scholarly journals A Saturated Genetic Linkage Map of Autotetraploid Alfalfa (Medicago sativaL.) Developed Using Genotyping-by-Sequencing Is Highly Syntenous with theMedicago truncatulaGenome

2014 ◽  
Vol 4 (10) ◽  
pp. 1971-1979 ◽  
Author(s):  
Xuehui Li ◽  
Yanling Wei ◽  
Ananta Acharya ◽  
Qingzhen Jiang ◽  
Junmei Kang ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Genotyping By Sequencing

Construction of genetic linkage map using genotyping-by-sequencing and identification of QTLs associated with leaf color in spinach

Euphytica ◽  
2018 ◽  
Vol 214 (12) ◽  
Author(s):  
Xiaofeng Cai ◽  
Chenxi Xu ◽  
Xiaoli Wang ◽  
Shui Wang ◽  
Zhonghua Zhang ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Genotyping By Sequencing ◽  
Leaf Color

Genotyping-by-sequencing for construction of a new genetic linkage map and QTL analysis of growth-related traits in Pacific bluefin tuna

2017 ◽  
Vol 49 (3) ◽  
pp. 1293-1301 ◽  
Author(s):  
Tsubasa Uchino ◽  
Erina Hosoda ◽  
Yoji Nakamura ◽  
Motoshige Yasuike ◽  
Miyuki Mekuchi ◽  
...  
Keyword(s):  
Linkage Map ◽  
Qtl Analysis ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Genotyping By Sequencing ◽  
Bluefin Tuna ◽  
Pacific Bluefin Tuna

scholarly journals Construction of a high-density linkage map and detection of sex-specific markers in Penaeus japonicus

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12390
Author(s):  
Yaqun Zhang ◽  
Chuantao Zhang ◽  
Na Yao ◽  
Jingxian Huang ◽  
Xiangshan Sun ◽  
...  
Keyword(s):  
Sex Determination ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Molecular Mechanisms ◽  
Genetic Linkage Map ◽  
Genotyping By Sequencing ◽  
High Density ◽  
Nucleotide Polymorphisms ◽  
Penaeus Japonicus ◽  
Genetic Mechanisms

Penaeus japonicus is one of the most important farmed shrimp species in many countries. Sexual dimorphism is observed in P. japonicus, in which females grow faster and larger than males; therefore, a unisexual female culture of P. japonicus could improve the efficiency of productivity. However, the genetic mechanisms underlying sex determination in P. japonicus are unclear. In this study, we constructed a high-density genetic linkage map of P. japonicus using genotyping-by-sequencing (GBS) technology in a full-sib family. The final map was 3,481.98 cM in length and contained 29,757 single nucleotide polymorphisms (SNPs). These SNPs were distributed on 41 sex-averaged linkage groups, with an average inter-marker distance of 0.123 cM. One haplotype, harboring five sex-specific SNPs, was detected in linkage group 1 (LG1), and its corresponding confidence interval ranged from 211.840 to 212.592 cM. Therefore, this high-density genetic linkage map will be informative for genome assembly and marker-assisted breeding, and the sex-linked SNPs will be helpful for further studies on molecular mechanisms of sex determination and unisexual culture of P. japonicus in the future.

scholarly journals Construction of a high-density integrated genetic linkage map of rubber tree (Hevea brasiliensis) using genotyping-by-sequencing (GBS)

2015 ◽  
Vol 6 ◽  
Author(s):  
Wirulda Pootakham ◽  
Panthita Ruang-Areerate ◽  
Nukoon Jomchai ◽  
Chutima Sonthirod ◽  
Duangjai Sangsrakru ◽  
...  
Keyword(s):  
Linkage Map ◽  
Hevea Brasiliensis ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Rubber Tree ◽  
Genotyping By Sequencing ◽  
High Density

scholarly journals Construction of an Onion (Allium cepa L.) Genetic Linkage Map Using Genotyping-by-Sequencing Analysis with a Reference Gene Set and Identification of QTLs Controlling Anthocyanin Synthesis and Content

Plants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 616 ◽  
Author(s):  
Yousoo Choi ◽  
Sunggil Kim ◽  
Jundae Lee
Keyword(s):  
Linkage Map ◽  
Reference Gene ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Genotyping By Sequencing ◽  
Anthocyanin Synthesis ◽  
Anthocyanin Content ◽  
Marker Development ◽  
Linkage Groups ◽  
Gene Set

Anthocyanins, the pigmented flavonoids responsible for red and blue colors in horticultural products, promote human health by preventing cancers and lowering the risk of cardiovascular disease. Red onions contain several cyanidin- and peonidin-based anthocyanins. In this study, we constructed a single-nucleotide polymorphism (SNP)-based genetic linkage map in an F2 segregating population derived from a cross between the inbred line ‘SP3B’ (yellow bulb) and the doubled haploid line ‘H6′ (red bulb) to identify quantitative trait loci (QTLs) for total anthocyanin content of onion bulbs using a genotyping-by-sequencing (GBS) analysis based on a reference gene set. A total of 101.9 Gbp of raw sequences were generated using an Illumina HiSeq 2500 system and a total of 1625 SNP loci were identified with the criteria of three minimum depths, lower than 30% missing rate, and more than 5% minor allele frequency. As a result, an onion genetic linkage map consisting of 319 GBS-based SNP loci and 34 high-resolution melting (HRM) markers was constructed with eight linkage groups and a total genetic distance of 881.4 cM. In addition, the linkage groups were assigned to corresponding chromosomes by comparison with the reference genetic map OH1×5225 through marker development based on common transcripts. The analysis revealed one major QTL, qAS7.1, for anthocyanin synthesis and two significant QTLs, qAC4.1 and qAC4.2, for anthocyanin content. The QTL qAS7.1, located on chromosome 7 with a phenotypic variation of 87.61%, may be a dihydroflavonol 4-reductase (DFR) gene that determines whether the bulb color is red or yellow. The QTLs qAC4.1 and qAC4.2 are separately positioned on chromosome 4 with R2 values of 19.43% and 26.28%, respectively. This map and QTL information will contribute to marker development and breeding for high anthocyanin content in bulb onion.

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