min in patients with insulin-dependent diabetes and 32 (15-72) ng/min in control subjects (P < 0.01). The excretion rate did not change during orthostasis [patients with insulin-dependent diabetes, 67 (3-173) ng/min; control subjects, 23 (5-78) ng/min]. During the euglycaemic clamp retinol-binding protein excretion rate increased to 383 (78-4897) ng/min in patients with insulin-dependent diabetes (P < 0.01). An average increment in retinol-binding protein excretion rate of greater than 4000% was noted after acute euglycaemia in those patients with insulin-dependent diabetes who were not water-loaded. 4. In insulin-dependent diabetes, both overnight and orthostatic retinal-binding protein excretion was not correlated with fasting blood glucose concentration, HbA1, fructosamine or duration of diabetes. The absolute and incremental excretion rates of retinol-binding protein during the clamp were, however, correlated with both fasting blood glucose concentration and glucose excretion rate (rs = 0.41-0.48, P < 0.01). 5. The study demonstrates that retinol-binding protein excretion is increased in insulin-dependent diabetes in the absence of microalbuminuria and that this increase in retinol-binding protein excretion is particularly pronounced after acute euglycaemia. Acute tubular dysfunction related to acute changes in glucose control appears to be the most likely explanation, but established tubular damage could also be implicated. Postural variation in retinol-binding protein excretion was not detected.

Glomerular and Tubular Proteinuria in Type 1 (Insulin-Dependent) Diabetic Patients with and without Retinopathy

1992 ◽  
Vol 29 (3) ◽  
pp. 265-270 ◽  
Author(s):  
P Martin ◽  
H Tindall ◽  
J N Harvey ◽  
T M Handley ◽  
C Chapman ◽  
...  
Keyword(s):  
Urinary Excretion ◽  
Diabetic Patients ◽  
Tubular Proteinuria ◽  
Duration Of Diabetes ◽  
Albumin Excretion ◽  
Potential Index ◽  
Insulin Dependent ◽  
Insulin Dependent Diabetic ◽  
Prospective Longitudinal

We compared the urinary excretion of albumin, transferrin, N-acetyl-β-D-glucosaminidase and α-1-microglobulin in 78 Type 1 (insulin-dependent) diabetic patients: 39 with retinopathy and 39 without. The two groups were matched for age, sex and duration of diabetes. The patients with retinopathy had increased excretion (median and range) of albumin [1·7 (0·3–399·1) versus 1·0 (0·3–116·6) mg/mmol creatinine, P < 0·05], transferrin [114·2 (4·1–37126·2) versus 33·4 (1·0–4176·7) μg/mmol creatinine, P < 0·01] and N-acetyl-β-D-glucosaminidase [23·8 (1·1–119·1) versus 15·0 (0·1–65·1) μmol/h/mmol creatinine, P < 0·05] but not α-1-microglobulin. Transferrin excretion correlated with albumin excretion. The prevalence of increased transferrin excretion (transferrinuria) was greater than that of microalbuminuria in patients both with and without retinopathy ( P < 0·01 in both cases). Urinary transferrin seems likely to be predominantly of glomerular origin and merits prospective longitudinal evaluation as a potential index of the microangiopathic process.

Age Differences in Hopelessness and Toe Pain in Persons with Insulin-Dependent and Non-Insulin-Dependent Diabetes Mellitus

2010 ◽  
Vol 100 (6) ◽  
pp. 445-451 ◽  
Author(s):  
Stephen Morewitz ◽  
Najwa Javed ◽  
Sharada Tata ◽  
Joel Clark
Keyword(s):  
Depressive Symptoms ◽  
Peripheral Neuropathy ◽  
Age Differences ◽  
Null Hypothesis ◽  
Large Population ◽  
Dependent Diabetes Mellitus ◽  
Diabetic Patients ◽  
Insulin Dependent ◽  
Pain Symptoms ◽  
Insulin Dependent Diabetic

Background: Several studies have established an association between diabetic neuropathy and depressive symptoms. There is a link between depression and peripheral neuropathy in diabetic patients, suggesting an increased likelihood that diabetic patients will experience depressive symptoms related to lower-extremity peripheral neuropathy and arthritis during middle age and later life. The goal of this investigation was to determine whether there are age differences between insulin-dependent and non-insulin-dependent diabetic patients regarding their feelings of hopelessness and toe pain. Methods: A large population-based sample of 32,006 adults from the 1998 National Health Interview Survey was analyzed with multivariate statistical procedures. We performed χ2 and correlation procedures to test the null hypothesis that there are no age or sex differences between insulin-dependent and non-insulin-dependent diabetic patients in their reporting of feelings of hopelessness and toe pain symptoms in the previous 12 months. Results: There were significant differences between age and sex groups of insulin-dependent and non-insulin-dependent diabetic patients in reporting feelings of hopelessness and toe pain symptoms, rejecting the null hypothesis. Correlational analysis conducted between the variables of hopelessness and toe pain yielded significant correlations in insulin-dependent (r = .28; P = .0009; α = .05), and non-insulin-dependent (r = 0.19; P = .001; α = .05) women older than 61 years, concluding that diabetic women are more likely to experience hopelessness and toe pain in that age group regardless of insulin status. Conclusions: Clinicians should incorporate depression and toe pain symptoms into their assessment and treatment, especially in diabetic women older than 61 years. (J Am Podiatr Med Assoc 100(6): 445–451, 2010)

Urinary Excretion Rate and Clearance of IgG4and α2-Macroglobulin in Type 2 Diabetic Patients

1995 ◽  
Vol 27 (06) ◽  
pp. 303-304 ◽  
Author(s):  
S. Ito ◽  
M. Yamazaki ◽  
A. Usami ◽  
O. Hanyu ◽  
N. Tani ◽  
...  
Keyword(s):  
Urinary Excretion ◽  
Excretion Rate ◽  
Diabetic Patients ◽  
Urinary Excretion Rate ◽  
Type 2 Diabetic Patients ◽  
Α2 Macroglobulin ◽  
Type 2 Diabetic

Borderline maintenance of erythrocyte 2,3-diphosphoglycerate concentrations in normoxic type 1 (insulin dependent) diabetic subjects

1986 ◽  
Vol 70 (2) ◽  
pp. 127-129 ◽  
Author(s):  
C. J. Story ◽  
A. P. Roberts ◽  
R. G. Ryall
Keyword(s):  
Insulin Dependent Diabetes Mellitus ◽  
Dependent Diabetes Mellitus ◽  
Diabetic Patients ◽  
Haemoglobin A1c ◽  
Oxygen Dissociation Curve ◽  
Dissociation Curve ◽  
Oxygen Dissociation ◽  
Insulin Dependent ◽  
Insulin Dependent Diabetic

1. Erythrocyte 2,3-diphosphoglycerate and haemoglobin A1C concentrations were measured in 26 clinically normoxic patients with type 1 (insulin dependent) diabetes mellitus. The concentration of 2,3-diphosphoglycerate theoretically required to maintain normal erythrocyte oxygen delivery function in each subject was calculated and compared with the measured concentrations. 2. In the majority of diabetic patients 2,3-diphosphoglycerate concentrations were sufficient to keep the erythrocyte oxygen dissociation curve within the normal range under otherwise normal blood conditions. There was, however, a minority of patients in which this was not true. 3. It is concluded that the increased erythrocyte 2,3-diphosphoglycerate concentrations in clinically normoxic diabetic subjects are generally less than compensatory for the effect of haemoglobin A1C formation on the haemoglobin-oxygen dissociation curve.

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