scholarly journals Genomic Resources for Goniozus legneri,Aleochara bilineata and Paykullia maculata, Representing Three Independent Origins of the Parasitoid Lifestyle in Insects

2019 ◽  
pp. g3.300584.2019
Author(s):  
Ken Kraaijeveld ◽  
Peter Neleman ◽  
Janine Mariën ◽  
Emile de Meijer ◽  
Jacintha Ellers
2018 ◽  
Author(s):  
Ken Kraaijeveld ◽  
Peter Neleman ◽  
Janine Mariën ◽  
Emile de Meijer ◽  
Jacintha Ellers

AbstractParasitoid insects are important model systems for a multitude of biological research topics and widely used as biological control agents against insect pests. While the parasitoid lifestyle has evolved numerous times in different insect groups, research has focused almost exclusively on Hymenoptera from the parasitica clade. The genomes of several members of this group have been sequenced, but no genomic resources are available from any of the other, independent evolutionary origins of the parasitoid lifestyle. Our aim here was to develop genomic resources for three parasitoid insects outside the parasitica. We present draft genome assemblies forGoniozus legneri, a parasitoid Hymenopteran more closely related to the non-parasitoid wasps and bees than to the parasitica wasps, the Coleopteran parasitoidAleochara bilineataand the Dipteran parasitoidPaykullia maculata.The genome assemblies are fragmented, but complete in terms of gene content. We also provide preliminary structural annotations. We anticipate that these genomic resources will be valuable for testing the generality of findings obtained from parasitica wasps in future comparative studies.Data availabilityThe Whole Genome Shotgun projects have been deposited at DDBJ/ENA/GenBank under the accessions NCVS00000000 (G. legneri), NBZA00000000 (A. bilineata) and NDXZ00000000 (P. maculata).The versions described in this paper are versions NCVS01000000, NBZA01000000 and NDXZ01000000, respectively. Mapped reads and genome annotations are available throughhttp://parasitoids.labs.vu.nl/parasitoids/. This website also includes genome browsers and viroblast instances for each genome.


BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Kristen N. Finch ◽  
F. Andrew Jones ◽  
Richard C. Cronn

Plant Direct ◽  
2020 ◽  
Vol 4 (12) ◽  
Author(s):  
Ping Lou ◽  
Scott Woody ◽  
Kathleen Greenham ◽  
Robert VanBuren ◽  
Marivi Colle ◽  
...  

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1342
Author(s):  
Shaghayegh Mehravi ◽  
Gholam Ali Ranjbar ◽  
Ghader Mirzaghaderi ◽  
Anita Alice Severn-Ellis ◽  
Armin Scheben ◽  
...  

The species of Pimpinella, one of the largest genera of the family Apiaceae, are traditionally cultivated for medicinal purposes. In this study, high-throughput double digest restriction-site associated DNA sequencing technology (ddRAD-seq) was used to identify single nucleotide polymorphisms (SNPs) in eight Pimpinella species from Iran. After double-digestion with the enzymes HpyCH4IV and HinfI, a total of 334,702,966 paired-end reads were de novo assembled into 1,270,791 loci with an average of 28.8 reads per locus. After stringent filtering, 2440 high-quality SNPs were identified for downstream analysis. Analysis of genetic relationships and population structure, based on these retained SNPs, indicated the presence of three major groups. Gene ontology and pathway analysis were determined by using comparison SNP-associated flanking sequences with a public non-redundant database. Due to the lack of genomic resources in this genus, our present study is the first report to provide high-quality SNPs in Pimpinella based on a de novo analysis pipeline using ddRAD-seq. This data will enhance the molecular knowledge of the genus Pimpinella and will provide an important source of information for breeders and the research community to enhance breeding programs and support the management of Pimpinella genomic resources.


2021 ◽  
Author(s):  
Lars G. Kamphuis ◽  
Gagan Garg ◽  
Rhonda Foley ◽  
Karam B. Singh

Author(s):  
Venkateswara R. Sripathi ◽  
Ramesh Buyyarapu ◽  
Siva P. Kumpatla ◽  
Abreeotta J. Williams ◽  
Seloame T. Nyaku ◽  
...  

2021 ◽  
Author(s):  
Joel Berendzen ◽  
Anne V. Brown ◽  
Connor T. Cameron ◽  
Jacqueline D. Campbell ◽  
Alan M. Cleary ◽  
...  

Author(s):  
Romesh Kumar Salgotra ◽  
Rafiq Ahmad Bhat ◽  
Deyue Yu ◽  
Javaid Akhter Bhat

Abstract: Over the past two decades, the advances in the next generation sequencing (NGS) platforms have led to the identification of numerous genes/QTLs at high-resolution for their potential use in crop improvement. The genomic resources generated through these high-throughput sequencing techniques have been efficiently used in screening of particular gene of interest particularly for numerous types of plant stresses and quality traits. Subsequently, the identified-markers linked to a particular trait have been used in marker-assisted backcross breeding (MABB) activities. Besides, these markers are also being used to catalogue the food crops for detection of adulteration to improve the quality of food. With the advancement of technologies, the genomic resources are originating with new markers; however, to use these markers efficiently in crop breeding, high-throughput techniques (HTT) such as multiplex PCR and capillary electrophoresis (CE) can be exploited. Robustness, ease of operation, good reproducibility and low cost are the main advantages of multiplex PCR and CE. The CE is capable of separating and characterizing proteins with simplicity, speed and small sample requirements. Keeping in view the availability of vast data generated through NGS techniques and development of numerous markers, there is a need to use these resources efficiently in crop improvement programmes. In summary, this review describes the use of molecular markers in the screening of resistance genes in breeding programmes and detection of adulterations in food crops using high-throughput techniques.


2018 ◽  
pp. 13-55 ◽  
Author(s):  
Javaid Akhter Bhat ◽  
S. M. Shivaraj ◽  
Sajad Ali ◽  
Zahoor Ahmad Mir ◽  
Aminul Islam ◽  
...  

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