The Influence of Halide and Nitrate Ions on Glucose Assay Using a Glucose Electrode

2005 ◽  
pp. 154-161
Author(s):  
M. Przybyt
Keyword(s):  
Glucose Assay ◽  
Nitrate Ions ◽  
Glucose Electrode

scholarly journals Interference in glucose and other clinical chemistry assays by thiocyanate and cyanide in a patient treated with nitroprusside

1996 ◽  
Vol 42 (3) ◽  
pp. 449-453 ◽  
Author(s):  
E W Randell ◽  
P St Louis
Keyword(s):  
Heart Failure ◽  
Congestive Heart Failure ◽  
Blood Glucose Concentration ◽  
Clinical Chemistry ◽  
Total Calcium ◽  
Aminotransferase Activity ◽  
Glucose Assay ◽  
Glucose Electrode ◽  
Toxic Concentrations ◽  
Alanine Aminotransferase Activity

Abstract A 3-year-old patient treated with nitroprusside for congestive heart failure had 6.5 mmol/L thiocyanate (toxic, >1.5 mmol/L) and 110 micromol/L cyanide (toxic, >5 micromol/L) present in her blood. At this time a whole-blood glucose concentration assayed on the Nova Stat Profile 5 Plus (Stat Profile) was 25.1 mmol/L. Plasma from that specimen analyzed on a Kodak Ektachem 700 analyzer (E700) indicated 5.2 mmol/L glucose. We investigated the potential interference of dissolved thiocyanate or cyanide on glucose and other routine assays. Toxic concentrations of thiocyanate increased Stat Profile glucose values and E700 total calcium, chloride, and creatinine values. Stat Profile ionized calcium values were decreased by toxic concentrations of thiocyanate. Cyanide (100 micromol/L) decreased alanine aminotransferase activity measured on the E700. Interference with the Stat Profile glucose assay may have been caused by thiocyanate oxidation at the glucose electrode.

scholarly journals Electrocatalytic Reduction of Hydrogen Peroxide on Palladium-Gold Codeposits on Glassy Carbon: Applications to the Design of Interference-Free Glucose Biosensor

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Elena Horozova ◽  
Totka Dodevska ◽  
Nina Dimcheva ◽  
Ruska Mussarlieva
Keyword(s):  
Hydrogen Peroxide ◽  
Glassy Carbon ◽  
Dynamic Range ◽  
Glucose Biosensor ◽  
Glassy Carbon Electrodes ◽  
Glucose Assay ◽  
Free Glucose ◽  
Pt Electrocatalysts ◽  
Glucose Electrode ◽  
Hydrogen Peroxide Electroreduction

Following our previous studies on the catalytic activity electrochemically codeposited on graphite Pd-Pt electrocatalysts for hydrogen peroxide electroreduction, a series of glassy carbon electrodes were modified with Pd or (Pd+Au) deposits aiming at the development of even more efficient electrocatalysts for the same process. The resulting electrodes were found to be very effective at low applied potentials (−100and−50 mV versus Ag/AgCl, 1 M KCl). The surface topography of the electrode modified with Pd+Au mixed in proportions 90% : 10%, exhibiting optimal combination of sensitivity and linear dynamic range towards hydrogen peroxide electrochemical reduction, was studied with SEM and AFM. The applicability of the same electrode as transducer in an amperometric biosensor for glucose assay was demonstrated. At an applied potential of−50 mV, the following were determined: detection limit (S/N=3) of6×10−6 M glucose, electrode sensitivity of 0.15 μAμM−1, and strict linearity up to concentration of3×10−4 M.

The influence of potassium dichromate on dissimilatory reduction of sulfate and nitrate ions by bacteria Desulfovibrio sp.

2017 ◽  
Vol 28 (1-2) ◽  
pp. 84-95
Author(s):  
O. M. Moroz ◽  
S. O. Hnatush ◽  
Ch. I. Bohoslavets ◽  
T. M. Hrytsun’ ◽  
B. M. Borsukevych
Keyword(s):  
Electron Acceptor ◽  
Potassium Dichromate ◽  
Anaerobic Respiration ◽  
Sulfate Reducing Bacteria ◽  
Negative Influence ◽  
Electron Acceptors ◽  
Metal Compounds ◽  
Nitrate Ions ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Sulfate reducing bacteria, capable to reductive transformation of different nature pollutants, used in biotechnologies of purification of sewage, contaminated by carbon, sulfur, nitrogen and metal compounds. H2S formed by them sediment metals to form of insoluble sulfides. Number of metals can be used by these microorganisms as electron acceptors during anaerobic respiration. Because under the influence of metal compounds observed slowing of bacteria metabolism, selection isolated from technologically modified ecotops resistant to pollutions strains is important task to create a new biotechnologies of purification. That’s why the purpose of this work was to study the influence of potassium dichromate, present in medium, on reduction of sulfate and nitrate ions by sulfate reducing bacteria Desulfovibrio desulfuricans IMV K-6, Desulfovibrio sp. Yav-6 and Desulfovibrio sp. Yav-8, isolated from Yavorivske Lake, to estimate the efficiency of possible usage of these bacteria in technologies of complex purification of environment from dangerous pollutants. Bacteria were cultivated in modified Kravtsov-Sorokin medium without SO42- and FeCl2×4H2O for 10 days. To study the influence of K2Cr2O7 on usage by bacteria SO42- or NO3- cells were seeded to media with Na2SO4×10H2O or NaNO3 and K2Cr2O7 at concentrations of 1.74 mM for total content of electron acceptors in medium 3.47 mM (concentration of SO42- in medium of standard composition). Cells were also seeded to media with 3.47 mM Na2SO4×10H2O, NaNO3 or K2Cr2O7 to investigate their growth in media with SO42-, NO3- or Cr2O72- as sole electron acceptor (control). Biomass was determined by turbidymetric method, content of sulfate, nitrate, dichromate, chromium (III) ions, hydrogen sulfide or ammonia ions in cultural liquid – by spectrophotometric method. It was found that K2Cr2O7 inhibits growth (2.2 and 1.3 times) and level of reduction by bacteria sulfate or nitrate ions (4.2 and 3.0 times, respectively) at simultaneous addition into cultivation medium of 1.74 mM SO42- or NO3- and 1.74 mM Cr2O72-, compared with growth and level of reduction of sulfate or nitrate ions in medium only with SO42- or NO3- as sole electron acceptor. Revealed that during cultivation of bacteria in presence of equimolar amount of SO42- or NO3- and Cr2O72-, last used by bacteria faster, content of Cr3+ during whole period of bacteria cultivation exceeded content H2S or NH4+. K2Cr2O7 in medium has most negative influence on dissimilatory reduction by bacteria SO42- than NO3-, since level of nitrate ions reduction by cells in medium with NO3- and Cr2O72- was a half times higher than level of sulfate ions reduction by it in medium with SO42- and Cr2O72-. The ability of bacteria Desulfovibrio sp. to priority reduction of Cr2O72- and after their exhaustion − NO3- and SO42- in the processes of anaerobic respiration can be used in technologies of complex purification of environment from toxic compounds.

Four methods for glucose assay compared for various glucose concentrations and under different clinical conditions.

1982 ◽  
Vol 28 (12) ◽  
pp. 2405-2407 ◽  
Author(s):  
O Giampietro ◽  
A Pilo ◽  
G Buzzigoli ◽  
C Boni ◽  
R Navalesi
Keyword(s):  
Glucose Oxidase ◽  
Glucose Concentration ◽  
Normal Subjects ◽  
Maintenance Hemodialysis ◽  
Oral Glucose ◽  
Glucose Assay ◽  
Glucose Tolerance Tests ◽  
Uremic Patients ◽  
Reducing Substances ◽  
Clinical Conditions

Abstract Glucose was measured by the ferricyanide, the Beckman glucose oxidase, and the hexokinase procedures in 228 plasma samples taken during standard oral glucose-tolerance tests in 17 normal subjects and in 21 chemical diabetics. The neocuproine method was also used to measure glucose concentration in 156 samples (78 before and 78 after dialysis) collected from six diabetic and uremic patients who were on maintenance hemodialysis. Ferricyanide in all conditions and neocuproine in uremic patients overestimated glucose concentrations over the entire experimental range as compared with either enzymic method. This bias or systematic error of the reducing vs the enzymic procedures, due to nonglucose reducing substances ("saccharoids"), becomes considerably greater when their concentration is increased as in chronic uremia. Also, the inverse relation between glucose concentration and overestimation of glucose by the reducing methods has been detected. With respect to the hexokinase method, a mild but significant underestimate of glucose oxidase readings has been observed for higher glucose concentrations. We find neocuproine to be the most imprecise of these procedures.

ChemInform Abstract: Reduction of Nitrate Ions into Nitrite and Ammonia Over Some Photocatalysts

ChemInform ◽  
2010 ◽  
Vol 23 (30) ◽  
pp. no-no
Author(s):  
A. KUDO ◽  
K. DOMEN ◽  
K. MARUYA ◽  
T. ONISHI
Keyword(s):  
Nitrate Ions
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