Plasma levels of active form of ghrelin during oral glucose tolerance test in patients with anorexia nervosa

OBJECTIVE: Ghrelin is an acylated peptide, whose octanoyl modification is essential for its biological activities. Previous studies demonstrated that fasting plasma ghrelin levels were high in anorectic patients, suggesting ghrelin may play an important role in the pathophysiology of anorexia nervosa. However, antibodies used in previous work to measure ghrelin concentrations in human blood do not distinguish between the active form of ghrelin (active ghrelin) and desacyl ghrelin with no biological activities. Therefore, we studied plasma levels of active ghrelin during oral glucose tolerance test (OGTT) in anorectic patients, using a radioimmunoassay (RIA) specific for active ghrelin. METHODS: Active ghrelin response to OGTT was evaluated in five female anorectic patients and seven age-matched control females. All subjects were given a 75 g/225 ml glucose solution orally after overnight fasting. For RIA of active ghrelin, 1 N hydrogen chloride was added to the samples at final concentration of 0.1 N immediately after separation of plasma. RESULTS: Plasma basal levels of active ghrelin were significantly higher in anorectic patients than in controls (52.1+/-10.5 vs 21.2+/-3.1 fmol/ml, P<0.01). They were significantly decreased during OGTT in anorectic patients and in controls, reaching a nadir of 49.0+/-7.7% and 57.3+/-4.5% of the basal levels, respectively. CONCLUSION: These results suggest that hyperghrelinemia in anorectic patients is caused at least partly by increased secretion of active ghrelin and that glucose ingestion suppresses active ghrelin release in these patients.

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Short-term secretory regulation of the active form of ghrelin and total ghrelin during an oral glucose tolerance test in patients with anorexia nervosa

Acta Endocrinologica ◽

10.1530/eje.0.1500913 ◽

2004 ◽

pp. 913-914 ◽

Cited By ~ 13

Author(s):

Y Nakai ◽

H Hosoda ◽

K Nin ◽

C Ooya ◽

H Hayashi ◽

...

Keyword(s):

Anorexia Nervosa ◽

Glucose Tolerance ◽

Oral Glucose Tolerance Test ◽

Glucose Tolerance Test ◽

Active Form ◽

Tolerance Test ◽

Oral Glucose ◽

Oral Glucose Tolerance ◽

Short Term ◽

Total Ghrelin

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Plasma but not serum brain-derived neurotrophic factor concentration is decreased by oral glucose tolerance test-induced hyperglycemia in children

Journal of Pediatric Endocrinology and Metabolism ◽

10.1515/jpem-2016-0344 ◽

2017 ◽

Vol 30 (5) ◽

Cited By ~ 2

Author(s):

Shunsuke Araki ◽

Yukiyo Yamamoto ◽

Reiko Saito ◽

Aoi Kawakita ◽

Mami Eguchi ◽

...

Keyword(s):

Glucose Tolerance ◽

Oral Glucose Tolerance Test ◽

Neurotrophic Factor ◽

Plasma Levels ◽

Glucose Tolerance Test ◽

Serum Levels ◽

Brain Derived Neurotrophic Factor ◽

Tolerance Test ◽

Oral Glucose ◽

Oral Glucose Tolerance

AbstractBackground:Little is known regarding the relationships among circulating brain-derived neurotrophic factor (BDNF) levels and glucose or insulin in children and adolescents. The objective of this study was to investigate whether circulating BDNF levels would change during the oral glucose tolerance test (OGTT).Methods:We performed the OGTT and measured the serial changes in BDNF levels in both plasma and serum.Results:There were 22 subjects in the normal type (N) group and 20 in the borderline/diabetic type (B/D) group, defined by the results of the OGTT. Serum levels of BDNF were almost five times higher and plasma levels gradually decreased during the OGTT, whereas serum levels showed no significant change. The reduction of plasma BDNF level changes from baseline to 120 min were significantly different between the N and B/D groups (36.3% vs. 20.8%, p=0.023).Conclusions:Our results showed that plasma levels of BDNF are more sensitive to acute changes in glucose or insulin levels than serum.

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