Tripsazea, a Novel Trihybrid of Zea mays, Tripsacum dactyloides, and Zeaperennis

A trispecific hybrid, MTP (hereafter called tripsazea), was developed from intergeneric crosses involving tetraploid Zea mays (2n = 4x = 40, genome: MMMM), tetraploid Tripsacum dactyloides (2n = 4x = 72, TTTT), and tetraploid Z. perennis (2n = 4x = 40, PPPP). On crossing maize-Tripsacum (2n = 4x = 56, MMTT) with Z. perennis, 37 progenies with varying chromosome numbers (36-74) were obtained, and a special one (i.e., tripsazea) possessing 2n = 74 chromosomes was generated. Tripsazea is perennial and expresses phenotypic characteristics affected by its progenitor parent. Flow cytometry analysis of tripsazea and its parents showed that tripsazea underwent DNA sequence elimination during allohexaploidization. Of all the chromosomes in diakinesis I, 18.42% participated in heterogenetic pairing, including 16.43% between the M- and P-genomes, 1.59% between the M- and T-genomes, and 0.39% in T- and P-genome pairing. Tripsazea is male sterile and partly female fertile. In comparison with previously synthesized trihybrids containing maize, Tripsacum and teosinte, tripsazea has a higher chromosome number, higher seed setting rate, and vegetative propagation ability of stand and stem. However, few trihybrids possess these valuable traits at the same time. The potential of tripsazea is discussed with respect to the deployment of the genetic bridge for maize improvement and forage breeding.

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A CYTOLOGICAL SURVEY OF ZEA-TRIPSACUM HYBRIDS

Canadian Journal of Genetics and Cytology ◽

10.1139/g73-090 ◽

1973 ◽

Vol 15 (4) ◽

pp. 763-778 ◽

Cited By ~ 9

Author(s):

C. A. Newell ◽

J. M. J. de Wet

Keyword(s):

Zea Mays ◽

Chromosome Number ◽

Zea Mays L ◽

Homologous Pairing ◽

Tripsacum Dactyloides ◽

Chromosome Behavior ◽

Chromosomal Homology ◽

Cytological Data ◽

Chromosomal Association ◽

Bivalent Formation

Reciprocal crosses involving various combinations of diploid (2n = 20) and tetraploid (2n = 40) Zea mays L. (maize), diploid (2n = 20) Zea mexicana (Schrad.) Kuntze (annual teosinte), and tetraploid (2n = 40) Zea perennis (Hitchc.) Reeves and Mangelsdorf (perennial teosinte) were studied cytologically. Observations of chromosome behavior during meiosis indicated that chromosomes of Z. perennis were more compatible with those of Zea mays than with those of Z. mexicana. Hybrids between Z. mays and diploid (2n = 36) or tetraploid (2n = 72) Tripsacum dactyloides (L.) L, incorporating different numbers of genomes from each parent and ranging in chromosome number from 2n = 28 28 to 2n = 82, were also examined at meiosis. Allodiploids (2n = 28) combining one Zea genome with one Tripsacum genome showed a low level of synaptic bivalent formation, indicating limited chromosomal homology between the two genera. Almost without exception, maize-Tripsacum hybrids exhibited more chromosomal association than expected on the basis of strictly homologous pairing; however, only a few plants possessed configurations stable enough to be taken as evidence of possible intergeneric segmental exchange. Triple hybrids involving maize, teosinte, and Tripsacum were produced in several combinations by pollinating maize-Tripsacum hybrids with annual or perennial teosinte. These showed chromosomal associations in accordance with the expected, on the basis of cytological data from maize-Tripsacum and maize-teosinte hybrids. These cytological observations suggest that teosinte and maize are conspecific, and that teosinte could not have originated as a by-product of Zea-Tripsacum introgression.

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Flow Cytometry Analysis of the Reactive Oxygen Species in Immature Granulocytes in Septic Patient

Case Medical Research ◽

10.31525/ct1-nct03846596 ◽

2000 ◽

Author(s):

Keyword(s):

Reactive Oxygen Species ◽

Flow Cytometry ◽

Septic Patient ◽

Reactive Oxygen ◽

Oxygen Species ◽

Flow Cytometry Analysis

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A Synergic Potential of Antimicrobial Peptides against Pseudomonas syringae pv. actinidiae

Molecules ◽

10.3390/molecules26051461 ◽

2021 ◽

Vol 26 (5) ◽

pp. 1461

Author(s):

Nuno Mariz-Ponte ◽

Laura Regalado ◽

Emil Gimranov ◽

Natália Tassi ◽

Luísa Moura ◽

...

Keyword(s):

Flow Cytometry ◽

Antimicrobial Peptides ◽

Pseudomonas Syringae ◽

Pathogenic Bacteria ◽

High Efficiency ◽

Bacterial Canker ◽

Membrane Permeation ◽

Flow Cytometry Analysis ◽

Pathogenic Agent ◽

Peptide Mixtures

Pseudomonas syringae pv. actinidiae (Psa) is the pathogenic agent responsible for the bacterial canker of kiwifruit (BCK) leading to major losses in kiwifruit productions. No effective treatments and measures have yet been found to control this disease. Despite antimicrobial peptides (AMPs) having been successfully used for the control of several pathogenic bacteria, few studies have focused on the use of AMPs against Psa. In this study, the potential of six AMPs (BP100, RW-BP100, CA-M, 3.1, D4E1, and Dhvar-5) to control Psa was investigated. The minimal inhibitory and bactericidal concentrations (MIC and MBC) were determined and membrane damaging capacity was evaluated by flow cytometry analysis. Among the tested AMPs, the higher inhibitory and bactericidal capacity was observed for BP100 and CA-M with MIC of 3.4 and 3.4–6.2 µM, respectively and MBC 3.4–10 µM for both. Flow cytometry assays suggested a faster membrane permeation for peptide 3.1, in comparison with the other AMPs studied. Peptide mixtures were also tested, disclosing the high efficiency of BP100:3.1 at low concentration to reduce Psa viability. These results highlight the potential interest of AMP mixtures against Psa, and 3.1 as an antimicrobial molecule that can improve other treatments in synergic action.

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