scholarly journals A proton NMR study of the effect of a new intravasal injectable male contraceptive RISUG on seminal plasma metabolites

Reproduction ◽  
2001 ◽  
pp. 431-436 ◽  
Author(s):  
U Sharma ◽  
K Chaudhury ◽  
NR Jagannathan ◽  
SK Guha
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Seminal Plasma ◽  
Vas Deferens ◽  
Plasma Metabolites ◽  
Obstructive Azoospermia ◽  
Male Contraceptive ◽  
Nmr Study ◽  
Significant Difference ◽  
Styrene Maleic Anhydride ◽  
Prostatic Diseases

Nuclear magnetic resonance (NMR) spectroscopy was used to quantify citrate, glucose, lactate, glycerophosphorylcholine and choline in seminal plasma from subjects injected with a new male contraceptive RISUG, a copolymer of styrene maleic anhydride dissolved in dimethyl sulphoxide, and in seminal plasma from normal ejaculates. No significant difference in the concentration of citrate was observed between the groups, indicating that the prostate is not affected by the contraceptive. The concentrations of glucose, lactate, glycerophosphorylcholine and choline were significantly lower (P < 0.01) in subjects injected with RISUG compared with controls. In addition, metabolite ratios such as choline:citrate, citrate:lactate, choline:lactate and glycerophosphorylcholine:choline were calculated. Citrate:lactate and glycerophosphorylcholine:choline ratios were significantly lower in RISUG-injected subjects than in controls (P < 0.01), thereby indicating the occurrence of partial obstructive azoospermia. The most important finding of the present study was that the intervention of RISUG in the vas deferens even for a period as long as 8 years is absolutely safe and does not lead to prostatic diseases.

Use of epididymal sperm for assisted reproduction in men with acquired, irreparable obstructive azoospermia

1995 ◽  
Vol 7 (4) ◽  
pp. 841 ◽  
Author(s):  
P Patrizio ◽  
T Ord ◽  
JP Balmaceda ◽  
RH Asch
Keyword(s):  
Vas Deferens ◽  
Sperm Count ◽  
Fertilization Rate ◽  
Obstructive Azoospermia ◽  
Epididymal Sperm ◽  
Vitro Fertilization ◽  
Sperm Aspiration ◽  
Significant Difference ◽  
Vasectomy Reversal

Microsurgical epididymal sperm aspiration (MESA) and in vitro fertilization (IVF) is primarily offered to men with congenital absence of the vas deferens (CAVD). However, the IVF capacity of these epididymal sperm is low ( < 15%) and unpredictable. In this study, IVF and intracytoplasmic sperm injection (ICSI) results in patients with non-congenital, irreparable obstructive azoospermia were analysed. Thirty-three patients were evaluated for a total of 37 cycles of MESA and IVF. Most had obstruction secondary to failed vasectomy reversal and to epididymal blockage. The overall fertilization rate was 30% with regular IVF and 26% with ICSI, and six clinical pregnancies were obtained. Both rates are significantly higher than the IVF rate previously reported with sperm from men with CAVD (13%, P < 0.00001). In men with non-congenital obstructive azoospermia, a significant difference was found in the average sperm count (56.9 x 10(6) v. 12.3 x 10(6), P < 0.04) and total motile count (16.6 x 10(6) v. 1.6 x 10(6), P < 0.01) respectively for patients who achieved IVF and those who did not. It is concluded that (a) the real IVF capacity of human epididymal sperm is 30%, or 42%, if calculated only for patients who achieved fertilization, (b) this higher rate is an indirect support of the hypothesis that sperm from men with CAVD have intrinsic biochemical defects, related to cystic fibrosis mutations, responsible for their low and unpredictable IVF rate, and (c) MESA and IVF can be offered at the same time or as an alternative to patients requesting vasectomy reversal.

A proton NMR study of the effect of Mucuna pruriens on seminal plasma metabolites of infertile males

2011 ◽  
Vol 55 (5) ◽  
pp. 1060-1066 ◽  
Author(s):  
Ashish Gupta ◽  
Abbas Ali Mahdi ◽  
Mohammad Kaleem Ahmad ◽  
Kamla Kant Shukla ◽  
Navneeta Bansal ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Proton Nmr ◽  
Mucuna Pruriens ◽  
Plasma Metabolites ◽  
Nmr Study

Efficacy of Withania somnifera on seminal plasma metabolites of infertile males: A proton NMR study at 800MHz

2013 ◽  
Vol 149 (1) ◽  
pp. 208-214 ◽  
Author(s):  
Ashish Gupta ◽  
Abbas Ali Mahdi ◽  
Kamla Kant Shukla ◽  
Mohammad Kaleem Ahmad ◽  
Navneeta Bansal ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Withania Somnifera ◽  
Proton Nmr ◽  
Plasma Metabolites ◽  
Nmr Study

Nuclear magnetic resonance (NMR) study of 73Ge in Ge[Co2]O4

1973 ◽  
Vol 45 (6) ◽  
pp. 469-470 ◽  
Author(s):  
H. Saji
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Nmr Study ◽  
Nuclear Magnetic

scholarly journals 1H nuclear magnetic resonance of human seminal plasma in in vitro fertilization attempts: use of automatic spectrum analysis

Reproduction ◽  
1995 ◽  
Vol 103 (1) ◽  
pp. 181-187 ◽  
Author(s):  
J. Segalen ◽  
J. D. de Certaines ◽  
M. Le Calve ◽  
D. Colleu ◽  
J. Y. Bansard ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
In Vitro Fertilization ◽  
Seminal Plasma ◽  
Spectrum Analysis ◽  
Human Seminal Plasma ◽  
Vitro Fertilization ◽  
1H Nuclear Magnetic Resonance ◽  
Nuclear Magnetic

Nuclear magnetic resonance (NMR) study of HfV2

1972 ◽  
Vol 39 (5) ◽  
pp. 359-360 ◽  
Author(s):  
H. Saji ◽  
T. Yamadaya
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Nmr Study ◽  
Nuclear Magnetic

scholarly journals Sperm Metabolomics through Nuclear Magnetic Resonance Spectroscopy

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1669
Author(s):  
Marta Lombó ◽  
Sara Ruiz-Díaz ◽  
Alfonso Gutiérrez-Adán ◽  
María-Jesús Sánchez-Calabuig
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Nuclear Magnetic Resonance Spectroscopy ◽  
Current Knowledge ◽  
Proton Nuclear Magnetic Resonance ◽  
Farm Animals ◽  
Plasma Metabolites ◽  
Resonance Spectroscopy ◽  
Nuclear Magnetic

This report reviews current knowledge of sperm metabolomics analysis using proton nuclear magnetic resonance spectroscopy (1 H-NMR) with particular emphasis on human and farm animals. First, we present the benefits of NMR over other techniques to identify sperm metabolites and then describe the specific methodology required for NMR sperm analysis, stressing the importance of analyzing metabolites extracted from both the hydrophilic and lipophilic phases. This is followed by a description of advances produced to date in the use of NMR to diagnose infertility in humans and to identify metabolic differences among the sperm of mammalian herbivore, carnivore, and omnivore species. This last application of NMR mainly seeks to explore the possible use of lipids to fuel sperm physiology, contrary to previous theories that glycolysis and oxidative phosphorylation (OXPHOS) are the only sources of sperm energy. This review describes the use of NMR to identify sperm and seminal plasma metabolites as possible indicators of semen quality, and to examine the metabolites needed to maintain sperm motility, induce their capacitation, and consequently, to predict animal fertility.

Genetic mutation analysis of 22 patients with congenital absence of vas deferens: A single-center study

2021 ◽  
Author(s):  
Mao-Qing Tan ◽  
Wu-Jian Huang ◽  
Feng-Hua Lan ◽  
Yong-Jun Xu ◽  
Mei-Yu Zheng ◽  
...  
Keyword(s):  
Vas Deferens ◽  
Bioinformatic Analysis ◽  
Genetic Mutation ◽  
Congenital Absence ◽  
Chinese Patients ◽  
Obstructive Azoospermia ◽  
Missense Mutations ◽  
Transmembrane Conductance Regulator ◽  
Transmembrane Conductance ◽  
Single Center Study

Abstract Congenital absence of the vas deferens (CAVD), a congenital malformation of the male reproductive system, causes obstructive azoospermia and male infertility. Currently, the cystic fibrosis transmembrane conductance regulator (CFTR) has been recognized as the main pathogenic gene in CAVD, with some other genes, such as adhesion G-protein coupled receptor G2 (ADGRG2), solute carrier family 9 isoform 3 (SLC9A3), sodium channel epithelial 1 subunit beta (SCNN1B), and carbonic anhydrase 12 (CA12) being candidate genes in the pathogenesis of CAVD. However, the frequency and spectrum of these mutations, as well as the pathogenic mechanisms of CAVD, have not been fully investigated. Here, we sequenced all genes with potentially pathogenic mutations using next-generation sequencing and verified all identified variants by Sanger sequencing. Further bioinformatic analysis was performed to predict the pathogenicity of mutations. We described the distribution of the p.V470M, poly-T, and TG-repeat CFTR polymorphisms, and identified novel missense mutations in the CFTR and SLC9A3 genes, respectively. Taken together, we identified mutations in the CFTR, ADGRG2, SLC9A3, SCNN1B, and CA12 genes in 22 patients with CAVD, thus broadening the genetic spectrum of Chinese patients with CAVD.

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